PMID- 24324399
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131210
LR  - 20211021
IS  - 1662-5099 (Print)
IS  - 1662-5099 (Electronic)
IS  - 1662-5099 (Linking)
VI  - 6
DP  - 2013
TI  - A comprehensive characterization of the nuclear microRNA repertoire of 
      post-mitotic neurons.
PG  - 43
LID - 10.3389/fnmol.2013.00043 [doi]
LID - 43
AB  - MicroRNAs (miRNAs) are small non-coding RNAs with important functions in the 
      development and plasticity of post-mitotic neurons. In addition to the 
      well-described cytoplasmic function of miRNAs in post-transcriptional gene 
      regulation, recent studies suggested that miRNAs could also be involved in 
      transcriptional and post-transcriptional regulatory processes in the nuclei of 
      proliferating cells. However, whether miRNAs localize to and function within the 
      nucleus of post-mitotic neurons is unknown. Using a combination of microarray 
      hybridization and small RNA deep sequencing, we identified a specific subset of 
      miRNAs which are enriched in the nuclei of neurons. Nuclear enrichment of 
      specific candidate miRNAs (miR-25 and miR-92a) could be independently validated 
      by Northern blot, quantitative real-time PCR (qRT-PCR) and fluorescence in situ 
      hybridization (FISH). By cross-comparison to published reports, we found that 
      nuclear accumulation of miRNAs might be linked to a down-regulation of miRNA 
      expression during in vitro development of cortical neurons. Importantly, by 
      generating a comprehensive isomiR profile of the nuclear and cytoplasmic 
      compartments, we found a significant overrepresentation of guanine nucleotides 
      (nt) at the 3'-terminus of nuclear-enriched isomiRs, suggesting the presence of 
      neuron-specific mechanisms involved in miRNA nuclear localization. In conclusion, 
      our results provide a starting point for future studies addressing the nuclear 
      function of specific miRNAs and the detailed mechanisms underlying subcellular 
      localization of miRNAs in neurons and possibly other polarized cell types.
FAU - Khudayberdiev, Sharof A
AU  - Khudayberdiev SA
AD  - Biochemisch-Pharmakologisches Centrum, Institut fur Physiologische Chemie, 
      Philipps-Universitat Marburg Marburg, Germany.
FAU - Zampa, Federico
AU  - Zampa F
FAU - Rajman, Marek
AU  - Rajman M
FAU - Schratt, Gerhard
AU  - Schratt G
LA  - eng
PT  - Journal Article
DEP - 20131126
PL  - Switzerland
TA  - Front Mol Neurosci
JT  - Frontiers in molecular neuroscience
JID - 101477914
PMC - PMC3840315
OTO - NOTNLM
OT  - deep sequencing
OT  - isomiR
OT  - miRNA
OT  - microarray
OT  - neuronal development
OT  - plasticity
EDAT- 2013/12/11 06:00
MHDA- 2013/12/11 06:01
PMCR- 2013/01/01
CRDT- 2013/12/11 06:00
PHST- 2013/09/16 00:00 [received]
PHST- 2013/11/07 00:00 [accepted]
PHST- 2013/12/11 06:00 [entrez]
PHST- 2013/12/11 06:00 [pubmed]
PHST- 2013/12/11 06:01 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - 10.3389/fnmol.2013.00043 [doi]
PST - epublish
SO  - Front Mol Neurosci. 2013 Nov 26;6:43. doi: 10.3389/fnmol.2013.00043. eCollection 
      2013.