PMID- 24325815
OWN - NLM
STAT- MEDLINE
DCOM- 20161114
LR  - 20211021
IS  - 1976-670X (Electronic)
IS  - 1976-6696 (Print)
IS  - 1976-6696 (Linking)
VI  - 49
IP  - 2
DP  - 2016 Feb
TI  - Non-histone protein HMGB1 inhibits the repair of damaged DNA by cisplatin in 
      NIH-3T3 murine fibroblasts.
PG  - 99-104
AB  - The nuclear non-histone protein high mobility group box (HMGB) 1 is known to 
      having an inhibitory effect on the repair of DNA damaged by the antitumor drug 
      cisplatin in vitro. To investigate the role of HMGB1 in living cells, we studied 
      the DNA repair of cisplatin damages in mouse fibroblast cell line, NIH-3T3. We 
      evaluated the effect of the post-synthetic acetylation and C-terminal domain of 
      the protein by overexpression of the parental and mutant GFP fused forms of 
      HMGB1. The results revealed that HMGB1 had also an inhibitory effect on the 
      repair of cisplatin damaged DNA in vivo. The silencing of HMGB1 in NIH-3T3 cells 
      increased the cellular DNA repair potential. The increased levels of repair 
      synthesis could be "rescued" and returned to less than normal levels if the 
      knockdown cells were transfected with plasmids encoding HMGB1 and HMGB1 K2A. In 
      this case, the truncated form of HMGB1 also exhibited a slight inhibitory effect. 
      [BMB Reports 2016; 49(2): 99-104].
FAU - Yusein-Myashkova, Shazie
AU  - Yusein-Myashkova S
AD  - Institute of Molecular Biology, Roumen Tsanev, Bulgarian Academy of Sciences, 
      Sofia 1113, Bulgaria.
FAU - Ugrinova, Iva
AU  - Ugrinova I
AD  - Institute of Molecular Biology, Roumen Tsanev, Bulgarian Academy of Sciences, 
      Sofia 1113, Bulgaria.
FAU - Pasheva, Evdokia
AU  - Pasheva E
AD  - Institute of Molecular Biology, Roumen Tsanev, Bulgarian Academy of Sciences, 
      Sofia 1113, Bulgaria.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - BMB Rep
JT  - BMB reports
JID - 101465334
RN  - 0 (Antineoplastic Agents)
RN  - 0 (HMGB1 Protein)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - Q20Q21Q62J (Cisplatin)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/pharmacology
MH  - Cisplatin/*pharmacology
MH  - *DNA Damage
MH  - DNA Repair/*drug effects
MH  - Fibroblasts/drug effects/*metabolism
MH  - Gene Knockdown Techniques
MH  - Green Fluorescent Proteins/metabolism
MH  - HMGB1 Protein/*metabolism
MH  - Mice
MH  - NIH 3T3 Cells
PMC - PMC4915123
EDAT- 2013/12/12 06:00
MHDA- 2016/11/15 06:00
PMCR- 2016/03/29
CRDT- 2013/12/12 06:00
PHST- 2013/10/30 00:00 [received]
PHST- 2013/12/12 06:00 [entrez]
PHST- 2013/12/12 06:00 [pubmed]
PHST- 2016/11/15 06:00 [medline]
PHST- 2016/03/29 00:00 [pmc-release]
AID - 2555 [pii]
AID - BMB-49-99 [pii]
AID - 10.5483/bmbrep.2016.49.2.238 [doi]
PST - ppublish
SO  - BMB Rep. 2016 Feb;49(2):99-104. doi: 10.5483/bmbrep.2016.49.2.238.