PMID- 24325829
OWN - NLM
STAT- MEDLINE
DCOM- 20140903
LR  - 20191210
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 945-946
DP  - 2014 Jan 15
TI  - Development of a method for the analysis of drugs of abuse in vitreous humor by 
      capillary electrophoresis with diode array detection (CE-DAD).
PG  - 84-91
LID - S1570-0232(13)00552-7 [pii]
LID - 10.1016/j.jchromb.2013.10.014 [doi]
AB  - This work presents the development of an analytical method based on capillary 
      electrophoresis with diode array detection for the analysis of drugs of abuse and 
      biotransformation products in vitreous humor. Composition of the background 
      electrolyte, implementation of an online pre-concentration strategy and sample 
      preparation procedures were objects of study. The complete electrophoretic 
      separation of 12 analytes (amphetamine, methamphetamine, 
      3,4-methylenedioxyamphetamine (MDA), 3,4-methylenedioxymethamphetamine (MDMA), 
      3,4-methylenedioxyethylamphetamine (MDEA), ketamine, cocaine, cocaethylene, 
      lidocaine, morphine, 6-monoacetylmorphine and heroin) and the internal standard 
      N-methyl-1-(3,4-methylenedioxyphenyl)-2-butamine (MBDB) was obtained within 13min 
      of run. The method was validated presenting good linearity (r(2)>0.99), recovery 
      >/=90%, precision better than 12% RSD and acceptable accuracy in the range of 
      86-118% at three concentration levels (50, 100 and 500ng/mL). LODs and LOQs in 
      the order of 1-5ng/mL and 5-10ng/mL, respectively, were obtained. After 
      validation, the method was applied to eighty-seven vitreous humor samples and the 
      results were compared to those obtained by a liquid chromatography tandem mass 
      spectrometry (LC-MS/MS) screening method, routinely used by the forensic 
      toxicology laboratory of the Sao Paulo State Police, Brazil. Cocaine was detected 
      in 7.1%, cocaethylene in 3.6%, lidocaine in 2.4% and ketamine in 1.2% of the 
      total number of analyzed samples.
CI  - Copyright (c) 2013. Published by Elsevier B.V.
FAU - Costa, Jose Luiz
AU  - Costa JL
AD  - Forensic Toxicology and Chemistry Laboratory, Criminalistics Institute of Sao 
      Paulo, Sao Paulo, SP, Brazil; Institute of Chemistry, University of Sao Paulo, 
      Sao Paulo, SP, Brazil.
FAU - Morrone, Andre Ribeiro
AU  - Morrone AR
AD  - Institute of Legal Medicine, Sao Paulo State Police, Sao Paulo, Brazil.
FAU - Resende, Rodrigo Ribeiro
AU  - Resende RR
AD  - Department of Biochemistry and Immunology, Institute of Biological Sciences, 
      Federal University of Minas Gerais, Belo Horizonte, Brazil.
FAU - Chasin, Alice Aparecida da Matta
AU  - Chasin AA
AD  - Faculty of Pharmaceutical Sciences, University of Sao Paulo, Sao Paulo, Brazil.
FAU - Tavares, Marina Franco Maggi
AU  - Tavares MF
AD  - Institute of Chemistry, University of Sao Paulo, Sao Paulo, SP, Brazil. 
      Electronic address: mfmtavar@iq.usp.br.
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20131021
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 0 (Illicit Drugs)
SB  - IM
MH  - Chromatography, Liquid
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Illicit Drugs/*analysis/isolation & purification
MH  - Limit of Detection
MH  - Substance Abuse Detection/*methods
MH  - Tandem Mass Spectrometry
MH  - Vitreous Body/*chemistry
OTO - NOTNLM
OT  - Capillary zone electrophoresis
OT  - Cocaine
OT  - Forensic toxicology
OT  - Liquid chromatography-mass spectrometry
OT  - Vitreous humor
EDAT- 2013/12/12 06:00
MHDA- 2014/09/04 06:00
CRDT- 2013/12/12 06:00
PHST- 2013/03/24 00:00 [received]
PHST- 2013/10/03 00:00 [revised]
PHST- 2013/10/12 00:00 [accepted]
PHST- 2013/12/12 06:00 [entrez]
PHST- 2013/12/12 06:00 [pubmed]
PHST- 2014/09/04 06:00 [medline]
AID - S1570-0232(13)00552-7 [pii]
AID - 10.1016/j.jchromb.2013.10.014 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 15;945-946:84-91. doi: 
      10.1016/j.jchromb.2013.10.014. Epub 2013 Oct 21.