PMID- 24326522 OWN - NLM STAT- MEDLINE DCOM- 20150212 LR - 20211021 IS - 1573-6830 (Electronic) IS - 0272-4340 (Linking) VI - 34 IP - 3 DP - 2014 Apr TI - Aminoguanidine changes hippocampal expression of apoptosis-related genes, improves passive avoidance learning and memory in streptozotocin-induced diabetic rats. PG - 343-50 LID - 10.1007/s10571-013-0018-5 [doi] AB - Cognitive dysfunction occurs in patients with diabetes mellitus. The objective of this study was to examine whether bilateral intrahippocampal CA1 (intra-CA1) injection of aminoguanidine (AG) can either affect the Bcl-2 family gene expression or reduce the diabetic imposing abnormalities of passive avoidance learning (PAL) and memory. Rats were divided into five groups: control (C), control treated with normal saline (CS), control treated with AG (S-AG), diabetics (D), and diabetics treated with AG (D-AG). Diabetes mellitus was induced by a single intraperitoneal injection of streptozotocin (STZ) (50 mg/kg). AG (30 mug/rat) or vehicle was administered intra-CA1 bilaterally at the onset of hyperglycemia. PAL was assessed 7 weeks later. Animals were killed, and hippocampus was dissected following the behavioral test. The expressions of Bax, Bcl-2, and Bcl-xl mRNAs were measured using semiquantitative RT-PCR technique. The result of passive avoidance task showed that AG significantly improved the cognitive performance in diabetic rats. Moreover, AG treatment decreased the levels of Bcl-2 and Bcl-xL expressions in diabetic group. The ratio of Bax/Bcl-2 and Bax/Bcl-xL decreased significantly in AG-treated diabetic animals. In conclusion, initial treatment with AG by intra-CA1 micro-injection improves the impaired passive avoidance task in STZ-induced diabetic rats which may be related to the decreased Bax/Bcl-2 and Bax/Bcl-xL ratios. FAU - Firouzjaei, Maryam Arab AU - Firouzjaei MA AD - Babol University of Medical Sciences, Babol, Iran. FAU - Jafari, Mohammad Reza AU - Jafari MR FAU - Eskandari, Mehdi AU - Eskandari M FAU - Anarkoli, Iraj Jafari AU - Anarkoli IJ FAU - Alipour, Mohsen AU - Alipour M LA - eng PT - Journal Article DEP - 20131211 PL - United States TA - Cell Mol Neurobiol JT - Cellular and molecular neurobiology JID - 8200709 RN - 0 (Apoptosis Regulatory Proteins) RN - 0 (Bax protein, mouse) RN - 0 (Bcl2l1 protein, mouse) RN - 0 (Guanidines) RN - 0 (Proto-Oncogene Proteins c-bcl-2) RN - 0 (bcl-2-Associated X Protein) RN - 0 (bcl-X Protein) RN - SCQ4EZQ113 (pimagedine) SB - IM MH - Animals MH - Apoptosis Regulatory Proteins/biosynthesis MH - Avoidance Learning/drug effects/*physiology MH - Diabetes Mellitus, Experimental/drug therapy/*metabolism MH - Gene Expression Regulation MH - Guanidines/pharmacology/*therapeutic use MH - Hippocampus/drug effects/metabolism MH - Male MH - Memory/drug effects/*physiology MH - Proto-Oncogene Proteins c-bcl-2/biosynthesis MH - Random Allocation MH - Rats MH - Rats, Wistar MH - bcl-2-Associated X Protein/*biosynthesis MH - bcl-X Protein/*biosynthesis EDAT- 2013/12/12 06:00 MHDA- 2015/02/13 06:00 CRDT- 2013/12/12 06:00 PHST- 2013/10/10 00:00 [received] PHST- 2013/12/04 00:00 [accepted] PHST- 2013/12/12 06:00 [entrez] PHST- 2013/12/12 06:00 [pubmed] PHST- 2015/02/13 06:00 [medline] AID - 10.1007/s10571-013-0018-5 [doi] PST - ppublish SO - Cell Mol Neurobiol. 2014 Apr;34(3):343-50. doi: 10.1007/s10571-013-0018-5. Epub 2013 Dec 11.