PMID- 24326625
OWN - NLM
STAT- MEDLINE
DCOM- 20150127
LR  - 20220311
IS  - 1590-3478 (Electronic)
IS  - 1590-1874 (Linking)
VI  - 35
IP  - 5
DP  - 2014 May
TI  - Effect of treadmill exercise on 5-HT, 5-HT1A receptor and brain derived neurophic 
      factor in rats after permanent middle cerebral artery occlusion.
PG  - 761-6
LID - 10.1007/s10072-013-1599-y [doi]
AB  - It has been well documented that exercise promotes neurological rehabilitation in 
      patients with cerebral ischemia. However, the exact mechanisms have not been 
      fully elucidated. This study aimed to discuss the effect of treadmill exercise on 
      expression levels of 5-HT, 5-HT1A receptor (5-HT1AR) and brain derived neurophic 
      factor (BDNF) in rat brains after permanent middle cerebral artery occlusion 
      (pMCAO). A total of 55 rats were randomly divided into 3 groups: pMCAO group, 
      pMCAO and treadmill exercise (pMCAO + Ex) group, and sham-operated group. Rats in 
      pMCAO + Ex group underwent treadmill exercise for 16 days. Neurological function 
      was evaluated by modified Neurological Severity Scores (mNSS). High-performance 
      liquid chromatography-electrochemical detection system was used to determine the 
      content of 5-HT in cortex tissues. The protein levels of 5-HT1AR, BDNF and 
      synaptophysin were measured by Western blot. The mNSS in pMCAO + Ex group was 
      lower than that in pMCAO group on day 19 post-MCAO (p < 0.001). The content of 
      5-HT dropped to 3.81 +/- 1.86 ng/ml in pMCAO group (43.84 +/- 2.05 ng/ml in 
      sham-operated group), but increased in pMCAO + Ex group (10.06 +/- 1.80 ng/ml). The 
      protein expressions levels of synaptophysin, 5-HT1AR and BDNF were downregulated 
      after cerebral ischemia (p < 0.05), and upregulated after treadmill exercise (p < 
      0.05). These results indicate that treadmill exercise improves neurologic 
      function, enhances neuronal plasticity and upregulates the levels of 5-HT, 
      5-HT1AR and BDNF in rats with pMCAO.
FAU - Lan, Xiaofang
AU  - Lan X
AD  - Department of Neurology, The First Affiliated Hospital, Chongqing Medical 
      University, 1st Youyi Road, Yuzhong District, Chongqing, 400016, People's 
      Republic of China.
FAU - Zhang, Meng
AU  - Zhang M
FAU - Yang, Wan
AU  - Yang W
FAU - Zheng, Zongju
AU  - Zheng Z
FAU - Wu, Yuan
AU  - Wu Y
FAU - Zeng, Qian
AU  - Zeng Q
FAU - Liu, Shudong
AU  - Liu S
FAU - Liu, Ke
AU  - Liu K
FAU - Li, Guangqin
AU  - Li G
LA  - eng
PT  - Journal Article
DEP - 20131211
PL  - Italy
TA  - Neurol Sci
JT  - Neurological sciences : official journal of the Italian Neurological Society and 
      of the Italian Society of Clinical Neurophysiology
JID - 100959175
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Synaptophysin)
RN  - 0 (Syp protein, rat)
RN  - 112692-38-3 (Receptor, Serotonin, 5-HT1A)
RN  - 333DO1RDJY (Serotonin)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Brain/pathology/*physiopathology
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Disease Models, Animal
MH  - *Exercise Therapy
MH  - Infarction, Middle Cerebral Artery/pathology/physiopathology/*therapy
MH  - Male
MH  - Motor Activity/physiology
MH  - Random Allocation
MH  - Rats, Sprague-Dawley
MH  - Receptor, Serotonin, 5-HT1A/*metabolism
MH  - Serotonin/*metabolism
MH  - Severity of Illness Index
MH  - Synaptophysin/metabolism
EDAT- 2013/12/12 06:00
MHDA- 2015/01/28 06:00
CRDT- 2013/12/12 06:00
PHST- 2013/07/13 00:00 [received]
PHST- 2013/11/28 00:00 [accepted]
PHST- 2013/12/12 06:00 [entrez]
PHST- 2013/12/12 06:00 [pubmed]
PHST- 2015/01/28 06:00 [medline]
AID - 10.1007/s10072-013-1599-y [doi]
PST - ppublish
SO  - Neurol Sci. 2014 May;35(5):761-6. doi: 10.1007/s10072-013-1599-y. Epub 2013 Dec 
      11.