PMID- 24328676
OWN - NLM
STAT- MEDLINE
DCOM- 20140919
LR  - 20140127
IS  - 1532-2513 (Electronic)
IS  - 0892-3973 (Linking)
VI  - 36
IP  - 1
DP  - 2014 Feb
TI  - beta-sitosteryl-3-O-beta-glucopyranoside isolated from the bark of Sorbus commixta 
      ameliorates pro-inflammatory mediators in RAW 264.7 macrophages.
PG  - 70-7
LID - 10.3109/08923973.2013.866956 [doi]
AB  - The bark of Sorbus commixta has been used in Asian traditional medicine for 
      treatment of cough, asthma, bronchial disorders, gastritis and dropsy. However, 
      the anti-inflammatory effect of beta-sitosteryl-3-O-beta-glucopyranoside, a major 
      compound of the bark of S. commixta, is poorly understood. In this study, we 
      investigated the anti-inflammatory effect and the underlying molecular mechanisms 
      of beta-sitosteryl-3-O-beta-glucopyranoside in lipopolysaccharide (LPS)-induced RAW 
      264.7 cells. Prostaglandin E(2) (PGE(2)) and cytokines released from cells were 
      measured using EIA assay kit. The expression of inducible NO synthase (iNOS) and 
      cyclooxygenase (COX)-2, Tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta 
      (IL-1beta), and interleukin-6 (IL-6) was measured by real-time polymerase chain 
      reaction (RT-PCR) and/or Western blot analysis. 
      beta-sitosteryl-3-O-beta-glucopyranoside inhibited the production of nitric oxide (NO) 
      and PGE(2) along with the expression of iNOS and COX-2 in LPS-stimulated RAW264.7 
      cells. In addition, beta-sitosteryl-3-O-beta-glucopyranoside reduced the release of 
      pro-inflammatory cytokines, such as TNF-alpha, IL-1beta and IL-6. Moreover, 
      beta-sitosteryl-3-O-beta-glucopyranoside inhibited the NF-kappaB activation induced by LPS, 
      which was associated with the abrogation of IkappaBalpha degradation and subsequent 
      decreases in nuclear p65 levels. The result suggested that the 
      beta-sitosteryl-3-O-beta-glucopyranoside inhibited NO and pro-inflammatory productions 
      by down-regulating the gene expression of pro-inflammatory mediators via the 
      negative regulation of the NF-small ka, CyrillicB pathway in LPS-stimulated RAW 264.7 cells.
FAU - Yang, Gabsik
AU  - Yang G
AD  - Department of Pharmacology, College of Oriental Medicine, Sangji University , 
      Gangwon-do , Republic of Korea.
FAU - An, Hyo-Jin
AU  - An HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131216
PL  - England
TA  - Immunopharmacol Immunotoxicol
JT  - Immunopharmacology and immunotoxicology
JID - 8800150
RN  - 0 (Cytokines)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Sitosterols)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 5LI01C78DD (gamma-sitosterol)
RN  - EC 1.14.99.- (Ptgs2 protein, mouse)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Cyclooxygenase 2/immunology
MH  - Cytokines/immunology
MH  - Inflammation Mediators/*immunology
MH  - Lipopolysaccharides/toxicity
MH  - Mice
MH  - Nitric Oxide/immunology
MH  - Plant Bark/*chemistry
MH  - Sitosterols/chemistry/*pharmacology
MH  - Sorbus/*chemistry
EDAT- 2013/12/18 06:00
MHDA- 2014/09/23 06:00
CRDT- 2013/12/17 06:00
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
AID - 10.3109/08923973.2013.866956 [doi]
PST - ppublish
SO  - Immunopharmacol Immunotoxicol. 2014 Feb;36(1):70-7. doi: 
      10.3109/08923973.2013.866956. Epub 2013 Dec 16.