PMID- 24329959
OWN - NLM
STAT- MEDLINE
DCOM- 20141020
LR  - 20140304
IS  - 1600-0560 (Electronic)
IS  - 0303-6987 (Linking)
VI  - 41
IP  - 3
DP  - 2014 Mar
TI  - Fully automated dual-color dual-hapten silver in situ hybridization staining for 
      MYC amplification: a diagnostic tool for discriminating secondary angiosarcoma.
PG  - 286-92
LID - 10.1111/cup.12278 [doi]
AB  - BACKGROUND: MYC amplification occurs in post-radiation and chronic 
      lymphedema-associated secondary angiosarcoma and some primary angiosarcomas. In 
      this study, we tested the ability of automated dual-color dual-hapten in situ 
      hybridization (DISH) staining to discriminate secondary angiosarcoma from 
      radiation-associated atypical vascular lesions (AVL), and to correlate with 
      fluorescence in situ hybridization (FISH) for MYC amplification. METHODS: Cases 
      of secondary angiosarcoma, including 11 biopsies and 3 excisions from 11 
      patients, and 5 AVL biopsies from 5 patients, were examined by FISH and DISH. 
      DISH staining was performed using the Dual Color Open Probe software on a Ventana 
      Benchmark XT automated slide stainer. Metallic black silver (MYC) and reference 
      CHR8 red signals were qualitatively and semi-quantitatively enumerated for tumor 
      nuclei. Small and large clusters of silver signals were recorded as 6 or 12 
      signals, respectively. MYC amplification was defined as MYC/CHR8 ratio >2.0. 
      RESULTS: Where tissue was available for both DISH and FISH, all secondary 
      angiosarcoma cases showed MYC amplification (11/11 = 100%) by both DISH and FISH. 
      All AVL were negative for MYC amplification by both techniques (0/5 = 0%). 
      CONCLUSION: In the current cohort, use of DISH identified all MYC amplified 
      cases, and distinguished secondary angiosarcoma from AVL. DISH staining may be 
      useful in distinguishing secondary angiosarcoma from AVL in challenging cases.
CI  - (c) 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Ko, Jennifer S
AU  - Ko JS
AD  - Pathology and Laboratory Medicine Institute, Cleveland Clinic, Cleveland, OH, 
      USA.
FAU - Billings, Steven D
AU  - Billings SD
FAU - Lanigan, Christopher P
AU  - Lanigan CP
FAU - Buehler, Darya
AU  - Buehler D
FAU - Fernandez, Anthony P
AU  - Fernandez AP
FAU - Tubbs, Raymond R
AU  - Tubbs RR
LA  - eng
PT  - Journal Article
DEP - 20140122
PL  - United States
TA  - J Cutan Pathol
JT  - Journal of cutaneous pathology
JID - 0425124
RN  - 0 (MYC protein, human)
RN  - 0 (Proto-Oncogene Proteins c-myc)
RN  - 3M4G523W1G (Silver)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - *Gene Amplification
MH  - *Hemangiosarcoma/genetics/metabolism/pathology
MH  - Humans
MH  - In Situ Hybridization/*methods
MH  - Male
MH  - Middle Aged
MH  - *Neoplasms, Radiation-Induced/genetics/metabolism/pathology
MH  - *Proto-Oncogene Proteins c-myc/genetics/metabolism
MH  - Retrospective Studies
MH  - Silver/*chemistry
MH  - *Skin Neoplasms/genetics/metabolism/pathology
OTO - NOTNLM
OT  - MYC
OT  - atypical vascular lesions
OT  - in situ hybridization
OT  - secondary angiosarcoma
OT  - soft tissue tumors
EDAT- 2013/12/18 06:00
MHDA- 2014/10/21 06:00
CRDT- 2013/12/17 06:00
PHST- 2013/08/19 00:00 [received]
PHST- 2013/11/14 00:00 [revised]
PHST- 2013/11/17 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/10/21 06:00 [medline]
AID - 10.1111/cup.12278 [doi]
PST - ppublish
SO  - J Cutan Pathol. 2014 Mar;41(3):286-92. doi: 10.1111/cup.12278. Epub 2014 Jan 22.