PMID- 24330147
OWN - NLM
STAT- MEDLINE
DCOM- 20161107
LR  - 20161230
IS  - 1601-0825 (Electronic)
IS  - 1354-523X (Linking)
VI  - 21
IP  - 1
DP  - 2015 Jan
TI  - IL-1beta-induced matrix metalloproteinase-3 regulates cell proliferation in rat 
      dental pulp cells.
PG  - 97-105
LID - 10.1111/odi.12219 [doi]
AB  - OBJECTIVE: We previously reported that matrix metalloproteinase-3(MMP-3) 
      accelerates wound healing following dental pulp injury. In this study, we tested 
      the hypothesis that induction of MMP-3 activity by interleukin-1beta would promote 
      proliferation and apoptosis of dental pulp cells. MATERIALS AND METHODS: Dental 
      pulp cells were isolated from rat incisors and subjected to interleukin-1beta. 
      Matrix metalloproteinase-3 mRNA and protein expression were assessed using 
      reverse transcription-polymerase chain reaction and Western blotting, 
      respectively. Matrix metalloproteinase-3 activity was measured using 
      fluorescence. Dental pulp cell proliferation and apoptosis were determined using 
      enzyme-linked immunosorbent assays (ELISA) for BrdU and DNA fragmentation, 
      respectively. siRNA was used to reduce MMP-3 transcripts in these cells. RESULTS: 
      Treatment with interleukin-1beta increased MMP-3 mRNA and protein levels as well as 
      its activity in dental pulp cells. Cell proliferation was also markedly 
      increased, with no changes in apoptosis observed. Treatment with siRNA against 
      MMP-3 potently suppressed this interleukin-1beta-induced increase in MMP-3 
      expression and activity, and also suppressed cell proliferation but unexpectedly 
      increased apoptosis in these cells (P < 0.05). This siRNA-mediated increase in 
      apoptosis could be reversed with exogenous MMP-3 stimulation (P < 0.05). 
      CONCLUSIONS: Interleukin-1beta induces MMP-3-regulated cell proliferation and 
      suppresses apoptosis in dental pulp cells.
CI  - (c) 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Ozeki, N
AU  - Ozeki N
AD  - Department of Endodontics, School of Dentistry, Aichi Gakuin University, Nagoya, 
      Japan.
FAU - Yamaguchi, H
AU  - Yamaguchi H
FAU - Hiyama, T
AU  - Hiyama T
FAU - Kawai, R
AU  - Kawai R
FAU - Nakata, K
AU  - Nakata K
FAU - Mogi, M
AU  - Mogi M
FAU - Nakamura, H
AU  - Nakamura H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131230
PL  - Denmark
TA  - Oral Dis
JT  - Oral diseases
JID - 9508565
RN  - 0 (Interleukin-1beta)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
MH  - Animals
MH  - Apoptosis/drug effects/physiology
MH  - Blotting, Western
MH  - Cell Proliferation/drug effects/*physiology
MH  - Dental Pulp/cytology/drug effects/*physiology
MH  - Interleukin-1beta/*pharmacology
MH  - Matrix Metalloproteinase 3/*physiology
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
OTO - NOTNLM
OT  - IL-1beta
OT  - cytokines
OT  - dental pulp cells
OT  - inflammation
OT  - matrix metalloproteinases
EDAT- 2013/12/18 06:00
MHDA- 2016/11/08 06:00
CRDT- 2013/12/17 06:00
PHST- 2013/04/12 00:00 [received]
PHST- 2013/11/12 00:00 [revised]
PHST- 2013/12/08 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2016/11/08 06:00 [medline]
AID - 10.1111/odi.12219 [doi]
PST - ppublish
SO  - Oral Dis. 2015 Jan;21(1):97-105. doi: 10.1111/odi.12219. Epub 2013 Dec 30.