PMID- 24330281
OWN - NLM
STAT- MEDLINE
DCOM- 20140404
LR  - 20211021
IS  - 1471-2377 (Electronic)
IS  - 1471-2377 (Linking)
VI  - 13
DP  - 2013 Dec 13
TI  - Detection of human herpesviruses in the cerebrospinal fluid from patients 
      diagnosed with or suspected of having progressive multifocal leukoencephalopathy.
PG  - 200
LID - 10.1186/1471-2377-13-200 [doi]
AB  - BACKGROUND: Progressive multifocal leukoencephalopathy (PML), a fatal 
      demyelinating disease caused by JC virus (JCV), occurs mainly in 
      immunocompromised patients. While JCV DNA is detected in the cerebrospinal fluid 
      (CSF) from a certain proportion of patients suspected of having PML, JCV-negative 
      patients may also develop brain lesions due to other infectious agents. This 
      study assessed the prevalence of six herpesviruses in the CSF from patients 
      diagnosed with or suspected of PML. METHODS: Two hundred and ninety-nine CSF 
      specimens and clinical data were collected from 255 patients, including 31 
      confirmed PML cases. Quantitative PCR assays were carried out to detect the 
      genomic DNA of JCV, herpes simplex virus (HSV), varicella-zoster virus (VZV), 
      cytomegalovirus (CMV), Epstein-Barr virus (EBV), and human herpesvirus 6 (HHV-6). 
      RESULTS: Herpesvirus DNAs were detected in the CSF specimens from 29 of 255 
      patients (11.4%). HSV-1 and CMV were detected in JCV-negative patients, whereas 
      VZV and EBV were detected in both CSF JCV-positive and -negative individuals. The 
      herpesvirus-positive patients had underlying disorders that caused 
      immunosuppression, such as HIV infection, congenital immunodeficiencies, and 
      hematologic malignancies, and presented with neurologic symptoms and MRI lesions, 
      mainly in the cerebral white matter. The median values of CSF cell counts and 
      protein levels in the herpesvirus-positive patients were slightly higher than 
      those in the PML patients. CONCLUSIONS: The results demonstrate that 
      herpesviruses are occasionally detected in the CSF from PML patients and 
      immunocompromised individuals suspected of having PML. Thus, this study provides 
      a significant basis for the diagnosis and treatment of neurological disorders in 
      immunocompromised patients.
FAU - Nakamichi, Kazuo
AU  - Nakamichi K
AD  - Department of Virology 1, National Institute of Infectious Diseases, Toyama 
      1-23-1, Shinjuku-ku, Tokyo 162-8640, Japan. nakamich@nih.go.jp.
FAU - Inoue, Naoki
AU  - Inoue N
FAU - Shimokawa, Toshio
AU  - Shimokawa T
FAU - Kurane, Ichiro
AU  - Kurane I
FAU - Lim, Chang-Kweng
AU  - Lim CK
FAU - Saijo, Masayuki
AU  - Saijo M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131213
PL  - England
TA  - BMC Neurol
JT  - BMC neurology
JID - 100968555
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/*cerebrospinal fluid
MH  - Female
MH  - Humans
MH  - JC Virus/*genetics
MH  - Leukoencephalopathy, Progressive Multifocal/*cerebrospinal fluid/*virology
MH  - Male
MH  - Middle Aged
MH  - Young Adult
PMC - PMC3878787
EDAT- 2013/12/18 06:00
MHDA- 2014/04/05 06:00
PMCR- 2013/12/13
CRDT- 2013/12/17 06:00
PHST- 2013/09/26 00:00 [received]
PHST- 2013/12/05 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/04/05 06:00 [medline]
PHST- 2013/12/13 00:00 [pmc-release]
AID - 1471-2377-13-200 [pii]
AID - 10.1186/1471-2377-13-200 [doi]
PST - epublish
SO  - BMC Neurol. 2013 Dec 13;13:200. doi: 10.1186/1471-2377-13-200.