PMID- 24330640
OWN - NLM
STAT- MEDLINE
DCOM- 20141104
LR  - 20220310
IS  - 1478-6362 (Electronic)
IS  - 1478-6354 (Print)
IS  - 1478-6354 (Linking)
VI  - 15
IP  - 6
DP  - 2013
TI  - Inducing articular cartilage phenotype in costochondral cells.
PG  - R214
AB  - INTRODUCTION: Costochondral cells may be isolated with minimal donor site 
      morbidity and are unaffected by pathologies of the diarthrodial joints. 
      Identification of optimal exogenous stimuli will allow abundant and robust 
      hyaline articular cartilage to be formed from this cell source. METHODS: In a 
      three factor, two level full factorial design, the effects of hydrostatic 
      pressure (HP), transforming growth factor beta1 (TGF-beta1), and chondroitinase ABC 
      (C-ABC), and all resulting combinations, were assessed in third passage expanded, 
      redifferentiated costochondral cells. After 4 wks, the new cartilage was assessed 
      for matrix content, superficial zone protein (SZP), and mechanical properties. 
      RESULTS: Hyaline articular cartilage was generated, demonstrating the presence of 
      type II collagen and SZP, and the absence of type I collagen. TGF-beta1 upregulated 
      collagen synthesis by 175% and glycosaminoglycan synthesis by 75%, resulting in a 
      nearly 200% increase in tensile and compressive moduli. C-ABC significantly 
      increased collagen content, and fibril density and diameter, leading to a 125% 
      increase in tensile modulus. Hydrostatic pressure increased fibril diameter by 
      30% and tensile modulus by 45%. Combining TGF-beta1 with C-ABC synergistically 
      increased collagen content by 300% and tensile strength by 320%, over control. No 
      significant differences were observed between C-ABC/TGF-beta1 dual treatment and 
      HP/C-ABC/TGF-beta1. CONCLUSIONS: Employing biochemical, biophysical, and mechanical 
      stimuli generated robust hyaline articular cartilage with a tensile modulus of 2 
      MPa and a compressive instantaneous modulus of 650 kPa. Using expanded, 
      redifferentiated costochondral cells in the self-assembling process allows for 
      recapitulation of robust mechanical properties, and induced SZP expression, key 
      characteristics of functional articular cartilage.
FAU - Murphy, Meghan K
AU  - Murphy MK
FAU - DuRaine, Grayson D
AU  - DuRaine GD
FAU - Reddi, A
AU  - Reddi A
FAU - Hu, Jerry C
AU  - Hu JC
FAU - Athanasiou, Kyriacos A
AU  - Athanasiou KA
LA  - eng
GR  - R01 AR061496/AR/NIAMS NIH HHS/United States
GR  - R01 DE015038/DE/NIDCR NIH HHS/United States
GR  - R01AR061496/AR/NIAMS NIH HHS/United States
GR  - R01DE019666/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - England
TA  - Arthritis Res Ther
JT  - Arthritis research & therapy
JID - 101154438
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cartilage, Articular/*cytology/physiology
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation
MH  - Chondrocytes/*cytology/physiology
MH  - Collagen/metabolism
MH  - Compressive Strength
MH  - Elastic Modulus
MH  - Microscopy, Electron, Scanning
MH  - Phenotype
MH  - Ribs/cytology
MH  - Sus scrofa
MH  - Tensile Strength
MH  - Tissue Engineering/*methods
PMC - PMC3979093
EDAT- 2013/12/18 06:00
MHDA- 2014/11/05 06:00
PMCR- 2013/12/12
CRDT- 2013/12/17 06:00
PHST- 2013/07/04 00:00 [received]
PHST- 2013/11/26 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/11/05 06:00 [medline]
PHST- 2013/12/12 00:00 [pmc-release]
AID - ar4409 [pii]
AID - 10.1186/ar4409 [doi]
PST - ppublish
SO  - Arthritis Res Ther. 2013;15(6):R214. doi: 10.1186/ar4409.