PMID- 24330824
OWN - NLM
STAT- MEDLINE
DCOM- 20140625
LR  - 20211021
IS  - 1755-8794 (Electronic)
IS  - 1755-8794 (Linking)
VI  - 6
DP  - 2013 Dec 15
TI  - Correlation of WWOX, RUNX2 and VEGFA protein expression in human osteosarcoma.
PG  - 56
LID - 10.1186/1755-8794-6-56 [doi]
AB  - BACKGROUND: To investigate associations between WW domain-containing 
      oxidoreductase (WWOX), runt-related transcription factor 2 (RUNX2) and vascular 
      endothelial growth factor alpha (VEGFA) in human osteosarcoma (OS). METHODS: Copy 
      number aberrations of WWOX, RUNX2and VEGFA genes were detected by microarray 
      comparative genomic hybridization (aCGH) in 10 fresh OS tissue samples. VEGFA 
      gene alterations were also investigated and validated by fluorescence in situ 
      hybridization (FISH) in 54 formalin-fixed and paraffin-embedded (FFPE) OS 
      samples. Protein expression of WWOX, RUNX2 and VEGFA were examined in 54 FFPE OS 
      samples by immunohistochemistry (IHC). RESULTS: Analysis of previously published 
      OS aCGH data (GSE9654) and aCGH data from this study (GSE19180) identified 
      significant deletion of WWOX in 30% (6/20) of OS samples, whilst significant 
      increase in both RUNX2 and VEGFA gene copy numbers were detected in 55% (11/20) 
      and 60% (12/20) of OS samples, respectively. FISH demonstrated increased VEGFA 
      gene copy number in 65.9% (31/47) of evaluable samples, in either focal or large 
      fragment forms. Compared with positive expression of WWOX in 38.9% of the OS 
      samples, positive expression of RUNX2 and VEGFA protein was found in 48.1 and 
      75.9% of samples. Although there was no significant association between gene copy 
      number aberration and protein expression for WWOX and RUNX2, significant positive 
      correlation between increased VEGFA gene copy number and VEGFA protein expression 
      was observed. Although there was no significant reverse association between WWOX 
      and RUNX2 expression, a significantly positive relationship was observed between 
      RUNX2 and VEGFA protein expression. CONCLUSIONS: Our data show increased RUNX2 
      and VEGFA gene copy numbers and elevation of their respective proteins in human 
      OS. Positive correlation of RUNX2 and VEGFA suggests that both increased VEGFA 
      gene copy number and RUNX2 overexpression facilitate increased expression of 
      VEGFA.
FAU - Yang, Jilong
AU  - Yang J
AD  - Department of Bone and Soft Tissue Tumor, Tianjin Medical University Cancer 
      Hospital & Institute, Tianjin 300060, China. yangjilong@tjmuch.com.
FAU - Zhao, Linru
AU  - Zhao L
FAU - Tian, Wei
AU  - Tian W
FAU - Liao, Zhichao
AU  - Liao Z
FAU - Zheng, Hong
AU  - Zheng H
FAU - Wang, Guowen
AU  - Wang G
FAU - Chen, Kexin
AU  - Chen K
LA  - eng
GR  - CA016672/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20131215
PL  - England
TA  - BMC Med Genomics
JT  - BMC medical genomics
JID - 101319628
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 1.- (Oxidoreductases)
RN  - EC 1.1.1.- (WW Domain-Containing Oxidoreductase)
RN  - EC 1.1.1.- (WWOX protein, human)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Bone Neoplasms/*genetics/metabolism/pathology
MH  - Core Binding Factor Alpha 1 Subunit/genetics/*metabolism
MH  - Female
MH  - Gene Dosage
MH  - *Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Osteosarcoma/*genetics/metabolism/pathology
MH  - Oxidoreductases/genetics/*metabolism
MH  - Tumor Suppressor Proteins/genetics/*metabolism
MH  - Vascular Endothelial Growth Factor A/genetics/*metabolism
MH  - WW Domain-Containing Oxidoreductase
MH  - Young Adult
PMC - PMC3878685
EDAT- 2013/12/18 06:00
MHDA- 2014/06/26 06:00
PMCR- 2013/12/15
CRDT- 2013/12/17 06:00
PHST- 2013/01/05 00:00 [received]
PHST- 2013/12/10 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/06/26 06:00 [medline]
PHST- 2013/12/15 00:00 [pmc-release]
AID - 1755-8794-6-56 [pii]
AID - 10.1186/1755-8794-6-56 [doi]
PST - epublish
SO  - BMC Med Genomics. 2013 Dec 15;6:56. doi: 10.1186/1755-8794-6-56.