PMID- 24333125
OWN - NLM
STAT- MEDLINE
DCOM- 20140908
LR  - 20211021
IS  - 1873-4863 (Electronic)
IS  - 0168-1656 (Print)
IS  - 0168-1656 (Linking)
VI  - 171
DP  - 2014 Feb 10
TI  - RNAi-based inhibition of porcine reproductive and respiratory syndrome virus 
      replication in transgenic pigs.
PG  - 17-24
LID - S0168-1656(13)00527-0 [pii]
LID - 10.1016/j.jbiotec.2013.11.022 [doi]
AB  - Porcine reproductive and respiratory syndrome (PRRS) is an economically 
      devastating viral disease causing heavy losses to the swine industry worldwide. 
      Many studies have shown that transient delivery of small interfering RNA (siRNA) 
      or adenovirus-mediated RNA interfere (RNAi) could potentially inhibit porcine 
      reproductive and respiratory syndrome virus (PRRSV) replication in vivo and in 
      vitro. Here, we applied RNAi to produce transgenic (TG) pigs that constitutively 
      expressed PRRSV-specific siRNA derived from small hairpin RNA (shRNA). First, we 
      evaluated siRNA expression in the founding and F1 generation pigs and confirmed 
      stable transmission. Then, we detected the expression of IFN-beta and protein kinase 
      R (PKR) and found no difference among TG, non-transgenic (NTG), and wild-type 
      pigs. Lastly, the F1 generation pigs, including TG and NTG piglets, were 
      challenged with 3x10(4).(5) TCID(5)(0) of JXA1, a highly pathogenic porcine reproductive 
      and respiratory syndrome virus (HP-PRRSV). Our results showed that the in vivo 
      siRNA expression substantially reduced the serum HP-PRRSV titers and increased 
      survival time by 3 days when TG pigs were compared with the NTG controls. These 
      data suggested that RNAi-based genetic modification might be used to breed 
      viral-resistant livestock with stable siRNA expression with no complications of 
      siRNA toxicity.
CI  - Copyright (c) 2013. Published by Elsevier B.V.
FAU - Li, Li
AU  - Li L
AD  - State Key Laboratories of Agrobiotechnology, College of Biological Science, China 
      Agricultural University, Beijing 100193, China; Department of Molecular Biology, 
      College of Biological Science, China Agricultural University, Beijing 100193, 
      China.
FAU - Li, Qiuyan
AU  - Li Q
AD  - State Key Laboratories of Agrobiotechnology, College of Biological Science, China 
      Agricultural University, Beijing 100193, China; Department of Molecular Biology, 
      College of Biological Science, China Agricultural University, Beijing 100193, 
      China.
FAU - Bao, Yonghua
AU  - Bao Y
AD  - State Key Laboratories of Agrobiotechnology, College of Biological Science, China 
      Agricultural University, Beijing 100193, China; Department of Molecular Biology, 
      College of Biological Science, China Agricultural University, Beijing 100193, 
      China.
FAU - Li, Jinxiu
AU  - Li J
AD  - State Key Laboratories of Agrobiotechnology, College of Biological Science, China 
      Agricultural University, Beijing 100193, China; Department of Molecular Biology, 
      College of Biological Science, China Agricultural University, Beijing 100193, 
      China.
FAU - Chen, Zhisheng
AU  - Chen Z
AD  - State Key Laboratories of Agrobiotechnology, College of Biological Science, China 
      Agricultural University, Beijing 100193, China; Department of Molecular Biology, 
      College of Biological Science, China Agricultural University, Beijing 100193, 
      China.
FAU - Yu, Xiuling
AU  - Yu X
AD  - OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China 
      Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing 
      100125, China.
FAU - Zhao, Yaofeng
AU  - Zhao Y
AD  - State Key Laboratories of Agrobiotechnology, College of Biological Science, China 
      Agricultural University, Beijing 100193, China; Department of Molecular Biology, 
      College of Biological Science, China Agricultural University, Beijing 100193, 
      China.
FAU - Tian, Kegong
AU  - Tian K
AD  - OIE Porcine Reproductive and Respiratory Syndrome Reference Laboratory, China 
      Animal Disease Control Center, No. 20 Maizidian Road, Chaoyang District, Beijing 
      100125, China; National Research Center for Veterinary Medicine, Cuiwei Road, 
      High-Tech. District, Luoyang, Henan Provice, China.
FAU - Li, Ning
AU  - Li N
AD  - State Key Laboratories of Agrobiotechnology, College of Biological Science, China 
      Agricultural University, Beijing 100193, China; Department of Molecular Biology, 
      College of Biological Science, China Agricultural University, Beijing 100193, 
      China. Electronic address: ninglcau@cau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131211
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (RNA, Small Interfering)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified/*genetics
MH  - Cells, Cultured
MH  - Female
MH  - Macrophages, Alveolar
MH  - Male
MH  - Nuclear Transfer Techniques
MH  - Porcine Reproductive and Respiratory Syndrome/genetics/prevention & control
MH  - Porcine respiratory and reproductive syndrome virus/*genetics
MH  - *RNA Interference
MH  - RNA, Small Interfering/*genetics
MH  - Survival Analysis
MH  - Swine/genetics
PMC - PMC7127135
OTO - NOTNLM
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - RNA interference
OT  - Somatic cell nuclear transplantation (SCNT)
OT  - Transgenic pig
EDAT- 2013/12/18 06:00
MHDA- 2014/09/10 06:00
PMCR- 2013/12/11
CRDT- 2013/12/17 06:00
PHST- 2013/07/28 00:00 [received]
PHST- 2013/11/22 00:00 [revised]
PHST- 2013/11/26 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/09/10 06:00 [medline]
PHST- 2013/12/11 00:00 [pmc-release]
AID - S0168-1656(13)00527-0 [pii]
AID - 10.1016/j.jbiotec.2013.11.022 [doi]
PST - ppublish
SO  - J Biotechnol. 2014 Feb 10;171:17-24. doi: 10.1016/j.jbiotec.2013.11.022. Epub 
      2013 Dec 11.