PMID- 2433337
OWN - NLM
STAT- MEDLINE
DCOM- 19870302
LR  - 20061115
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 138
IP  - 3
DP  - 1987 Feb 1
TI  - Tumor necrosis factor enhances HLA-A,B,C and HLA-DR gene expression in human 
      tumor cells.
PG  - 975-80
AB  - Tumor necrosis factor-alpha (TNF-alpha) exerts multiple biologic activities, 
      which include such diverse effects as direct tumoricidal activity and stimulation 
      of metabolic activities as well as expression of cell surface antigens on 
      nonmalignant cells. We report here that in various human tumor cells TNF-alpha 
      upregulates constitutively expressed HLA genes. Further, in a priori HLA-negative 
      tumor cells, TNF-alpha enhances IFN-gamma-induced class I and class II HLA gene 
      expression. In vitro transcription, Northern blot, and immunofluorescence 
      analysis performed with the leukemic cell line K 562 reveal that TNF-alpha 
      reversibly enhances IFN-gamma-induced HLA gene expression at the level of mRNA 
      transcription. In addition, a posttranscriptional regulation of HLA gene 
      expression by TNF-alpha is suggested from enhancement of HLA class I membrane 
      expression in Colo 205 cells without measurable changes in steady state mRNA 
      levels. Therefore, it is conceivable that TNF-alpha, similar to IFN-gamma, might 
      provoke tumor rejection not only via direct tumoricidal action but also via 
      enhancement of HLA antigens and induction of tumor-specific immune responses.
FAU - Pfizenmaier, K
AU  - Pfizenmaier K
FAU - Scheurich, P
AU  - Scheurich P
FAU - Schluter, C
AU  - Schluter C
FAU - Kronke, M
AU  - Kronke M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Glycoproteins)
RN  - 0 (HLA Antigens)
RN  - 0 (HLA-A Antigens)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-C Antigens)
RN  - 0 (HLA-D Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9008-11-1 (Interferons)
SB  - IM
MH  - Cell Line
MH  - Glycoproteins/*pharmacology
MH  - HLA Antigens/*genetics
MH  - HLA-A Antigens
MH  - HLA-B Antigens
MH  - HLA-C Antigens
MH  - HLA-D Antigens/*genetics
MH  - HLA-DR Antigens/*genetics
MH  - Humans
MH  - Interferons/pharmacology
MH  - Neoplasms/*immunology/metabolism
MH  - RNA, Messenger/analysis
MH  - Transcription, Genetic/*drug effects
MH  - Tumor Necrosis Factor-alpha
EDAT- 1987/02/01 00:00
MHDA- 1987/02/01 00:01
CRDT- 1987/02/01 00:00
PHST- 1987/02/01 00:00 [pubmed]
PHST- 1987/02/01 00:01 [medline]
PHST- 1987/02/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1987 Feb 1;138(3):975-80.