PMID- 24333760
OWN - NLM
STAT- MEDLINE
DCOM- 20140502
LR  - 20211021
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Print)
IS  - 0014-4835 (Linking)
VI  - 120
DP  - 2014 Mar
TI  - Acridine orange leukocyte fluorography in mice.
PG  - 15-9
LID - S0014-4835(13)00346-1 [pii]
LID - 10.1016/j.exer.2013.12.002 [doi]
AB  - Simultaneous non-invasive visualization of blood vessels and nerves in patients 
      can be obtained in the eye. The retinal vasculature is a target of many 
      retinopathies. Inflammation, readily manifest by leukocyte adhesion to the 
      endothelial lining, is a key pathophysiological mechanism of many retinopathies, 
      making it a valuable and ubiquitous target for disease research. Leukocyte 
      fluorography has been extensively used in the past twenty years; however, 
      fluorescent markers, visualization techniques, and recording methods have 
      differed between studies. The lack of detailed protocol papers regarding 
      leukocyte fluorography, coupled with lack of uniformity between studies, has led 
      to a paucity of standards for leukocyte transit (velocity, adherence, 
      extravasation) in the retina. Here, we give a detailed description of a 
      convenient method using acridine orange (AO) and a commercially available 
      scanning laser ophthalmoscope (SLO, HRA-OCT Spectralis) to view leukocyte 
      behavior in the mouse retina. Normal mice are compared to mice with acute and 
      chronic inflammation. This method can be readily adopted in many research labs.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Cahoon, Judd M
AU  - Cahoon JM
AD  - Department of Ophthalmology and Visual Science, John A Moran Eye Center, 
      University of Utah, Salt Lake City, UT 84109, United States. Electronic address: 
      judd.cahoon@hsc.utah.edu.
FAU - Olson, Paul R
AU  - Olson PR
AD  - Department of Ophthalmology and Visual Science, John A Moran Eye Center, 
      University of Utah, Salt Lake City, UT 84109, United States.
FAU - Nielson, Spencer
AU  - Nielson S
AD  - Department of Ophthalmology and Visual Science, John A Moran Eye Center, 
      University of Utah, Salt Lake City, UT 84109, United States.
FAU - Miya, Tadashi R
AU  - Miya TR
AD  - Department of Ophthalmology and Visual Science, John A Moran Eye Center, 
      University of Utah, Salt Lake City, UT 84109, United States.
FAU - Bankhead, Peter
AU  - Bankhead P
AD  - Centre for Vision and Vascular Science, Queen's University of Belfast, Institute 
      of Clinical Sciences, Block A, Royal Victoria Hospital Belfast, Ireland.
FAU - McGeown, J Graham
AU  - McGeown JG
AD  - Centre for Vision and Vascular Science, Queen's University of Belfast, Institute 
      of Clinical Sciences, Block A, Royal Victoria Hospital Belfast, Ireland.
FAU - Curtis, Timothy M
AU  - Curtis TM
AD  - Centre for Vision and Vascular Science, Queen's University of Belfast, Institute 
      of Clinical Sciences, Block A, Royal Victoria Hospital Belfast, Ireland.
FAU - Ambati, Balamurali K
AU  - Ambati BK
AD  - Department of Ophthalmology and Visual Science, John A Moran Eye Center, 
      University of Utah, Salt Lake City, UT 84109, United States.
LA  - eng
GR  - P30 EY014800/EY/NEI NIH HHS/United States
GR  - R01 EY017182/EY/NEI NIH HHS/United States
GR  - R01 EY017950/EY/NEI NIH HHS/United States
GR  - T32 DK091317/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131212
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - *Acridine Orange
MH  - Animals
MH  - Blood Flow Velocity
MH  - Cell Movement/physiology
MH  - Diabetes Mellitus, Type 1/physiopathology
MH  - *Fluorescein Angiography
MH  - *Fluorescent Dyes
MH  - Leukocytes/*physiology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Inbred NOD
MH  - Microscopy, Confocal
MH  - Ophthalmoscopes
MH  - Regional Blood Flow/physiology
MH  - Retinal Artery/*physiology
MH  - Retinal Vasculitis/chemically induced/physiopathology
MH  - Retinal Vein/*physiology
MH  - Tomography, Optical Coherence
MH  - Vascular Endothelial Growth Factor A/pharmacology
MH  - Video Recording
PMC - PMC3943592
MID - NIHMS550536
OTO - NOTNLM
OT  - acridine orange
OT  - blood flow
OT  - inflammation
OT  - leukocyte
OT  - retina
EDAT- 2013/12/18 06:00
MHDA- 2014/05/03 06:00
PMCR- 2015/03/01
CRDT- 2013/12/17 06:00
PHST- 2013/05/29 00:00 [received]
PHST- 2013/11/25 00:00 [revised]
PHST- 2013/12/04 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/05/03 06:00 [medline]
PHST- 2015/03/01 00:00 [pmc-release]
AID - S0014-4835(13)00346-1 [pii]
AID - 10.1016/j.exer.2013.12.002 [doi]
PST - ppublish
SO  - Exp Eye Res. 2014 Mar;120:15-9. doi: 10.1016/j.exer.2013.12.002. Epub 2013 Dec 
      12.