PMID- 24336154
OWN - NLM
STAT- MEDLINE
DCOM- 20150221
LR  - 20230210
IS  - 1530-0285 (Electronic)
IS  - 0893-3952 (Linking)
VI  - 27
IP  - 7
DP  - 2014 Jul
TI  - Lack of presence of the human cytomegalovirus in human glioblastoma.
PG  - 922-9
LID - 10.1038/modpathol.2013.219 [doi]
AB  - Recent reports have indicated human cytomegalovirus (HCMV) to be associated with 
      human glioblastoma carcinogenesis. In established examples of viral 
      carcinogenesis, viral DNA and one or more of its products have been detected in 
      most tumor cells of biopsies in the majority of cases. To test whether HCMV is 
      associated with human glioblastoma based on this criterion, we measured the 
      number of viral DNA molecules per cell in both frozen and paraffin-embedded tumor 
      biopsies from 58 patients using real-time quantitative PCR (QPCR). 
      Immunohistochemical and fluorescence in situ hybridization (FISH) to detect HCMV 
      proteins and genome was performed in 10 cases using formalin-fixed 
      paraffin-embedded glioblastoma tissues. Southern blotting using DNA extracted 
      from four glioblastoma cell lines together with immunoblotting using the four 
      cell lines and five glioblastoma tissue samples were also performed. We further 
      confirmed the immunoblot bands using liquid chromatography-tandem mass 
      spectrometry assay. As a result, HCMV DNA was not detected in the tumor cells 
      from any of the glioblastoma cases by QPCR detecting two different HCMV genes, in 
      clear contrast to samples from patients with HCMV infection. Southern blotting 
      and immunoblotting of cell lines and FISH using paraffin sections were all 
      negative. However, immunoblotting and immunohistochemistry using tissue samples 
      were partly positive, but HCMV proteins were not detected by proteomic analysis, 
      suggesting false positivity of the analyses. As our QPCR analysis could detect 10 
      copies of HCMV DNA mixed with DNA extracted from 10(4) HCMV-negative cells, we 
      conclude that HCMV is not persistent, at least in the tumor cells, of developed 
      human glioblastoma.
FAU - Yamashita, Yoriko
AU  - Yamashita Y
AD  - 1] Department of Experimental Pathology and Tumor Biology, Nagoya City University 
      Graduate School of Medical Sciences, Nagoya, Japan [2] Department of Pathology 
      and Biological Responses, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
FAU - Ito, Yoshinori
AU  - Ito Y
AD  - Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, 
      Japan.
FAU - Isomura, Hiroki
AU  - Isomura H
AD  - Department of Virology and Preventive Medicine, Gunma University Graduate School 
      of Medicine, Maebashi, Gunma, Japan.
FAU - Takemura, Naoaki
AU  - Takemura N
AD  - Department of Pathology and Biological Responses, Nagoya University Graduate 
      School of Medicine, Nagoya, Japan.
FAU - Okamoto, Akira
AU  - Okamoto A
AD  - Department of School Nursing and Health Education, Aichi University of Education, 
      Kariya, Aichi, Japan.
FAU - Motomura, Kazuya
AU  - Motomura K
AD  - Department of Neurosurgery, Nagoya University Graduate School of Medicine, 
      Nagoya, Japan.
FAU - Tsujiuchi, Takashi
AU  - Tsujiuchi T
AD  - Department of Neurosurgery, Nagoya University Graduate School of Medicine, 
      Nagoya, Japan.
FAU - Natsume, Atsushi
AU  - Natsume A
AD  - Department of Neurosurgery, Nagoya University Graduate School of Medicine, 
      Nagoya, Japan.
FAU - Wakabayashi, Toshihiko
AU  - Wakabayashi T
AD  - Department of Neurosurgery, Nagoya University Graduate School of Medicine, 
      Nagoya, Japan.
FAU - Toyokuni, Shinya
AU  - Toyokuni S
AD  - Department of Pathology and Biological Responses, Nagoya University Graduate 
      School of Medicine, Nagoya, Japan.
FAU - Tsurumi, Tatsuya
AU  - Tsurumi T
AD  - Division of Virology, Aichi Cancer Center Research Institute, Nagoya, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131213
PL  - United States
TA  - Mod Pathol
JT  - Modern pathology : an official journal of the United States and Canadian Academy 
      of Pathology, Inc
JID - 8806605
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Brain Neoplasms/pathology/*virology
MH  - Child
MH  - Cytomegalovirus/*isolation & purification
MH  - DNA, Viral/*analysis
MH  - Female
MH  - Glioblastoma/pathology/*virology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Middle Aged
MH  - Real-Time Polymerase Chain Reaction
EDAT- 2013/12/18 06:00
MHDA- 2015/02/24 06:00
CRDT- 2013/12/17 06:00
PHST- 2013/08/21 00:00 [received]
PHST- 2013/11/02 00:00 [revised]
PHST- 2013/11/12 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2015/02/24 06:00 [medline]
AID - S0893-3952(22)01875-0 [pii]
AID - 10.1038/modpathol.2013.219 [doi]
PST - ppublish
SO  - Mod Pathol. 2014 Jul;27(7):922-9. doi: 10.1038/modpathol.2013.219. Epub 2013 Dec 
      13.