PMID- 24337259
OWN - NLM
STAT- MEDLINE
DCOM- 20140826
LR  - 20201209
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 9
IP  - 2
DP  - 2014 Feb
TI  - Suppression of 2,4-dinitrochlorobenzene-induced atopic dermatitis by extract of 
      Bacillus Calmette-Guerin.
PG  - 689-94
LID - 10.3892/mmr.2013.1865 [doi]
AB  - Bacillus Calmette-Guerin extract (BCGE) has been proven to be clinically 
      effective for anaphylactic disease, infectious diseases and cancer. In this 
      study, we investigated the effect of the intramuscular application of BCGE on 
      2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD). We established an 
      AD model in BALB/c mice by repeated local exposure of DNCB to the ear and dorsal 
      skin. Following intramuscular application of BCGE, the ear thickness, mast cell 
      infiltration, serum total immunoglobulin E (IgE) and histamine level were 
      measured. In addition, levels of interleukin (IL)-4, IL-13, interferon (IFN)-gamma 
      and tumor necrosis factor (TNF)-alpha in the ears were assayed. BCGE reduced AD 
      symptoms based on ear thickness, dermatitis score, histopathological analysis and 
      serum IgE levels. In addition, BCGE inhibited mast cell infiltration into the ear 
      and elevation of serum histamine, increased IFN-gamma level and suppressed IL-4, 
      IL-13 and TNF-alpha levels in the ears. Furthermore, BCGE attenuated the NF-kappaBp65 
      expression in the nuclear extract of the ear tissue. Taken together, our results 
      demonstrated that intramuscular application of BCGE exerts beneficial effects on 
      the symptoms of AD suggesting that BCGE may be a candidate for the treatment of 
      AD.
FAU - Sun, Mingli
AU  - Sun M
AD  - Department of Pharmacology, College of Pharmaceutical Science, China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Wang, Shuang
AU  - Wang S
AD  - Department of Pharmacology, College of Pharmaceutical Science, China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Zhao, Lin
AU  - Zhao L
AD  - Department of Pharmacology, College of Pharmaceutical Science, China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Zhao, Haishan
AU  - Zhao H
AD  - Department of Pharmacology, College of Pharmaceutical Science, China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Yao, Weifan
AU  - Yao W
AD  - Department of Pharmacology, College of Pharmaceutical Science, China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Jin, Wanbao
AU  - Jin W
AD  - Department of Pharmacology, College of Pharmaceutical Science, China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
FAU - Wei, Minjie
AU  - Wei M
AD  - Department of Pharmacology, College of Pharmaceutical Science, China Medical 
      University, Shenyang, Liaoning 110001, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131216
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (BCG Vaccine)
RN  - 0 (Cell Extracts)
RN  - 0 (Dinitrochlorobenzene)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 820484N8I3 (Histamine)
SB  - IM
MH  - Animals
MH  - BCG Vaccine/*administration & dosage
MH  - Cell Extracts/*administration & dosage
MH  - Dermatitis, Atopic/blood/chemically induced/*drug therapy/pathology
MH  - Dinitrochlorobenzene/toxicity
MH  - Disease Models, Animal
MH  - Histamine/blood
MH  - Immunoglobulin E/blood/drug effects
MH  - Mast Cells/drug effects/pathology
MH  - Mice
EDAT- 2013/12/18 06:00
MHDA- 2014/08/27 06:00
CRDT- 2013/12/17 06:00
PHST- 2013/06/06 00:00 [received]
PHST- 2013/11/28 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/08/27 06:00 [medline]
AID - 10.3892/mmr.2013.1865 [doi]
PST - ppublish
SO  - Mol Med Rep. 2014 Feb;9(2):689-94. doi: 10.3892/mmr.2013.1865. Epub 2013 Dec 16.