PMID- 24338609
OWN - NLM
STAT- MEDLINE
DCOM- 20140401
LR  - 20140210
IS  - 1554-527X (Electronic)
IS  - 0736-0266 (Linking)
VI  - 32
IP  - 4
DP  - 2014 Apr
TI  - Anti-inflammatory effect of platelet-rich plasma on nucleus pulposus cells with 
      response of TNF-alpha and IL-1.
PG  - 551-6
LID - 10.1002/jor.22532 [doi]
AB  - The purpose of this study was to investigate the anti-inflammatory effect of 
      platelet-rich plasma (PRP) with collagen matrix on human nucleus pulposus (NP) 
      cell in response to pro-inflammatory cytokines such as tumor necrosis 
      factor-alpha (TNF-alpha) and interleukin-1 (IL-1). NP cells from human disks were 
      cultured in a monolayer and maintained in the collagen matrix prior to the 
      addition of recombinant human IL-1 and TNF-alpha. After applying IL-1 and TNF-alpha, PRP 
      prepared by using a commercially available platelet concentration system was 
      added. The response was investigated using real-time PCR for mRNA expression of 
      type II collagen, aggrecan, matrix metalloproteinase-3 (MMP-3), and 
      cyclooxygenase-2 (COX-2). The combination of IL-1beta and TNF-alpha led to decrease of 
      matrix synthesis gene expression such as collagen type II and aggrecan and 
      increase of the degradation gene expression of COX-2 and MMP-3, compared to the 
      control. Consecutive PRP exposure significantly recovered the down-regulated gene 
      expression of collagen type II and aggrecan and significantly reduced the 
      increased MMP-3 and COX-2 gene expression, compared to that of control groups 
      with pro-inflammatory cytokines. The administration of PRP with collagen matrix 
      markedly suppressed cytokine-induced pro-inflammatory degrading enzymes and 
      mediators in the NP cell. It also rescued gene expression concerning matrix 
      synthesis, thereby stabilizing NP cell differentiation.
CI  - (c) 2013 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
FAU - Kim, Ho-Joong
AU  - Kim HJ
AD  - Department of Orthopaedic Surgery, Seoul National University College of Medicine, 
      166 Gumiro, Bundang-gu, Sungnam, 463-707, Republic of Korea; Spine Center, Seoul 
      National University Bundang Hospital, 166 Gumiro, Bundang-gu, Sungnam, 463-707, 
      Republic of Korea.
FAU - Yeom, Jin S
AU  - Yeom JS
FAU - Koh, Yong-Gon
AU  - Koh YG
FAU - Yeo, Jee-Eun
AU  - Yeo JE
FAU - Kang, Kyoung-Tak
AU  - Kang KT
FAU - Kang, Young-Mi
AU  - Kang YM
FAU - Chang, Bong-Soon
AU  - Chang BS
FAU - Lee, Choon-Ki
AU  - Lee CK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131211
PL  - United States
TA  - J Orthop Res
JT  - Journal of orthopaedic research : official publication of the Orthopaedic 
      Research Society
JID - 8404726
RN  - 0 (Collagen Type II)
RN  - 0 (Interleukin-1beta)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
RN  - EC 1.14.99.1 (PTGS2 protein, human)
RN  - EC 3.4.24.17 (MMP3 protein, human)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Collagen Type II/antagonists & inhibitors/biosynthesis
MH  - Cyclooxygenase 2/biosynthesis/metabolism
MH  - Down-Regulation/physiology
MH  - Healthy Volunteers
MH  - Humans
MH  - Interleukin-1beta/*physiology
MH  - Intervertebral Disc Degeneration/*metabolism/pathology/*therapy
MH  - Matrix Metalloproteinase 3/biosynthesis/metabolism
MH  - Middle Aged
MH  - *Platelet-Rich Plasma/chemistry/cytology
MH  - Tumor Necrosis Factor-alpha/*physiology
OTO - NOTNLM
OT  - interleukin-1
OT  - nucleus pulposus cell
OT  - platelet-rich plasma
OT  - tumor necrosis factor-alpha
EDAT- 2013/12/18 06:00
MHDA- 2014/04/02 06:00
CRDT- 2013/12/17 06:00
PHST- 2013/08/25 00:00 [received]
PHST- 2013/11/09 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/04/02 06:00 [medline]
AID - 10.1002/jor.22532 [doi]
PST - ppublish
SO  - J Orthop Res. 2014 Apr;32(4):551-6. doi: 10.1002/jor.22532. Epub 2013 Dec 11.