PMID- 24339206
OWN - NLM
STAT- MEDLINE
DCOM- 20141002
LR  - 20140115
IS  - 1098-2264 (Electronic)
IS  - 1045-2257 (Linking)
VI  - 53
IP  - 3
DP  - 2014 Mar
TI  - Microfluidic channel-assisted screening of hematopoietic malignancies.
PG  - 255-63
LID - 10.1002/gcc.22137 [doi]
AB  - A simple microfluidic fluorescence in situ hybridization (FISH) device allowing 
      accurate analysis of interphase nuclei in 1 hr in narrow channels is presented. 
      Photolithography and fluorosilicic acid etching were used to fabricate 
      microfluidic channels (referred to as FISHing lines) that allowed analysis of 10 
      samples on a glass microscope slide 0.2 microl of sample volume was used to fill a 
      micro-channel, which resembled a 250-fold reduction compared to conventional 
      FISH. FISH signals were comparable to conventional FISH, with 50-fold less probe 
      consumption and 10-fold less time. Cells were immobilized in single file in 
      channels just exceeding the diameter of the cells, and were used for minimal 
      residual disease (MRD) analysis. To test the micro-channels for application in 
      FISH, MRD was simulated by mixing K562 cells (an established chronic myeloid 
      leukemia cell line) carrying the BCR/ABL fusion gene across 1:1 to 1:1,000 Jurkat 
      cells (an established acute lymphoblastic leukemia cell line). The limit of 
      detection was seen to be 1:100 cells and 1:1,000 cells for FISHing lines and 
      conventional FISH, respectively; however, the conventional method seemed to 
      over-score the presence of K562 cells. This may in part be attributed to FISHing 
      lines practically eliminating the chance of duplicate screening of cells and 
      hastened the time of screening, enhancing scoring of all cells within the 
      channels. This was compared to 1 in 500 cells on the slide being analyzed with 
      the conventional FISH.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Mughal, Farah
AU  - Mughal F
AD  - Manchester Interdisciplinary Biocentre, University of Manchester, Manchester, M1, 
      7ND, UK.
FAU - Baldock, Sara J
AU  - Baldock SJ
FAU - Karimiani, Ehsan Ghayoor
AU  - Karimiani EG
FAU - Telford, Nick
AU  - Telford N
FAU - Goddard, Nicholas J
AU  - Goddard NJ
FAU - Day, Philip J R
AU  - Day PJ
LA  - eng
PT  - Journal Article
DEP - 20131216
PL  - United States
TA  - Genes Chromosomes Cancer
JT  - Genes, chromosomes & cancer
JID - 9007329
SB  - IM
MH  - Cell Size
MH  - Hematologic Neoplasms/*diagnosis/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Jurkat Cells
MH  - K562 Cells
MH  - Microfluidics
MH  - Neoplasm, Residual
EDAT- 2013/12/18 06:00
MHDA- 2014/10/03 06:00
CRDT- 2013/12/17 06:00
PHST- 2013/09/03 00:00 [received]
PHST- 2013/12/03 00:00 [accepted]
PHST- 2013/12/17 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/10/03 06:00 [medline]
AID - 10.1002/gcc.22137 [doi]
PST - ppublish
SO  - Genes Chromosomes Cancer. 2014 Mar;53(3):255-63. doi: 10.1002/gcc.22137. Epub 
      2013 Dec 16.