PMID- 24341374 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20140206 LR - 20240323 IS - 1755-8166 (Print) IS - 1755-8166 (Electronic) IS - 1755-8166 (Linking) VI - 6 IP - 1 DP - 2013 Dec 17 TI - First detailed reconstruction of the karyotype of Trachypithecus cristatus (Mammalia: Cercopithecidae). PG - 58 LID - 10.1186/1755-8166-6-58 [doi] AB - BACKGROUND: The chromosomal homologies of human (Homo sapiens = HSA) and silvered leaf monkey (Trachypithecus cristatus = TCR) have been previously studied by classical chromosome staining and by fluorescence in situ hybridization (FISH) applying chromosome-specific DNA probes of all human chromosomes in the 1980s and 1990s, respectively. RESULTS: However, as the resolution of these techniques is limited we used multicolor banding (MCB) at an ~250-band level, and other selected human DNA probes to establish a detailed chromosomal map of TCR. Therefore it was possible to precisely determine evolutionary conserved breakpoints, orientation of segments and distribution of specific regions in TCR compared to HSA. Overall, 69 evolutionary conserved breakpoints including chromosomal segments, which failed to be resolved in previous reports, were exactly identified and characterized. CONCLUSIONS: This work also represents the first molecular cytogenetic one characterizing a multiple sex chromosome system with a male karyotype 44,XY1Y2. The obtained results are compared to other available data for old world monkeys and drawbacks in hominoid evolution are discussed. FAU - Xiaobo, Fan AU - Xiaobo F FAU - Pinthong, Krit AU - Pinthong K FAU - Mkrtchyan, Hasmik AU - Mkrtchyan H FAU - Siripiyasing, Pornnarong AU - Siripiyasing P FAU - Kosyakova, Nadezda AU - Kosyakova N FAU - Supiwong, Weerayuth AU - Supiwong W FAU - Tanomtong, Alongkoad AU - Tanomtong A FAU - Chaveerach, Arunrat AU - Chaveerach A FAU - Liehr, Thomas AU - Liehr T FAU - de Bello Cioffi, Marcelo AU - de Bello Cioffi M FAU - Weise, Anja AU - Weise A AD - Institute of Human Genetics, Jena University Hospital, Friedrich Schiller University, Kollegiengasse 10, Jena D-07743, Germany. Anja.Weise@med.uni-jena.de. LA - eng PT - Journal Article DEP - 20131217 PL - England TA - Mol Cytogenet JT - Molecular cytogenetics JID - 101317942 PMC - PMC3914712 EDAT- 2013/12/18 06:00 MHDA- 2013/12/18 06:01 PMCR- 2013/12/17 CRDT- 2013/12/18 06:00 PHST- 2013/11/15 00:00 [received] PHST- 2013/11/21 00:00 [accepted] PHST- 2013/12/18 06:00 [entrez] PHST- 2013/12/18 06:00 [pubmed] PHST- 2013/12/18 06:01 [medline] PHST- 2013/12/17 00:00 [pmc-release] AID - 1755-8166-6-58 [pii] AID - 10.1186/1755-8166-6-58 [doi] PST - epublish SO - Mol Cytogenet. 2013 Dec 17;6(1):58. doi: 10.1186/1755-8166-6-58.