PMID- 24343717
OWN - NLM
STAT- MEDLINE
DCOM- 20150516
LR  - 20211203
IS  - 0371-0874 (Print)
IS  - 0371-0874 (Linking)
VI  - 65
IP  - 6
DP  - 2013 Dec 25
TI  - [Palmitate induces apoptosis and endoplasmic reticulum stress in human umbilical 
      cord-derived mesenchymal stem cells].
PG  - 600-6
AB  - The saturated free fatty acid (FFA), palmitate, could induce apoptosis in various 
      cell types, but little is known about its effects on human umbilical cord-derived 
      mesenchymal stem cells (hUC-MSCs). Here, we investigated whether palmitate 
      induced apoptosis and endoplasmic reticulum (ER) stress in hUC-MSCs. hUC-MSCs 
      were stained by labeled antibodies and identified by flow cytometry. After 
      administration with palmitate, apoptotic cell was assessed by flow cytometry 
      using the Annexin V-FITC/7-AAD apoptosis detection kit. Relative spliced XBP1 
      levels were analyzed using semi-quantitative RT-PCR. The mRNA of BiP, GRP94, ATF4 
      and CHOP were analyzed by real-time PCR. Relative BiP and CHOP protein were 
      analyzed using Western blot analysis. The results showed that hUC-MSCs were 
      homogeneously positive for MSC markers; palmitate increased apoptosis of hUC-MSCs 
      and activated XBP1 splicing, BiP, GRP94, ATF4 and CHOP transcription. These 
      findings suggest that palmitate induces apoptosis and ER stress in hUC-MSCs.
FAU - Lu, Jun
AU  - Lu J
AD  - Fujian Provincial Key Laboratory of Transplant Biology, Fuzhou General Hospital, 
      Fuzhou 350025, China. hlhtiger@163.com, tanjm156@gmail.com.
FAU - Dong, Hui-Yue
AU  - Dong HY
FAU - Lin, Ling-Jing
AU  - Lin LJ
FAU - Wang, Qing-Hua
AU  - Wang QH
FAU - Huang, Liang-Hu
AU  - Huang LH
FAU - Tan, Jian-Ming
AU  - Tan JM
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Sheng Li Xue Bao
JT  - Sheng li xue bao : [Acta physiologica Sinica]
JID - 20730130R
RN  - 0 (ATF4 protein, human)
RN  - 0 (DDIT3 protein, human)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Palmitates)
RN  - 0 (Regulatory Factor X Transcription Factors)
RN  - 0 (Transcription Factors)
RN  - 0 (X-Box Binding Protein 1)
RN  - 0 (XBP1 protein, human)
RN  - 0 (endoplasmin)
RN  - 145891-90-3 (Activating Transcription Factor 4)
RN  - 147336-12-7 (Transcription Factor CHOP)
SB  - IM
MH  - Activating Transcription Factor 4/metabolism
MH  - *Apoptosis
MH  - DNA-Binding Proteins/metabolism
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - *Endoplasmic Reticulum Stress
MH  - Heat-Shock Proteins/metabolism
MH  - Humans
MH  - Membrane Glycoproteins/metabolism
MH  - Mesenchymal Stem Cells/cytology/*drug effects
MH  - Palmitates/*pharmacology
MH  - Regulatory Factor X Transcription Factors
MH  - Transcription Factor CHOP/metabolism
MH  - Transcription Factors/metabolism
MH  - Umbilical Cord/cytology
MH  - X-Box Binding Protein 1
EDAT- 2013/12/18 06:00
MHDA- 2015/05/20 06:00
CRDT- 2013/12/18 06:00
PHST- 2013/12/18 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2015/05/20 06:00 [medline]
PST - ppublish
SO  - Sheng Li Xue Bao. 2013 Dec 25;65(6):600-6.