PMID- 24343743
OWN - NLM
STAT- MEDLINE
DCOM- 20140224
LR  - 20141120
IS  - 1943-7722 (Electronic)
IS  - 0002-9173 (Linking)
VI  - 141
IP  - 1
DP  - 2014 Jan
TI  - Bright-field HER2 dual in situ hybridization (DISH) assay vs fluorescence in situ 
      hybridization (FISH): focused study of immunohistochemical 2+ cases.
PG  - 102-10
LID - 10.1309/AJCP6CXS8OSRHXIR [doi]
AB  - OBJECTIVES: To compare the INFORM HER2 bright-field dual in situ hybridization 
      (DISH) DNA probe cocktail assay with the PathVysion fluorescence in situ 
      hybridization (FISH) assay on 103 invasive breast carcinomas with a 2+ score on 
      immunohistochemistry (IHC). METHODS: The cases were categorized as positive, 
      equivocal, or negative for HER2 gene amplification using the 2007 American 
      Society of Clinical Oncology/College of American Pathologists (ASCO/CAP) 
      HER2:CEP17 ratio criteria and also based on mean HER2 gene copies/cell. The third 
      criterion used a HER2:CEP17 ratio of 2 to categorize cases as positive or 
      negative. RESULTS: The agreement between FISH and DISH was 85% using the 2007 
      ASCO/CAP ratio criterion, 79% using the mean HER2 gene copies/cell criterion, and 
      92% using the 2.0 cutoff HER2:CEP17 ratio criterion. In addition, 20 known IHC 3+ 
      breast carcinomas analyzed by DISH showed clusters of the HER2 gene consistent 
      with unequivocal amplification. CONCLUSIONS: Despite some technical and 
      interpretational issues associated with DISH, it compares favorably with FISH in 
      this group of challenging breast cancer cases.
FAU - Gao, Faye F
AU  - Gao FF
AD  - Magee-Womens Hospital of University of Pittsburgh Medical Center, 300 Halket St, 
      Pittsburgh, PA 15213; rbhargava@mail.magee.edu.
FAU - Dabbs, David J
AU  - Dabbs DJ
FAU - Cooper, Kristine L
AU  - Cooper KL
FAU - Bhargava, Rohit
AU  - Bhargava R
LA  - eng
GR  - P30CA047904/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - England
TA  - Am J Clin Pathol
JT  - American journal of clinical pathology
JID - 0370470
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*genetics/pathology
MH  - Chromosomes, Human, Pair 17/ultrastructure
MH  - Female
MH  - *Genes, erbB-2
MH  - Humans
MH  - Immunohistochemistry
MH  - In Situ Hybridization/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Receptor, ErbB-2/*biosynthesis
OTO - NOTNLM
OT  - DISH
OT  - FISH
OT  - HER2 gene
OT  - HER2 immunohistochemistry
OT  - IHC score 2+
EDAT- 2013/12/18 06:00
MHDA- 2014/02/25 06:00
CRDT- 2013/12/18 06:00
PHST- 2013/12/18 06:00 [entrez]
PHST- 2013/12/18 06:00 [pubmed]
PHST- 2014/02/25 06:00 [medline]
AID - 141/1/102 [pii]
AID - 10.1309/AJCP6CXS8OSRHXIR [doi]
PST - ppublish
SO  - Am J Clin Pathol. 2014 Jan;141(1):102-10. doi: 10.1309/AJCP6CXS8OSRHXIR.