PMID- 24344904
OWN - NLM
STAT- MEDLINE
DCOM- 20150515
LR  - 20181202
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 23
IP  - 18
DP  - 2014 Sep 15
TI  - Plasma elevation of vascular endothelial growth factor leads to the reduction of 
      mouse hematopoietic and mesenchymal stem/progenitor cells in the bone marrow.
PG  - 2202-10
LID - 10.1089/scd.2013.0469 [doi]
AB  - Vascular endothelial growth factor (VEGF) is reported to exhibit potent 
      hematopoietic stem/progenitor cell (HSPC) mobilization activity. However, the 
      detailed mechanisms of HSPC mobilization by VEGF have not been examined. In this 
      study, we investigated the effect of VEGF on bone marrow (BM) cell and the BM 
      environment by intravenous injection of VEGF-expressing adenovirus vector 
      (Ad-VEGF) into mice. A colony assay using peripheral blood cells revealed that 
      plasma elevation of VEGF leads to the mobilization of HSPCs into the circulation. 
      Granulocyte colony-stimulating factor (G-CSF) is known to mobilize HSPCs by 
      decreasing CXC chemokine ligand 12 (CXCL12) levels in the BM. However, we found 
      almost no changes in the CXCL12 levels in the BM after Ad-VEGF injection, 
      suggesting that VEGF can alter the BM microenvironment by different mechanisms 
      from G-CSF. Furthermore, flow cytometric analysis and colony forming 
      unit-fibroblast assay showed a reduction in the number of mesenchymal progenitor 
      cells (MPCs), which have been reported to serve as niche cells to support HSPCs, 
      in the BM of Ad-VEGF-injected mice. Adhesion of donor cells to the recipient BM 
      after transplantation was also impaired in mice injected with Ad-VEGF, suggesting 
      a decrease in the niche cell number. We also observed a dose-dependent 
      chemoattractive effect of VEGF on primary BM stromal cells in vitro. These data 
      suggest that VEGF alters the distribution of MPCs in the BM and can also mobilize 
      MPCs to peripheral tissues. Taken together, our results imply that VEGF-elicited 
      egress of HSPCs would be mediated, in part, by changing the number of MPCs in the 
      BM.
FAU - Tashiro, Katsuhisa
AU  - Tashiro K
AD  - 1 Laboratory of Stem Cell Regulation, National Institute of Biomedical Innovation 
      , Osaka, Japan .
FAU - Nonaka, Aki
AU  - Nonaka A
FAU - Hirata, Nobue
AU  - Hirata N
FAU - Yamaguchi, Tomoko
AU  - Yamaguchi T
FAU - Mizuguchi, Hiroyuki
AU  - Mizuguchi H
FAU - Kawabata, Kenji
AU  - Kawabata K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20140131
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adenoviridae/metabolism
MH  - Animals
MH  - Bone Marrow/*metabolism
MH  - Cell Count
MH  - Cell Movement
MH  - Chemokine CXCL12/metabolism
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/metabolism
MH  - Mice
MH  - Vascular Endothelial Growth Factor A/*blood
EDAT- 2013/12/19 06:00
MHDA- 2015/05/16 06:00
CRDT- 2013/12/19 06:00
PHST- 2013/12/19 06:00 [entrez]
PHST- 2013/12/19 06:00 [pubmed]
PHST- 2015/05/16 06:00 [medline]
AID - 10.1089/scd.2013.0469 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2014 Sep 15;23(18):2202-10. doi: 10.1089/scd.2013.0469. Epub 2014 
      Jan 31.