PMID- 24345682
OWN - NLM
STAT- MEDLINE
DCOM- 20140808
LR  - 20220129
IS  - 1937-9145 (Electronic)
IS  - 1945-0877 (Print)
IS  - 1945-0877 (Linking)
VI  - 6
IP  - 306
DP  - 2013 Dec 17
TI  - Deep sequencing and high-resolution imaging reveal compartment-specific 
      localization of Bdnf mRNA in hippocampal neurons.
PG  - rs16
LID - 10.1126/scisignal.2004520 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) is a small protein of the neurotrophin 
      family that regulates various brain functions. Although much is known about how 
      its transcription is regulated, the abundance of endogenous BDNF mRNA and its 
      subcellular localization pattern are matters of debate. We used next-generation 
      sequencing and high-resolution in situ hybridization in the rat hippocampus to 
      reexamine this question. We performed 3' end sequencing on rat hippocampal slices 
      and detected two isoforms of Bdnf containing either a short or a long 3' 
      untranslated region (3'UTR). Most of the Bdnf transcripts contained the short 
      3'UTR isoform and were present in low amounts relative to other neuronal 
      transcripts. Bdnf mRNA was present in the somatic compartment of rat hippocampal 
      slices or the somata of cultured rat hippocampal neurons but was rarely detected 
      in the dendritic processes. Pharmacological stimulation of hippocampal neurons 
      induced Bdnf expression but did not change the ratio of Bdnf isoform abundance. 
      The findings indicate that endogenous Bdnf mRNA, although weakly abundant, is 
      primarily localized to the somatic compartment of hippocampal neurons. Both Bdnf 
      mRNA isoforms have shorter half-lives compared with other neuronal mRNAs. 
      Furthermore, the findings show that using complementary high-resolution 
      techniques can provide sensitive measures of endogenous transcript abundance.
FAU - Will, Tristan J
AU  - Will TJ
AD  - Department of Synaptic Plasticity, Max Planck Institute for Brain Research, Max 
      von Laue Strasse 4, 60438 Frankfurt, Germany.
FAU - Tushev, Georgi
AU  - Tushev G
FAU - Kochen, Lisa
AU  - Kochen L
FAU - Nassim-Assir, Belquis
AU  - Nassim-Assir B
FAU - Cajigas, Ivan J
AU  - Cajigas IJ
FAU - Tom Dieck, Susanne
AU  - Tom Dieck S
FAU - Schuman, Erin M
AU  - Schuman EM
LA  - eng
GR  - 294376/ERC_/European Research Council/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131217
PL  - United States
TA  - Sci Signal
JT  - Science signaling
JID - 101465400
RN  - 0 (3' Untranslated Regions)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*genetics
MH  - *High-Throughput Nucleotide Sequencing
MH  - Hippocampus/cytology/*metabolism
MH  - In Situ Hybridization
MH  - Neurons/*metabolism
MH  - RNA, Messenger/*genetics
MH  - Rats
PMC - PMC5321484
MID - EMS68492
COIS- Competing interests: The authors declare that they have no competing interests.
EDAT- 2013/12/19 06:00
MHDA- 2014/08/13 06:00
PMCR- 2017/02/22
CRDT- 2013/12/19 06:00
PHST- 2013/12/19 06:00 [entrez]
PHST- 2013/12/19 06:00 [pubmed]
PHST- 2014/08/13 06:00 [medline]
PHST- 2017/02/22 00:00 [pmc-release]
AID - 6/306/rs16 [pii]
AID - 10.1126/scisignal.2004520 [doi]
PST - epublish
SO  - Sci Signal. 2013 Dec 17;6(306):rs16. doi: 10.1126/scisignal.2004520.