PMID- 24346263
OWN - NLM
STAT- MEDLINE
DCOM- 20150202
LR  - 20211021
IS  - 1432-119X (Electronic)
IS  - 0948-6143 (Linking)
VI  - 141
IP  - 5
DP  - 2014 May
TI  - Subcellular localization of NAPE-PLD and DAGL-alpha in the ventromedial nucleus of 
      the hypothalamus by a preembedding immunogold method.
PG  - 543-50
LID - 10.1007/s00418-013-1174-x [doi]
AB  - The hypothalamus and the endocannabinoid system are important players in the 
      regulation of energy homeostasis. In a previous study, we described the 
      ultrastructural distribution of CB1 receptors in GABAergic and glutamatergic 
      synaptic terminals of the dorsomedial region of the ventromedial nucleus of the 
      hypothalamus (VMH). However, the specific localization of the enzymes responsible 
      for the synthesis of the two main endocannabinoids in the hypothalamus is not 
      known. The objective of this study was to investigate the precise subcellular 
      distribution of N-arachidonoylphospatidylethanolamine phospholipase D (NAPE-PLD) 
      and diacylglycerol lipase alpha (DAGL-alpha) in the dorsomedial VMH of wild-type mice by 
      a high resolution immunogold electron microscopy technique. Knock-out mice for 
      each enzyme were used to validate the specificity of the antibodies. NAPE-PLD was 
      localized presynaptically and postsynaptically but showed a preferential 
      distribution in dendrites. DAGL-alpha was mostly postsynaptic in dendrites and 
      dendritic spines. These anatomical results contribute to a better understanding 
      of the endocannabinoid modulation in the VMH nucleus. Furthermore, they support 
      the idea that the dorsomedial VMH displays the necessary machinery for the 
      endocannabinoid-mediated modulation of synaptic transmission of brain circuitries 
      that regulate important hypothalamic functions such as feeding behaviors.
FAU - Reguero, Leire
AU  - Reguero L
AD  - Department of Neurosciences, Faculty of Medicine and Dentistry, University of the 
      Basque Country UPV/EHU, 48940, Leioa, Vizcaya, Spain.
FAU - Puente, Nagore
AU  - Puente N
FAU - Elezgarai, Izaskun
AU  - Elezgarai I
FAU - Ramos-Uriarte, Almudena
AU  - Ramos-Uriarte A
FAU - Gerrikagoitia, Inmaculada
AU  - Gerrikagoitia I
FAU - Bueno-Lopez, Jose-Luis
AU  - Bueno-Lopez JL
FAU - Donate, Francisco
AU  - Donate F
FAU - Grandes, Pedro
AU  - Grandes P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131218
PL  - Germany
TA  - Histochem Cell Biol
JT  - Histochemistry and cell biology
JID - 9506663
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
RN  - EC 3.1.4.4 (N-acylphosphatidylethanolamine phospholipase D, mouse)
RN  - EC 3.1.4.4 (Phospholipase D)
SB  - IM
MH  - Animals
MH  - Female
MH  - *Immunohistochemistry
MH  - Lipoprotein Lipase/*analysis/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Microscopy, Electron
MH  - Phospholipase D/*analysis/metabolism
MH  - Tissue Embedding
MH  - Ventromedial Hypothalamic Nucleus/*enzymology/ultrastructure
EDAT- 2013/12/19 06:00
MHDA- 2015/02/03 06:00
CRDT- 2013/12/19 06:00
PHST- 2013/12/05 00:00 [accepted]
PHST- 2013/12/19 06:00 [entrez]
PHST- 2013/12/19 06:00 [pubmed]
PHST- 2015/02/03 06:00 [medline]
AID - 10.1007/s00418-013-1174-x [doi]
PST - ppublish
SO  - Histochem Cell Biol. 2014 May;141(5):543-50. doi: 10.1007/s00418-013-1174-x. Epub 
      2013 Dec 18.