PMID- 24347047
OWN - NLM
STAT- MEDLINE
DCOM- 20140404
LR  - 20161125
IS  - 1098-2280 (Electronic)
IS  - 0893-6692 (Linking)
VI  - 55
IP  - 2
DP  - 2014 Mar
TI  - Effects of silver nanoparticles on oxidative DNA damage-repair as a function of 
      p38 MAPK status: a comparative approach using human Jurkat T cells and the 
      nematode Caenorhabditis elegans.
PG  - 122-33
LID - 10.1002/em.21844 [doi]
AB  - The large-scale use of silver nanoparticles (AgNPs) has raised concerns over 
      potential impacts on the environment and human health. We previously reported 
      that AgNP exposure causes an increase in reactive oxygen species, DNA damage, and 
      induction of p38 MAPK and PMK-1 in Jurkat T cells and in Caenorhabditis elegans. 
      To elucidate the underlying mechanisms of AgNP toxicity, here we evaluate the 
      effects of AgNPs on oxidative DNA damage-repair (in human and C. elegans DNA 
      glycosylases hOGG1, hNTH1, NTH-1, and 8-oxo-GTPases-hMTH1, NDX-4) and explore the 
      role of p38 MAPK and PMK-1 in this process. Our comparative approach examined 
      viability, gene expression, and enzyme activities in wild type (WT) and p38 MAPK 
      knock-down (KD) Jurkat T cells (in vitro) and in WT and pmk-1 loss-of-function 
      mutant strains of C. elegans (in vivo). The results suggest that p38 MAPK/PMK-1 
      plays protective role against AgNP-mediated toxicity, reduced viability and 
      greater accumulation of 8OHdG was observed in AgNP-treated KD cells, and in pmk-1 
      mutant worms compared with their WT counterparts, respectively. Furthermore, 
      dose-dependent alterations in hOGG1, hMTH1, and NDX-4 expression and enzyme 
      activity, and survival in ndx-4 mutant worms occurred following AgNP exposure. 
      Interestingly, the absence or depletion of p38 MAPK/PMK-1 caused impaired and 
      additive effects in AgNP-induced ndx-4(ok1003); pmk-1(RNAi) mutant survival, and 
      hOGG1 and NDX-4 expression and enzyme activity, which may lead to higher 
      accumulation of 8OHdG. Together, the results indicate that p38 MAPK/PMK-1 plays 
      an important protective role in AgNP-induced oxidative DNA damage-repair which is 
      conserved from C. elegans to humans.
CI  - Copyright (c) 2013 Wiley Periodicals, Inc.
FAU - Chatterjee, Nivedita
AU  - Chatterjee N
AD  - School of Environmental Engineering, Graduate School of Energy and Environmental 
      system Engineering, University of Seoul, 163 Siripdaero, Dongdaemun-gu, Seoul, 
      130-743, Korea.
FAU - Eom, Hyun Jeong
AU  - Eom HJ
FAU - Choi, Jinhee
AU  - Choi J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131218
PL  - United States
TA  - Environ Mol Mutagen
JT  - Environmental and molecular mutagenesis
JID - 8800109
RN  - 0 (Anti-Infective Agents)
RN  - 0 (Caenorhabditis elegans Proteins)
RN  - 3M4G523W1G (Silver)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinases)
RN  - EC 2.7.11.24 (Pmk-1 protein, C elegans)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.1.25.1 (Deoxyribonuclease (Pyrimidine Dimer))
RN  - EC 3.1.25.1 (NTHL1 protein, human)
RN  - EC 3.1.3.2 (Phosphoric Monoester Hydrolases)
RN  - EC 3.2.2.- (DNA Glycosylases)
RN  - EC 3.2.2.- (oxoguanine glycosylase 1, human)
RN  - EC 3.6.1.- (NDX-4 protein, C elegans)
RN  - EC 3.6.1.55 (8-oxodGTPase)
RN  - EC 6.5.1.- (DNA Repair Enzymes)
SB  - IM
MH  - Animals
MH  - Anti-Infective Agents/*toxicity
MH  - Caenorhabditis elegans/*enzymology
MH  - Caenorhabditis elegans Proteins/metabolism/physiology
MH  - Cell Survival
MH  - *DNA Damage
MH  - DNA Glycosylases/metabolism
MH  - DNA Repair
MH  - DNA Repair Enzymes/metabolism
MH  - Deoxyribonuclease (Pyrimidine Dimer)/genetics/metabolism
MH  - Gene Expression
MH  - Humans
MH  - Jurkat Cells
MH  - Metal Nanoparticles/*toxicity
MH  - Mitogen-Activated Protein Kinases/physiology
MH  - Oxidation-Reduction
MH  - Oxidative Stress/genetics
MH  - Phosphoric Monoester Hydrolases/metabolism
MH  - Silver/*toxicity
MH  - p38 Mitogen-Activated Protein Kinases/*physiology
OTO - NOTNLM
OT  - 8-oxo-GTPases
OT  - 8OHdG
OT  - DNA glycosylases
OT  - PMK-1
OT  - p38 MAPK
OT  - silver nanoparticles
EDAT- 2013/12/19 06:00
MHDA- 2014/04/05 06:00
CRDT- 2013/12/19 06:00
PHST- 2013/09/16 00:00 [received]
PHST- 2013/11/19 00:00 [revised]
PHST- 2013/12/01 00:00 [accepted]
PHST- 2013/12/19 06:00 [entrez]
PHST- 2013/12/19 06:00 [pubmed]
PHST- 2014/04/05 06:00 [medline]
AID - 10.1002/em.21844 [doi]
PST - ppublish
SO  - Environ Mol Mutagen. 2014 Mar;55(2):122-33. doi: 10.1002/em.21844. Epub 2013 Dec 
      18.