PMID- 24347174
OWN - NLM
STAT- MEDLINE
DCOM- 20141021
LR  - 20221207
IS  - 1573-4919 (Electronic)
IS  - 0300-8177 (Linking)
VI  - 388
IP  - 1-2
DP  - 2014 Mar
TI  - Increased circulatory levels of lipopolysaccharide (LPS) and zonulin signify 
      novel biomarkers of proinflammation in patients with type 2 diabetes.
PG  - 203-10
LID - 10.1007/s11010-013-1911-4 [doi]
AB  - Emerging data indicate that gut-derived endotoxin (metabolic endotoxemia) may 
      contribute to low-grade systemic inflammation in insulin-resistant states. 
      Specific gut bacteria seem to serve as lipopolysaccharide (LPS) sources and 
      several reports claim a role for increased intestinal permeability in the genesis 
      of metabolic disorders. Therefore, we investigated the serum levels of LPS and 
      zonulin (ZO-1, a marker of gut permeability) along with systemic levels of tumor 
      necrosis factor-alpha (TNF-alpha) and Interleukin-6 (IL-6) in patients with type 2 
      diabetes mellitus (T2DM) compared to control subjects. Study subjects were 
      recruited from the Chennai Urban Rural Epidemiology Study [CURES], Chennai, 
      India. Study group (n = 45 each) comprised of a) subjects with normal glucose 
      tolerance (NGT) and (b) patients with T2DM. LPS, ZO-1, TNF-alpha, and IL-6 levels 
      were measured by ELISA. Serum levels of LPS [p < 0.05], LPS activity [p < 0.001], 
      ZO-1 [p < 0.001], TNFalpha [p < 0.001], and IL-6 [p < 0.001] were significantly 
      increased in patients with T2DM compared to control subjects. Pearson correlation 
      analysis revealed that LPS activity was significantly and positively correlated 
      with ZO-1, fasting plasma glucose, 2 h post glucose, HbA1c, serum triglycerides, 
      TNF-alpha, IL-6, and negatively correlated with HDL cholesterol. Regression analysis 
      showed that increased LPS levels were significantly associated with type 2 
      diabetes [odds ratio (OR) 13.43, 95 % CI 1.998-18.9; p = 0.003]. In Asian Indians 
      who are considered highly insulin resistant, the circulatory LPS levels, LPS 
      activity, and ZO-1 were significantly increased in patients with type 2 diabetes 
      and showed positive correlation with inflammatory markers and poor glycemic/lipid 
      control.
FAU - Jayashree, B
AU  - Jayashree B
AD  - Centre for Biotechnology, Anna University, Chennai, 600025, India.
FAU - Bibin, Y S
AU  - Bibin YS
FAU - Prabhu, D
AU  - Prabhu D
FAU - Shanthirani, C S
AU  - Shanthirani CS
FAU - Gokulakrishnan, K
AU  - Gokulakrishnan K
FAU - Lakshmi, B S
AU  - Lakshmi BS
FAU - Mohan, V
AU  - Mohan V
FAU - Balasubramanyam, M
AU  - Balasubramanyam M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131218
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Biomarkers)
RN  - 0 (Blood Glucose)
RN  - 0 (Cholesterol, HDL)
RN  - 0 (Glycated Hemoglobin A)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (TJP1 protein, human)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 0 (hemoglobin A1c protein, human)
SB  - IM
MH  - Biomarkers/*blood
MH  - Blood Glucose
MH  - Cholesterol, HDL/blood
MH  - Diabetes Mellitus, Type 2/*blood
MH  - Endotoxemia/blood
MH  - Female
MH  - Glycated Hemoglobin/metabolism
MH  - Humans
MH  - Inflammation/*blood
MH  - Insulin Resistance
MH  - Interleukin-6/blood
MH  - Lipopolysaccharides/*blood
MH  - Male
MH  - Middle Aged
MH  - Triglycerides/blood
MH  - Tumor Necrosis Factor-alpha/blood
MH  - Zonula Occludens-1 Protein/*blood
EDAT- 2013/12/19 06:00
MHDA- 2014/10/22 06:00
CRDT- 2013/12/19 06:00
PHST- 2013/09/18 00:00 [received]
PHST- 2013/11/15 00:00 [accepted]
PHST- 2013/12/19 06:00 [entrez]
PHST- 2013/12/19 06:00 [pubmed]
PHST- 2014/10/22 06:00 [medline]
AID - 10.1007/s11010-013-1911-4 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2014 Mar;388(1-2):203-10. doi: 10.1007/s11010-013-1911-4. Epub 
      2013 Dec 18.