PMID- 24347527
OWN - NLM
STAT- MEDLINE
DCOM- 20141028
LR  - 20220310
IS  - 1539-7262 (Electronic)
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 55
IP  - 3
DP  - 2014 Mar
TI  - Interleukin-4 regulates lipid metabolism by inhibiting adipogenesis and promoting 
      lipolysis.
PG  - 385-97
LID - 10.1194/jlr.M041392 [doi]
AB  - Long-term cytokine-mediated inflammation is a risk factor for obesity and type 2 
      diabetes mellitus (T2DM). Our previous studies reveal significant associations 
      between promoter single nucleotide polymorphisms (SNPs) of interleukin (IL)-4 and 
      T2DM, as well as between SNPs in genes encoding IL-4/IL-4 receptor and high 
      density lipoproteins. Our animal study reveals that IL-4 regulates glucose/lipid 
      metabolism by promoting glucose tolerance and inhibiting lipid deposits. The 
      above results strongly suggest the involvement of IL-4 in energy homeostasis. In 
      the present study, we focus on examining the regulatory mechanism of IL-4 to 
      lipid metabolism. Our results show that IL-4 inhibits adipogenesis by 
      downregulating the expression of peroxisome proliferator-activated receptor-gamma and 
      CCAAT/enhancer-binding protein-alpha. Additionally, IL-4 promotes lipolysis by 
      enhancing the activity and translocation of hormone sensitive lipase (HSL) in 
      mature adipocytes, which suggests that IL-4 plays a pro-lipolytic role in lipid 
      metabolism by boosting HSL activity. Our results demonstrate that IL-4 harbors 
      pro-lipolysis capacity by inhibiting adipocyte differentiation and lipid 
      accumulation as well as by promoting lipolysis in mature adipocytes to decrease 
      lipid deposits. The above findings uncover the novel roles of IL-4 in lipid 
      metabolism and provide new insights into the interactions among cytokine/immune 
      responses, insulin sensitivity, and metabolism.
FAU - Tsao, Chang-Hui
AU  - Tsao CH
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, 
      Taiwan.
FAU - Shiau, Ming-Yuh
AU  - Shiau MY
FAU - Chuang, Pei-Hua
AU  - Chuang PH
FAU - Chang, Yih-Hsin
AU  - Chang YH
FAU - Hwang, Jaulang
AU  - Hwang J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131217
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (CCAAT-Enhancer-Binding Protein-alpha)
RN  - 0 (Carrier Proteins)
RN  - 0 (PPAR gamma)
RN  - 0 (Perilipin-1)
RN  - 0 (Phosphoproteins)
RN  - 0 (STAT6 Transcription Factor)
RN  - 0 (Stat6 protein, mouse)
RN  - 207137-56-2 (Interleukin-4)
RN  - EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases)
RN  - EC 3.1.1.13 (Sterol Esterase)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipogenesis/*drug effects/genetics
MH  - Animals
MH  - Blotting, Western
MH  - CCAAT-Enhancer-Binding Protein-alpha/genetics/metabolism
MH  - Carrier Proteins/metabolism
MH  - Cyclic AMP-Dependent Protein Kinases/metabolism
MH  - Gene Expression/drug effects
MH  - Interleukin-4/*pharmacology
MH  - Lipid Metabolism/*drug effects/genetics
MH  - Lipolysis/*drug effects/genetics
MH  - Mice
MH  - Microscopy, Confocal
MH  - PPAR gamma/genetics/metabolism
MH  - Perilipin-1
MH  - Phosphoproteins/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Transport/drug effects
MH  - RNA Interference
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - STAT6 Transcription Factor/genetics/metabolism
MH  - Signal Transduction/drug effects/genetics
MH  - Sterol Esterase/metabolism
PMC - PMC3934724
OTO - NOTNLM
OT  - CCAAT/enhancer-binding protein-alpha
OT  - hormone sensitive lipase
OT  - perilipin
OT  - peroxisome proliferator-activated receptor-gamma
EDAT- 2013/12/19 06:00
MHDA- 2014/10/29 06:00
PMCR- 2014/03/01
CRDT- 2013/12/19 06:00
PHST- 2013/12/19 06:00 [entrez]
PHST- 2013/12/19 06:00 [pubmed]
PHST- 2014/10/29 06:00 [medline]
PHST- 2014/03/01 00:00 [pmc-release]
AID - S0022-2275(20)37673-2 [pii]
AID - m041392 [pii]
AID - 10.1194/jlr.M041392 [doi]
PST - ppublish
SO  - J Lipid Res. 2014 Mar;55(3):385-97. doi: 10.1194/jlr.M041392. Epub 2013 Dec 17.