PMID- 24349240 OWN - NLM STAT- MEDLINE DCOM- 20141012 LR - 20220310 IS - 1932-6203 (Electronic) IS - 1932-6203 (Linking) VI - 8 IP - 12 DP - 2013 TI - Overexpression of small heat shock protein LimHSP16.45 in Arabidopsis enhances tolerance to abiotic stresses. PG - e82264 LID - 10.1371/journal.pone.0082264 [doi] LID - e82264 AB - Small heat shock proteins (smHSPs) play important and extensive roles in plant defenses against abiotic stresses. We cloned a gene for a smHSP from the David Lily (Lilium davidii (E. H. Wilson) Raffill var. Willmottiae), which we named LimHSP16.45 based on its protein molecular weight. Its expression was induced by many kinds of abiotic stresses in both the lily and transgenic plants of Arabidopsis. Heterologous expression enhanced cell viability of the latter under high temperatures, high salt, and oxidative stress, and heat shock granules (HSGs) formed under heat or salinity treatment. Assays of enzymes showed that LimHSP16.45 overexpression was related to greater activity by superoxide dismutase and catalase in transgenic lines. Therefore, we conclude that heterologous expression can protect plants against abiotic stresses by preventing irreversible protein aggregation, and by scavenging cellular reactive oxygen species. FAU - Mu, Changjun AU - Mu C AD - Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China. FAU - Zhang, Shijia AU - Zhang S AD - Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China. FAU - Yu, Guanzhong AU - Yu G AD - Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China. FAU - Chen, Ni AU - Chen N AD - Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China. FAU - Li, Xiaofeng AU - Li X AD - Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China. FAU - Liu, Heng AU - Liu H AD - Institute of Cell Biology, School of Life Sciences, Lanzhou University, Lanzhou, P.R. China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20131213 PL - United States TA - PLoS One JT - PloS one JID - 101285081 RN - 0 (Heat-Shock Proteins) RN - 0 (Plant Proteins) RN - 147336-22-9 (Green Fluorescent Proteins) RN - EC 1.11.1.6 (Catalase) RN - EC 1.15.1.1 (Superoxide Dismutase) SB - IM MH - *Adaptation, Physiological/genetics MH - Arabidopsis/cytology/enzymology/genetics/*physiology MH - Catalase/metabolism MH - Cell Membrane/metabolism MH - Cell Survival/genetics MH - Cytoplasmic Granules/metabolism MH - Gene Expression Profiling MH - Gene Expression Regulation, Plant MH - Genetic Vectors MH - Green Fluorescent Proteins/metabolism MH - Heat-Shock Proteins/genetics/*metabolism MH - Hot Temperature MH - Lilium/*metabolism MH - Oxidative Stress/genetics MH - Plant Proteins/genetics/*metabolism MH - Plants, Genetically Modified MH - Salinity MH - *Stress, Physiological/genetics MH - Superoxide Dismutase/metabolism PMC - PMC3862632 COIS- Competing Interests: The authors have declared that no competing interests exist. EDAT- 2013/12/19 06:00 MHDA- 2014/10/13 06:00 PMCR- 2013/12/13 CRDT- 2013/12/19 06:00 PHST- 2013/08/30 00:00 [received] PHST- 2013/10/31 00:00 [accepted] PHST- 2013/12/19 06:00 [entrez] PHST- 2013/12/19 06:00 [pubmed] PHST- 2014/10/13 06:00 [medline] PHST- 2013/12/13 00:00 [pmc-release] AID - PONE-D-13-35908 [pii] AID - 10.1371/journal.pone.0082264 [doi] PST - epublish SO - PLoS One. 2013 Dec 13;8(12):e82264. doi: 10.1371/journal.pone.0082264. eCollection 2013.