PMID- 24349933
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131218
LR  - 20211021
IS  - 2187-137X (Print)
IS  - 2186-5116 (Electronic)
IS  - 2186-5116 (Linking)
VI  - 2
IP  - Spec Iss
DP  - 2013
TI  - Image and Spectral Processing for ToF-SIMS Analysis of Biological Materials.
PG  - S0014
LID - 10.5702/massspectrometry.S0014 [doi]
LID - S0014
AB  - Time-of-flight secondary ion mass spectrometry (ToF-SIMS) instruments can rapidly 
      produce large complex data sets. Within each spectrum, there can be hundreds of 
      peaks. A typical 256x256 pixel image contains 65,536 spectra. If this is extended 
      to a 3D image, the number of spectra in a given data set can reach the millions. 
      The challenge becomes how to process these large data sets while taking into 
      account the changes and differences between all the peaks in the spectra. This is 
      particularly challenging for biological materials that all contain the same types 
      of proteins and lipids, just in varying concentrations and spatial distributions. 
      This data analysis challenge is further complicated by the limitations in the ion 
      yield of higher mass, more chemically specific species, and potentially by the 
      processing power of typical computers. Herein we briefly discuss analysis 
      methodologies including univariate analysis, multivariate analysis (MVA) methods, 
      and some of the limitations of ToF-SIMS analysis of biological materials.
FAU - Graham, Daniel J
AU  - Graham DJ
AD  - National ESCA and Surface Analysis Center for Biomedical Problems.
FAU - Castner, David G
AU  - Castner DG
AD  - National ESCA and Surface Analysis Center for Biomedical Problems ; Chemical 
      Engineering University of Washington.
LA  - eng
GR  - P41 EB002027/EB/NIBIB NIH HHS/United States
PT  - Journal Article
DEP - 20130415
PL  - Japan
TA  - Mass Spectrom (Tokyo)
JT  - Mass spectrometry (Tokyo, Japan)
JID - 101603453
PMC - PMC3809723
OTO - NOTNLM
OT  - ToF-SIMS
OT  - biological materials
OT  - image processing
EDAT- 2013/12/19 06:00
MHDA- 2013/12/19 06:01
PMCR- 2013/04/15
CRDT- 2013/12/19 06:00
PHST- 2012/09/27 00:00 [received]
PHST- 2012/10/23 00:00 [accepted]
PHST- 2013/12/19 06:00 [entrez]
PHST- 2013/12/19 06:00 [pubmed]
PHST- 2013/12/19 06:01 [medline]
PHST- 2013/04/15 00:00 [pmc-release]
AID - 10.5702/massspectrometry.S0014 [doi]
PST - ppublish
SO  - Mass Spectrom (Tokyo). 2013;2(Spec Iss):S0014. doi: 
      10.5702/massspectrometry.S0014. Epub 2013 Apr 15.