PMID- 24350892
OWN - NLM
STAT- MEDLINE
DCOM- 20140919
LR  - 20160511
IS  - 2152-4998 (Electronic)
IS  - 2152-4971 (Linking)
VI  - 16
IP  - 1
DP  - 2014 Feb
TI  - Mitochondrial biogenesis and energy production in differentiating murine stem 
      cells: a functional metabolic study.
PG  - 84-90
LID - 10.1089/cell.2013.0049 [doi]
AB  - The significance of metabolic networks in guiding the fate of the stem cell 
      differentiation is only beginning to emerge. Oxidative metabolism has been 
      suggested to play a major role during this process. Therefore, it is critical to 
      understand the underlying mechanisms of metabolic alterations occurring in stem 
      cells to manipulate the ultimate outcome of these pluripotent cells. Here, using 
      P19 murine embryonal carcinoma cells as a model system, the role of mitochondrial 
      biogenesis and the modulation of metabolic networks during dimethyl sulfoxide 
      (DMSO)-induced differentiation are revealed. Blue native polyacrylamide gel 
      electrophoresis (BN-PAGE) technology aided in profiling key enzymes, such as 
      hexokinase (HK) [EC 2.7.1.1], glucose-6-phosphate isomerase (GPI) [EC 5.3.1.9], 
      pyruvate kinase (PK) [EC 2.7.1.40], Complex I [EC 1.6.5.3], and Complex IV [EC 
      1.9.3.1], that are involved in the energy budget of the differentiated cells. 
      Mitochondrial adenosine triphosphate (ATP) production was shown to be increased 
      in DMSO-treated cells upon exposure to the tricarboxylic acid (TCA) cycle 
      substrates, such as succinate and malate. The increased mitochondrial activity 
      and biogenesis were further confirmed by immunofluorescence microscopy. 
      Collectively, the results indicate that oxidative energy metabolism and 
      mitochondrial biogenesis were sharply upregulated in DMSO-differentiated P19 
      cells. This functional metabolic and proteomic study provides further evidence 
      that modulation of mitochondrial energy metabolism is a pivotal component of the 
      cellular differentiation process and may dictate the final destiny of stem cells.
FAU - Han, Sungwon
AU  - Han S
AD  - 1 Department of Chemistry and Biochemistry, Laurentian University , Sudbury, 
      Ontario P3E 2C6, Canada .
FAU - Auger, Christopher
AU  - Auger C
FAU - Thomas, Sean C
AU  - Thomas SC
FAU - Beites, Crestina L
AU  - Beites CL
FAU - Appanna, Vasu D
AU  - Appanna VD
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131219
PL  - United States
TA  - Cell Reprogram
JT  - Cellular reprogramming
JID - 101528176
RN  - 0 (Cryoprotective Agents)
RN  - 0 (Mitochondrial Proteins)
RN  - YOW8V9698H (Dimethyl Sulfoxide)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*drug effects
MH  - Cell Line
MH  - Cryoprotective Agents/*pharmacology
MH  - Dimethyl Sulfoxide/*pharmacology
MH  - Mice
MH  - Mitochondria/*metabolism
MH  - Mitochondrial Dynamics/drug effects
MH  - Mitochondrial Proteins/*biosynthesis
MH  - Oxidative Phosphorylation/*drug effects
MH  - Stem Cells
EDAT- 2013/12/20 06:00
MHDA- 2014/09/23 06:00
CRDT- 2013/12/20 06:00
PHST- 2013/12/20 06:00 [entrez]
PHST- 2013/12/20 06:00 [pubmed]
PHST- 2014/09/23 06:00 [medline]
AID - 10.1089/cell.2013.0049 [doi]
PST - ppublish
SO  - Cell Reprogram. 2014 Feb;16(1):84-90. doi: 10.1089/cell.2013.0049. Epub 2013 Dec 
      19.