PMID- 2435115
OWN - NLM
STAT- MEDLINE
DCOM- 19870403
LR  - 20190824
IS  - 0065-4299 (Print)
IS  - 0065-4299 (Linking)
VI  - 19
IP  - 3-4
DP  - 1986 Nov
TI  - Renal histamine: release by immune stimuli.
PG  - 141-9
AB  - In vitro perfused kidneys of ovalbumin-sensitized guinea-pigs consistently 
      released relatively large quantities of histamine when challenged with the 
      specific antigen (mean +/- SEM in twelve experiments was 37.7 +/- 6.0% of total 
      kidney histamine, maximum 70.6%, compared with a basal release of 0.5 +/- 0.46% 
      over a comparable period) but not with non-cross-reacting antigens. There was 
      also no release from non-sensitized kidney. Rabbit antisera to guinea pig IgG1 
      and IgG2 immunoglobulins (but not normal rabbit serum) also consistently released 
      histamine from perfused kidneys of sensitized guinea-pigs, but the release was 
      smaller than with antigen, and could also be obtained from kidneys of 
      non-sensitized guinea-pigs (maximum release 62.4% with the most potent 
      antiserum). Guinea-pig kidney cell suspensions prepared by collagenase dispersion 
      in vitro responded similarly, but the release with antigen was small (less than 
      10% net release, minus the spontaneous release 9.46% on average) as compared to 
      anti-IgG1 (net release up to 38%) or anti-IgG2 (up to 44%). Rat kidney cells 
      prepared by a similar procedure, and passively sensitized in vitro by incubation 
      with rat immunoglobulin E (IgE) myeloma protein also responded to the addition of 
      antiserum to rat IgE by releasing substantial amounts of histamine (up to 44% net 
      release). In addition, heparin-containing cells (presumably mast cells or 
      equivalent) in the enzyme-dispersed kidney cell preparations in both species were 
      identified and counted by an adaptation of the Technicon H 6000 system used for 
      counting blood basophils, and shown to represent 1 in 10,000 or less of the total 
      cell population, which was not different from the count of similar cells in lung 
      and heart tissues.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Assem, E S
AU  - Assem ES
FAU - Abdullah, N A
AU  - Abdullah NA
FAU - Ghanem, N S
AU  - Ghanem NS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Agents Actions
JT  - Agents and actions
JID - 0213341
RN  - 0 (Immunoglobulin G)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Animals
MH  - Guinea Pigs
MH  - *Histamine Release/drug effects
MH  - Immunoglobulin G/immunology
MH  - In Vitro Techniques
MH  - Kidney/immunology/*metabolism
MH  - Mast Cells/drug effects/metabolism
MH  - Ovalbumin/immunology
MH  - Perfusion
EDAT- 1986/11/01 00:00
MHDA- 1986/11/01 00:01
CRDT- 1986/11/01 00:00
PHST- 1986/11/01 00:00 [pubmed]
PHST- 1986/11/01 00:01 [medline]
PHST- 1986/11/01 00:00 [entrez]
AID - 10.1007/BF01966198 [doi]
PST - ppublish
SO  - Agents Actions. 1986 Nov;19(3-4):141-9. doi: 10.1007/BF01966198.