PMID- 24354001
OWN - NLM
STAT- MEDLINE
DCOM- 20140505
LR  - 20191210
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1318
DP  - 2013 Nov 29
TI  - Stir bar sorptive extraction combined with high performance liquid 
      chromatography-ultraviolet/inductively coupled plasma mass spectrometry for 
      analysis of thyroxine in urine samples.
PG  - 49-57
AB  - tIn this work, polyethyleneglycol (PEG)/hydroxyl polydimethylsiloxane (OH-PDMS)/gamma 
      -mercaptopropyltrimethoxysilane (gamma -MPTS) coated stir bar was prepared by sol-gel 
      process and its extraction performance for the extraction of amphoteric 
      thyroxines (3,3',5,5'-tetraiodothyronin, T(4); 3,3',5-triiodothyronine, T(3); 
      reversed-3,3',5-triiodothyronine, rT(3)) and their metabolite 
      (3,5-diiodothyronine,T2) was studied. The preparation reproducibility of 
      PEG/OH-PDMS/gamma -MPTS coated stir bar was investigated, and the relative standard 
      deviations (RSDs) in the same batch and among different batches were 3.3-14.3% (n 
      = 5) and 7.7-16.6% (n = 3), respectively. The prepared PEG/OH-PDMS/gamma -MPTS coated 
      stir bar could be reused for more than 20 times. Based on this fact, a novel 
      method of stir bar sorptive extraction (SBSE) combined with high performance 
      liquid chromatography (HPLC)-ultraviolet (UV)and HPLC-inductively coupled plasma 
      mass spectrometry (ICP-MS) for the analysis of target thyroxinesin human urine 
      samples was developed. The influencing factors of SBSE, such as sample pH, 
      extraction time, stirring rate, salt effect, desorption solution and desorption 
      time, were studied in detail, and the analytical performance of the proposed 
      method was evaluated under the optimized conditions. The enrichment factors (EFs) 
      of the developed method for four target thyroxines were in the range of 
      14.9-70.4(theoretical enrichment factor was 100). The RSDs were ranging from 4.0% 
      to 13.8% for SBSE-HPLC-UV (c = 25 mug/L, n = 6) and from 3.7% to 6.1% for 
      SBSE-HPLC-ICP-MS (c = 0.5 mug/L, n = 5). The linear range obtained by SBSE-HPLC-UV 
      was 2-500 mug/L for T(2)and 5-500 mug/L for rT3, T(3)and T(4), with correlation 
      coefficients (r) ranging from 0.9957 to 0.9998, respectively, while the linear 
      range obtained by SBSE-HPLC-ICP-MS was 0.05-500 mug/L for T(2) and rT(3), 0.10-200 
      mug/L for T(3) and 0.05-200 mug/L for T(4)with r ranging from 0.9979 to 0.9998, 
      respectively. The limits of detection (LODs) for the target thyroxines were 
      0.60-2.20 mug/L for SBSE-HPLC-UV and 0.0071-0.0355 mug/L SBSE-HPLC-ICP-MS, 
      respectively. The developed method was applied for the determination of target 
      thyroxines in urine samples, and the recovery for the spiking samples obtained by 
      SBSE-HPLC-UV was in the range of 81.6-137.6% for human urine,while the recovery 
      for the spiking urine samples obtained by SBSE-HPLC-ICP-MS were in the range of 
      72.0-121.5%.
FAU - Fan, Wenying
AU  - Fan W
FAU - Mao, Xiangju
AU  - Mao X
FAU - He, Man
AU  - He M
FAU - Chen, Beibei
AU  - Chen B
FAU - Hu, Bin
AU  - Hu B
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - Q51BO43MG4 (Thyroxine)
SB  - IM
MH  - Adsorption
MH  - Adult
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Female
MH  - Humans
MH  - Limit of Detection
MH  - Male
MH  - Mass Spectrometry/*methods
MH  - Molecular Structure
MH  - Solid Phase Extraction/instrumentation/*methods
MH  - Spectrophotometry, Ultraviolet
MH  - Thyroxine/chemistry/*isolation & purification/*urine
EDAT- 2013/12/20 06:00
MHDA- 2014/05/06 06:00
CRDT- 2013/12/20 06:00
PHST- 2013/12/20 06:00 [entrez]
PHST- 2013/12/20 06:00 [pubmed]
PHST- 2014/05/06 06:00 [medline]
AID - S0021-9673(13)01622-1 [pii]
AID - 10.1016/j.chroma.2013.10.022 [doi]
PST - ppublish
SO  - J Chromatogr A. 2013 Nov 29;1318:49-57. doi: 10.1016/j.chroma.2013.10.022.