PMID- 24354925
OWN - NLM
STAT- MEDLINE
DCOM- 20150416
LR  - 20211021
IS  - 1600-0668 (Electronic)
IS  - 0905-6947 (Print)
IS  - 0905-6947 (Linking)
VI  - 24
IP  - 4
DP  - 2014 Aug
TI  - Domestic exposure to fungal allergenic particles determined by halogen 
      immunoassay using subject's serum versus particles carrying three non-fungal 
      allergens determined by allergen-specific HIA.
PG  - 438-45
LID - 10.1111/ina.12087 [doi]
AB  - Studies that estimate indoor aeroallergen exposure typically measure a 
      pre-selected limited range of allergens. In this study, inhalable aeroallergen 
      particles were quantified using the halogen immunoassay (HIA) to determine the 
      contribution of fungal and non-fungal aeroallergens to total allergen exposure. 
      Bioaerosols from 39 homes of fungal-allergic subjects were sampled using 
      inhalable fraction samplers and immunostained by HIA using resident subject's 
      immunoglobulin E (IgE) to detect allergen-laden particles. Fungal aerosols as 
      well as particles carrying mite, cat, and cockroach allergens were identified and 
      enumerated by HIA. Reservoir dust-mite (Der p 1), cat (Fel d 1), and cockroach 
      (Bla g 1) allergen concentrations were quantified by ELISA. Fungal particles that 
      bound subject's IgE in the HIA were 1.7 (bedroom)- and 1.4 (living room)-fold 
      more concentrated than Der p 1, Fel d 1, and Bla g 1 allergen particles combined. 
      Predominant fungal conidia that bound IgE were derived from common environmental 
      genera including Cladosporium and other fungi that produce amerospores. Airborne 
      mite, cat, and cockroach allergen particle counts were not associated with 
      reservoir concentrations determined by ELISA. This study demonstrates that 
      inhalable fungal aerosols are the predominant aeroallergen sources in Sydney 
      homes and should be considered in future exposure assessments. PRACTICAL 
      IMPLICATIONS: Indoor allergen exposure assessment studies have primarily focused 
      on a limited range of allergen sources in samples derived from reservoir dust 
      samples. Using an innovative immunodiagnostic approach, this study demonstrates 
      that fungal bioaerosols are the dominant source of aeroallergen exposure in the 
      domestic environment, providing unique insight into domestic aeroallergen 
      exposure.
CI  - (c) 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Sercombe, J K
AU  - Sercombe JK
AD  - Woolcock Institute of Medical Research, The University of Sydney, Sydney, 
      Australia; Department of Infectious Diseases and Immunology, The University of 
      Sydney, Sydney, Australia.
FAU - Liu-Brennan, D
AU  - Liu-Brennan D
FAU - McKay, K O
AU  - McKay KO
FAU - Green, B J
AU  - Green BJ
FAU - Tovey, E R
AU  - Tovey ER
LA  - eng
GR  - CC999999/Intramural CDC HHS/United States
PT  - Journal Article
DEP - 20140113
PL  - England
TA  - Indoor Air
JT  - Indoor air
JID - 9423515
RN  - 0 (Allergens)
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Arthropod Proteins)
RN  - 0 (Glycoproteins)
RN  - 0 (allergen Bla g 1)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - EC 3.4.22.- (Cysteine Endopeptidases)
RN  - EC 3.4.22.- (Dermatophagoides pteronyssinus antigen p 1)
RN  - G408EE88II (Fel d 1 protein, Felis domesticus)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - *Air Microbiology
MH  - Air Pollution, Indoor/*analysis
MH  - Allergens
MH  - Animals
MH  - Antigens, Dermatophagoides
MH  - Arthropod Proteins
MH  - Child
MH  - Cysteine Endopeptidases
MH  - Female
MH  - Fungi/*immunology
MH  - Glycoproteins
MH  - Humans
MH  - Immunoassay
MH  - Immunoglobulin E/*blood/immunology
MH  - Male
MH  - Middle Aged
MH  - Queensland
MH  - Young Adult
PMC - PMC4678322
MID - NIHMS739511
OTO - NOTNLM
OT  - Aeroallergens
OT  - Airborne fungi
OT  - Allergen exposure
OT  - Bioaerosols
OT  - Fungal allergens
EDAT- 2013/12/21 06:00
MHDA- 2015/04/17 06:00
PMCR- 2015/12/15
CRDT- 2013/12/21 06:00
PHST- 2013/06/17 00:00 [received]
PHST- 2013/12/13 00:00 [accepted]
PHST- 2013/12/21 06:00 [entrez]
PHST- 2013/12/21 06:00 [pubmed]
PHST- 2015/04/17 06:00 [medline]
PHST- 2015/12/15 00:00 [pmc-release]
AID - 10.1111/ina.12087 [doi]
PST - ppublish
SO  - Indoor Air. 2014 Aug;24(4):438-45. doi: 10.1111/ina.12087. Epub 2014 Jan 13.