PMID- 24355003
OWN - NLM
STAT- MEDLINE
DCOM- 20140829
LR  - 20131220
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 83
IP  - 1
DP  - 2014 Jan
TI  - HLA-DRB1, -DRB3, -DRB4 and -DRB5 genotyping at a super-high resolution level by 
      long range PCR and high-throughput sequencing.
PG  - 10-6
LID - 10.1111/tan.12258 [doi]
AB  - Super high-resolution single molecule sequence-based typing (SS-SBT) is a human 
      leukocyte antigen (HLA) DNA typing method to the field 4 level of allelic 
      resolution (formerly known as eight-digit typing) to efficiently detect new and 
      null alleles without phase ambiguity by combination of long ranged polymerase 
      chain reaction (PCR) amplification and next-generation sequencing (NGS) 
      technologies. We previously reported the development and application of the 
      SS-SBT method for the eight classical HLA loci, A, B, C, DRB1, DQA1, DQB1, DPA1 
      and DPB1. In this article, we describe the development of the SS-SBT method for 
      three DRB1 linked loci, DRB3, DRB4 and DRB5 (DRB3/4/5) and characterization of 
      DRB1-DRB3/4/5 haplotype structures to the field 4 level. Locus specific PCR 
      primers for DRB3/4/5 were designed to amplify the gene regions from intron 1 to 
      exon 6 [3' untranslated region (3'UTR)]. In total 20 DRB1 and 13 DRB3/4/5 allele 
      sequences were determined by the SS-SBT to the field 4 level without phase 
      ambiguity using 19 DR51, DR52 and DR53 positive genomic DNA samples obtained from 
      Japanese. Moreover, 18 DRB1-DRB3/4/5 haplotypes were estimated to the field 4 
      level by the SS-SBT method in contrast to 10 haplotypes estimated by conventional 
      methods to the field 1 level (formerly known as two digit typing). Therefore, 
      DRB1-DRB3/4/5 haplotyping by SS-SBT is expected to provide informative data for 
      improved HLA matching in medical research, transplantation procedures, 
      HLA-related disease studies and human population diversity studies.
CI  - (c) 2013 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Ozaki, Y
AU  - Ozaki Y
AD  - Department of Molecular Life Science, Division of Basic Medical Science and 
      Molecular Medicine, Tokai University School of Medicine, Isehara, Japan.
FAU - Suzuki, S
AU  - Suzuki S
FAU - Shigenari, A
AU  - Shigenari A
FAU - Okudaira, Y
AU  - Okudaira Y
FAU - Kikkawa, E
AU  - Kikkawa E
FAU - Oka, A
AU  - Oka A
FAU - Ota, M
AU  - Ota M
FAU - Mitsunaga, S
AU  - Mitsunaga S
FAU - Kulski, J K
AU  - Kulski JK
FAU - Inoko, H
AU  - Inoko H
FAU - Shiina, T
AU  - Shiina T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131130
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (DNA Primers)
RN  - 0 (HLA-DRB1 Chains)
RN  - 0 (HLA-DRB3 Chains)
RN  - 0 (HLA-DRB4 Chains)
RN  - 0 (HLA-DRB5 Chains)
SB  - IM
MH  - Alleles
MH  - DNA Primers/genetics
MH  - Genotype
MH  - HLA-DRB1 Chains/*genetics
MH  - HLA-DRB3 Chains/*genetics
MH  - HLA-DRB4 Chains/*genetics
MH  - HLA-DRB5 Chains/*genetics
MH  - High-Throughput Nucleotide Sequencing
MH  - Histocompatibility Testing/*methods/trends
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Transplantation Immunology
OTO - NOTNLM
OT  - DNA typing
OT  - human leukocyte antigen
OT  - next-generation sequencing
OT  - polymerase chain reaction
OT  - super high resolution single molecule - sequence-based typing
EDAT- 2013/12/21 06:00
MHDA- 2014/08/30 06:00
CRDT- 2013/12/21 06:00
PHST- 2013/07/10 00:00 [received]
PHST- 2013/08/30 00:00 [accepted]
PHST- 2013/12/21 06:00 [entrez]
PHST- 2013/12/21 06:00 [pubmed]
PHST- 2014/08/30 06:00 [medline]
AID - 10.1111/tan.12258 [doi]
PST - ppublish
SO  - Tissue Antigens. 2014 Jan;83(1):10-6. doi: 10.1111/tan.12258. Epub 2013 Nov 30.