PMID- 24357600
OWN - NLM
STAT- MEDLINE
DCOM- 20141013
LR  - 20211021
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 164
IP  - 2
DP  - 2014 Feb
TI  - TAP46 plays a positive role in the ABSCISIC ACID INSENSITIVE5-regulated gene 
      expression in Arabidopsis.
PG  - 721-34
LID - 10.1104/pp.113.233684 [doi]
AB  - TAP46 is a protein phosphatase2A (PP2A)-associated protein that regulates PP2A 
      activity in Arabidopsis (Arabidopsis thaliana). To study how PP2A is involved in 
      abscisic acid (ABA) signaling in plants, we studied the function of TAP46 in 
      ABA-regulated seed maturation and seedling development. Expression of TAP46 
      coincides with the action of ABA in developing seeds and during seed germination, 
      and the TAP46 transcript reaches to the highest level in mature seeds. Real-time 
      polymerase chain reaction analysis indicates that external ABA can increase TAP46 
      transcript level transiently during seed germination. Overexpression of TAP46 
      increases plant sensitivity to ABA, while tap46 knockdown mutants are less 
      sensitive to ABA during seed germination, suggesting that TAP46 functions 
      positively in ABA signaling. Overexpression of TAP46 also leads to lower PP2A 
      activity, while tap46-1 knockdown mutant displays higher PP2A activity, 
      suggesting that TAP46 negatively regulates PP2A activity in Arabidopsis. Both 
      TAP46 and PP2A interact with the ABA-regulated transcription factor ABA 
      INSENSITIVE5 (ABI5) in vivo, and TAP46's binding to ABI5 can stabilize ABI5. 
      Furthermore, TAP46's binding to the phosphorylated ABI5 may prevent PP2A or 
      PP2A-like protein phosphatases from removing the phosphate from ABI5, thereby 
      maintaining ABI5 in its active form. Overexpression of TAP46 and inhibition of 
      activities of PP2A or PP2A-like protein phosphatases can increase transcript 
      levels of several ABI5-regulated genes, suggesting that TAP46 is a positive 
      factor in the ABA-regulated gene expression in Arabidopsis.
FAU - Hu, Rongbin
AU  - Hu R
AD  - Department of Biological Sciences, Texas Tech University, Lubbock, Texas 79409.
FAU - Zhu, Yinfeng
AU  - Zhu Y
FAU - Shen, Guoxin
AU  - Shen G
FAU - Zhang, Hong
AU  - Zhang H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131219
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (ABI5 protein, Arabidopsis)
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Basic-Leucine Zipper Transcription Factors)
RN  - 0 (TAP46 protein, Arabidopsis)
RN  - 72S9A8J5GW (Abscisic Acid)
RN  - EC 3.1.3.16 (Protein Phosphatase 2)
SB  - IM
MH  - Abscisic Acid
MH  - Arabidopsis/drug effects/enzymology/*genetics
MH  - Arabidopsis Proteins/genetics/*metabolism
MH  - Basic-Leucine Zipper Transcription Factors/genetics/*metabolism
MH  - *Gene Expression Regulation, Plant
MH  - Genes, Plant/genetics
MH  - Germination/genetics
MH  - Models, Biological
MH  - Phosphorylation/drug effects
MH  - Plants, Genetically Modified
MH  - Protein Binding/drug effects
MH  - Protein Phosphatase 2/metabolism
MH  - Protein Stability/drug effects
MH  - Real-Time Polymerase Chain Reaction
MH  - Seeds/genetics/growth & development
PMC - PMC3912101
EDAT- 2013/12/21 06:00
MHDA- 2014/10/14 06:00
PMCR- 2013/12/19
CRDT- 2013/12/21 06:00
PHST- 2013/12/21 06:00 [entrez]
PHST- 2013/12/21 06:00 [pubmed]
PHST- 2014/10/14 06:00 [medline]
PHST- 2013/12/19 00:00 [pmc-release]
AID - pp.113.233684 [pii]
AID - 233684 [pii]
AID - 10.1104/pp.113.233684 [doi]
PST - ppublish
SO  - Plant Physiol. 2014 Feb;164(2):721-34. doi: 10.1104/pp.113.233684. Epub 2013 Dec 
      19.