PMID- 24359723
OWN - NLM
STAT- MEDLINE
DCOM- 20140925
LR  - 20190318
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1325
DP  - 2014 Jan 17
TI  - Determination of three nitroimidazoles in rabbit plasma by two-step stacking in 
      capillary zone electrophoresis featuring sweeping and micelle to solvent 
      stacking.
PG  - 227-33
LID - S0021-9673(13)01858-X [pii]
LID - 10.1016/j.chroma.2013.11.053 [doi]
AB  - This study reports a sweeping and micelle to solvent stacking (MSS) method for 
      fast stacking of nitroimidazoles in capillary zone electrophoresis (CZE). The 
      optimal experimental conditions are run buffer of 25mM sodium phosphate (pH 1.5) 
      and 0.50% (v/v) methanol, micellar solution of 30mM sodium dodecyl sulfate, 
      applied voltage of 28kV, and sample and micellar solution injection of 100s. By 
      applying this new on-line preconcentration technique, the nitroimidazoles content 
      can be determined within 9min with the limit of detection (S/N=3) ranging 
      2.3-3.0ng/mL, which is lower than that of conventional CZE analysis. The proposed 
      MSS technique affords 20-, 12- and 38-fold improvements in sensitivity for the 
      detection of dimetridazole, metronidazole and secnidazole, respectively. The 
      relative standard deviations (RSDs) of intra-day and inter-day are 2.1-3.6% and 
      2.7-4.6% (n=6), respectively. The recoveries in pretreated rabbit plasma at 
      spiked levels of 5.0-10.0mug/mL are 92.0-101.1% with RSDs lower than 3.4%. The 
      proposed sweeping and MSS-CZE is a highly sensitive method for the detection of 
      nitroimidazoles in biological and clinical samples and has been successfully 
      applied to analyze nitroimidazoles in pretreated rabbit plasma.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Yang, Xiupei
AU  - Yang X
AD  - College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution 
      Control Key Laboratory of Sichuan Province, China West Normal University, 
      Nanchong 637000, China. Electronic address: xiupeiyang@163.com.
FAU - Cheng, Xiumei
AU  - Cheng X
AD  - College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution 
      Control Key Laboratory of Sichuan Province, China West Normal University, 
      Nanchong 637000, China.
FAU - Lin, Yingyun
AU  - Lin Y
AD  - College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution 
      Control Key Laboratory of Sichuan Province, China West Normal University, 
      Nanchong 637000, China.
FAU - Tan, Zhijing
AU  - Tan Z
AD  - College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution 
      Control Key Laboratory of Sichuan Province, China West Normal University, 
      Nanchong 637000, China.
FAU - Xie, Linxiang
AU  - Xie L
AD  - College of Chemistry and Chemical Engineering, Chemical Synthesis and Pollution 
      Control Key Laboratory of Sichuan Province, China West Normal University, 
      Nanchong 637000, China.
FAU - Choi, Martin M F
AU  - Choi MM
AD  - Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon 
      Tong, Hong Kong Special Administrative Region. Electronic address: 
      mfchoi@hkbu.edu.hk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131203
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Buffers)
RN  - 0 (Micelles)
RN  - 0 (Nitroimidazoles)
RN  - 0 (Solvents)
RN  - 368GB5141J (Sodium Dodecyl Sulfate)
RN  - Y4S76JWI15 (Methanol)
SB  - IM
MH  - Animals
MH  - Buffers
MH  - Electrophoresis, Capillary/*methods
MH  - Hydrogen-Ion Concentration
MH  - Methanol/chemistry
MH  - Micelles
MH  - Nitroimidazoles/*blood/chemistry
MH  - Rabbits
MH  - Sodium Dodecyl Sulfate/chemistry
MH  - Solvents/chemistry
OTO - NOTNLM
OT  - Capillary electrophoresis
OT  - Micelle to solvent stacking
OT  - Nitroimidazole
OT  - Rabbit plasma
OT  - Sweeping
EDAT- 2013/12/24 06:00
MHDA- 2014/09/26 06:00
CRDT- 2013/12/24 06:00
PHST- 2013/10/31 00:00 [received]
PHST- 2013/11/26 00:00 [revised]
PHST- 2013/11/27 00:00 [accepted]
PHST- 2013/12/24 06:00 [entrez]
PHST- 2013/12/24 06:00 [pubmed]
PHST- 2014/09/26 06:00 [medline]
AID - S0021-9673(13)01858-X [pii]
AID - 10.1016/j.chroma.2013.11.053 [doi]
PST - ppublish
SO  - J Chromatogr A. 2014 Jan 17;1325:227-33. doi: 10.1016/j.chroma.2013.11.053. Epub 
      2013 Dec 3.