PMID- 24360114
OWN - NLM
STAT- MEDLINE
DCOM- 20140721
LR  - 20141120
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 93
IP  - 34
DP  - 2013 Sep 10
TI  - [Functional role of caveolin-1 in airway smooth muscle cells proliferation and 
      regulatory effect of roxithromycin].
PG  - 2750-4
AB  - OBJECTIVE: To explore the functional role of caveolin-1 in airway smooth muscle 
      cells (ASMCs) proliferation and examine the regulatory effect of roxithromycin. 
      METHODS: The rat model of bronchial asthma was established. Electron microscope 
      was employed to observe the status of caveolae and light microscope for the 
      histological changes in pulmonary tissues. The primarily cultured ASMCs were 
      divided into 5 groups: control (group A), asthmatic ASMCs (group B), PD98059 
      (group C), roxithromycin (group D) and methyl-beta-cyclodextrin (group E). Cell 
      proliferation was detected by Cell Counting Kit-8 (CCK-8). And the expressions of 
      caveolin-1, extracellular regulated protein kinases (ERK) and monocyte 
      chemotactic protein (MCP)-1 were detected by Western blot and reverse 
      transcription polymerase chain reaction (RT-PCR). RESULTS: The cell proliferation 
      of asthmatic ASMCs (0.68 +/- 0.15, 0.63 +/- 0.13) in groups C and D were 
      significantly less than those in group B (0.96 +/- 0.14) (both P < 0.05) while 
      group E was more than group B (1.26 +/- 0.11 vs 0.96 +/- 0.14, P < 0.05). The content 
      of caveolin-1 (0.392 +/- 0.064, 0.332 +/- 0.057) in groups C and D were higher than 
      those in group B (0.237 +/- 0.032) (both P < 0.05) while ERK1/2 protein level in 
      groups C and D (0.241 +/- 0.017, 0.268 +/- 0.007) were less than those in group B 
      (0.346 +/- 0.009) (both P < 0.01). And MCP-1 protein level in groups C and D (0.198 
      +/- 0.015, 0.286 +/- 0.019) were less than those in group B (0.482 +/- 0.026) (both P < 
      0.01). The ERK mRNA level in groups C and D (0.277 +/- 0.043, 0.338 +/- 0.026) were 
      less than those in group B (0.591 +/- 0.022) (both P < 0.01). And also MCP-1 mRNA 
      in groups C and D (0.212 +/- 0.042, 0.249 +/- 0.032) were less than those in group B 
      (0.676 +/- 0.053) (all P < 0.01) CONCLUSIONS: Caveolin-1 preventing the 
      proliferation of asthmatic ASMCs is most likely mediated by ERK1/2 signal pathway 
      and a down-regulation of MCP-1 expression. And roxithromycin reduces the 
      proliferation of asthmatic ASMCs through up-regulating the expression of 
      caveolin-1 and inhibiting the expression of MCP-1.
FAU - Zeng, Wei-xian
AU  - Zeng WX
AD  - Department of Emergency Medicine, Second Affiliated Hospital, Wenzhou Medical 
      College, Wenzhou 325027, China.
FAU - Dai, Yuan-rong
AU  - Dai YR
AD  - Department of Emergency Medicine, Second Affiliated Hospital, Wenzhou Medical 
      College, Wenzhou 325027, China. Email: daiyr@126.com.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Cav1 protein, rat)
RN  - 0 (Caveolin 1)
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 21KOF230FA (Roxithromycin)
RN  - EC 2.7.11.24 (Mitogen-Activated Protein Kinase 3)
SB  - IM
MH  - Animals
MH  - Asthma/metabolism/*pathology
MH  - Caveolin 1/*metabolism
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Male
MH  - Mitogen-Activated Protein Kinase 3/metabolism
MH  - Myocytes, Smooth Muscle/metabolism/*pathology
MH  - Rats
MH  - Rats, Wistar
MH  - Respiratory System/metabolism/*pathology
MH  - Roxithromycin/*pharmacology
EDAT- 2013/12/24 06:00
MHDA- 2014/07/22 06:00
CRDT- 2013/12/24 06:00
PHST- 2013/12/24 06:00 [entrez]
PHST- 2013/12/24 06:00 [pubmed]
PHST- 2014/07/22 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2013 Sep 10;93(34):2750-4.