PMID- 24360185
OWN - NLM
STAT- MEDLINE
DCOM- 20140721
LR  - 20161203
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 93
IP  - 35
DP  - 2013 Sep 17
TI  - [Effects of batroxobin and electric cauterization on vascular remodeling of 
      rabbit with a removal of carotid arterial adventitia].
PG  - 2834-8
AB  - OBJECTIVE: To explore the topical hemostatic effects of batroxobin (BX) and 
      electric cauterization (EC) on capillary hemorrhage of rabbit with a removal of 
      carotid arterial adventitia. METHODS: A total of 27 New Zealand rabbits were 
      randomly divided into control, BX and EC groups. Each group received BX (2 kU/L), 
      EC (power = 40 W) and saline for topical hemostasis after a removal of carotid 
      arterial adventitia and blunt dissection. The animals were euthanized by 0, 14 
      and 28 d post-operation. The specimens of adventitia removal section were divided 
      into three parts for histology (hematoxylin and eosin, MASSON & transmission 
      electron microscope), immunohistochemistry (IHC) [monocyte chemoattractant 
      protein-1 (MCP-1), transforming growth factor-beta1 (TGF-beta1) and vascular 
      endothelial growth factor (VEGF)] and reverse transcription-polymerase chain 
      reaction (RT-PCR). RESULTS: At Day 28 post-operation, the remodeling index, 
      stenotic rate and collagen fiber density of BX and EC groups were (0.753 +/- 
      0.0739) and (0.618 +/- 0.0989), (0.298 +/- 0.030)% and (0.363 +/- 0.039)%, (15.4 +/- 
      3.5)% and (23.4 +/- 5.1)% respectively. There was statistically significant 
      difference between two groups (P < 0.05) while no difference existed between 
      hemocoagulase and control groups. The results of electron microscopy showed that 
      the atrial fibroblasts of EC group increased markedly versus BX group. As 
      demonstrated by RT-PCR and IHC, the expressions of MCP-1, TGF-beta1 and VEGF in BX 
      group were lower than those in EC group (P < 0.05) while no difference versus the 
      control group. CONCLUSION: As a safe and effective topical hemostatic method, BX 
      can effectively decrease inflammation response and reduce vascular remodeling and 
      narrowing in rabbits with a removal of carotid arterial adventitia. And its 
      effect mimic closely natural conditions.
FAU - Hu, Yuan-cheng
AU  - Hu YC
AD  - Department of General Surgery, Second Affiliated Hospital, Anhui Medical 
      University, Hefei 230601, China.
FAU - Wan, Sheng-yun
AU  - Wan SY
AD  - Department of General Surgery, Second Affiliated Hospital, Anhui Medical 
      University, Hefei 230601, China. Email: wshy63@sina.com.
FAU - Zhan, Yan-qing
AU  - Zhan YQ
AD  - Department of General Surgery, Second Affiliated Hospital, Anhui Medical 
      University, Hefei 230601, China.
FAU - Qin, Dan-dan
AU  - Qin DD
AD  - Department of General Surgery, Second Affiliated Hospital, Anhui Medical 
      University, Hefei 230601, China.
FAU - Ding, Yang
AU  - Ding Y
AD  - Department of General Surgery, Second Affiliated Hospital, Anhui Medical 
      University, Hefei 230601, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Hemostatics)
RN  - EC 3.4.21.- (Batroxobin)
SB  - IM
MH  - Adventitia/*surgery
MH  - Animals
MH  - Batroxobin/*therapeutic use
MH  - Carotid Artery Injuries/surgery/*therapy
MH  - *Electrocoagulation
MH  - Female
MH  - Hemostatics/therapeutic use
MH  - Male
MH  - Rabbits
EDAT- 2013/12/24 06:00
MHDA- 2014/07/22 06:00
CRDT- 2013/12/24 06:00
PHST- 2013/12/24 06:00 [entrez]
PHST- 2013/12/24 06:00 [pubmed]
PHST- 2014/07/22 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2013 Sep 17;93(35):2834-8.