PMID- 24360989
OWN - NLM
STAT- MEDLINE
DCOM- 20140331
LR  - 20140203
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 321
IP  - 2
DP  - 2014 Feb 15
TI  - Tumor necrosis factor stimulates osteoclastogenesis from human bone marrow cells 
      under hypoxic conditions.
PG  - 167-77
LID - S0014-4827(13)00513-2 [pii]
LID - 10.1016/j.yexcr.2013.11.020 [doi]
AB  - Bone homeostasis is maintained by the balance between osteoblastic bone formation 
      and osteoclastic bone resorption. In this study, we used human bone marrow cells 
      (BMCs) to investigate the role of hypoxic exposure on human osteoclast (OC) 
      formation in the presence of tumor necrosis factor (TNF). Exposing the BMCs to 
      3%, 5%, or 10% O2 in the presence of receptor activator of NF-kappaB ligand (RANKL) 
      and macrophage colony-stimulating factor (M-CSF) generated tartrate-resistant 
      acid phosphatase (TRAP)-positive multinuclear cells, consistent with OCs. The 
      addition of TNF under hypoxic conditions generated significantly greater numbers 
      of mature OCs with more nuclei than OCs generated under normoxic conditions. 
      Longer initial hypoxic exposure increased the number of OC precursor cells and 
      facilitated the differentiation of OC precursor cells into multinucleated OCs. 
      Quantitative RT-PCR analysis revealed that RANKL and TNFR1 were expressed at 
      higher levels in non-OC cells from BMCs under hypoxic conditions than under 
      normoxic conditions. Furthermore, to confirm the involvement of TNF-induced 
      signaling, we examined the effects of blocking antibodies against TNFR1 and TNFR2 
      on OC formation under hypoxic conditions. The TNFR1 antibody was observed to 
      significantly suppress OC formation. These results suggest that hypoxic exposure 
      plays an important role in TNF-induced osteoclastogenesis from human BMCs.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Nomura, Takayuki
AU  - Nomura T
AD  - Department of Orthodontics, School of Dentistry, Aichi-Gakuin University, 
      Chikusa-ku, Nagoya 464-8651, Japan; Department of Molecular Neurobiology, Nagoya 
      City University Graduate School of Medical Sciences, Mizuho-ku, Nagoya 467-8601, 
      Japan.
FAU - Aoyama, Mineyoshi
AU  - Aoyama M
AD  - Department of Molecular Neurobiology, Nagoya City University Graduate School of 
      Medical Sciences, Mizuho-ku, Nagoya 467-8601, Japan. Electronic address: 
      ao.mine@med.nagoya-cu.ac.jp.
FAU - Waguri-Nagaya, Yuko
AU  - Waguri-Nagaya Y
AD  - Department of Orthopaedic Surgery, Nagoya City University Graduate School of 
      Medical Sciences, Mizuho-ku, Nagoya 467-8601, Japan.
FAU - Goto, Yoh
AU  - Goto Y
AD  - Department of Orthodontics, School of Dentistry, Aichi-Gakuin University, 
      Chikusa-ku, Nagoya 464-8651, Japan; Department of Molecular Neurobiology, Nagoya 
      City University Graduate School of Medical Sciences, Mizuho-ku, Nagoya 467-8601, 
      Japan.
FAU - Suzuki, Mieko
AU  - Suzuki M
AD  - Department of Molecular Neurobiology, Nagoya City University Graduate School of 
      Medical Sciences, Mizuho-ku, Nagoya 467-8601, Japan.
FAU - Miyazawa, Ken
AU  - Miyazawa K
AD  - Department of Orthodontics, School of Dentistry, Aichi-Gakuin University, 
      Chikusa-ku, Nagoya 464-8651, Japan.
FAU - Asai, Kiyofumi
AU  - Asai K
AD  - Department of Molecular Neurobiology, Nagoya City University Graduate School of 
      Medical Sciences, Mizuho-ku, Nagoya 467-8601, Japan.
FAU - Goto, Shigemi
AU  - Goto S
AD  - Department of Orthodontics, School of Dentistry, Aichi-Gakuin University, 
      Chikusa-ku, Nagoya 464-8651, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131217
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Bone Marrow Cells/*drug effects/physiology
MH  - Cell Differentiation/*drug effects
MH  - Cell Hypoxia/physiology
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Gene Expression Regulation/drug effects
MH  - Humans
MH  - Osteoclasts/*drug effects/physiology
MH  - Oxygen/pharmacology
MH  - Oxygen Consumption/physiology
MH  - Time Factors
MH  - Tumor Necrosis Factor-alpha/*pharmacology
OTO - NOTNLM
OT  - Human bone marrow cells (BMCs)
OT  - Human osteoclast
OT  - Hypoxia
OT  - Tumor necrosis factor (TNF)
EDAT- 2013/12/24 06:00
MHDA- 2014/04/01 06:00
CRDT- 2013/12/24 06:00
PHST- 2013/07/18 00:00 [received]
PHST- 2013/11/06 00:00 [revised]
PHST- 2013/11/27 00:00 [accepted]
PHST- 2013/12/24 06:00 [entrez]
PHST- 2013/12/24 06:00 [pubmed]
PHST- 2014/04/01 06:00 [medline]
AID - S0014-4827(13)00513-2 [pii]
AID - 10.1016/j.yexcr.2013.11.020 [doi]
PST - ppublish
SO  - Exp Cell Res. 2014 Feb 15;321(2):167-77. doi: 10.1016/j.yexcr.2013.11.020. Epub 
      2013 Dec 17.