PMID- 24361873
OWN - NLM
STAT- MEDLINE
DCOM- 20140829
LR  - 20191210
IS  - 1879-0984 (Electronic)
IS  - 0166-0934 (Linking)
VI  - 197
DP  - 2014 Mar
TI  - Comparison of commercial enzyme-linked immunosorbent assays and fluorescent 
      microbead immunoassays for detection of antibodies against porcine reproductive 
      and respiratory syndrome virus in boars.
PG  - 63-6
LID - S0166-0934(13)00490-4 [pii]
LID - 10.1016/j.jviromet.2013.12.001 [doi]
AB  - The objective of this study was to compare the ability of two commercial 
      enzyme-linked immunosorbent assays (ELISAs) and an in-house fluorescent microbead 
      immunoassay (FMIA) to detect IgG antibodies against porcine reproductive and 
      respiratory syndrome virus (PRRSV) types 1 and 2 in serum and oral fluids from 
      boars infected experimentally. Samples from uninfected control pigs and 
      PRRSV-negative field samples were also used. Serum samples were tested by ELISAs 
      (IDEXX Se, HIPRA Se) and an in-house FMIA-Se for detection of PRRSV types 1 and 
      2. Oral fluids were tested by ELISAs (IDEXX-SO, IDEXX-OF, HIPRA-OF) for detection 
      of PRRSV types 1 and 2. Among the sera, IDEXX-Se and HIPRA-Se had similar 
      sensitivity and specificity (p>0.05); however, IDEXX-Se detected positive animals 
      earlier than HIPRA-Se (p<0.05). FMIA-Se had the highest false-positive rates in 
      known negative field samples (1/205 for IDEXX-Se, 5/205 for HIPRA-Se, and 37/205 
      for FMIA-Se; p<0.01). Serum and oral fluid samples had similar detection rates 
      and antibody kinetics using the IDEXX tests. There was a higher detection rate in 
      serum than oral fluid using the HIPRA assays. In this study, the nucleocapsid 
      protein utilized as antigen in the FMIAs yielded a low specificity. IDEXX-Se had 
      the earliest detection and similar sensitivity and specificity to the HIPRA-Se.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Gerber, Priscilla F
AU  - Gerber PF
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University, Ames, IA, USA; Laboratorio de Pesquisa em Virologia Animal, 
      Departamento de Medicina Veterinaria Preventiva, Universidade Federal de Minas 
      Gerais, Minas Gerais, Brazil.
FAU - Gimenez-Lirola, Luis G
AU  - Gimenez-Lirola LG
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Halbur, Patrick G
AU  - Halbur PG
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University, Ames, IA, USA.
FAU - Zhou, Lei
AU  - Zhou L
AD  - Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine 
      and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, USA.
FAU - Meng, Xiang-Jin
AU  - Meng XJ
AD  - Department of Biomedical Sciences and Pathobiology, Center for Molecular Medicine 
      and Infectious Diseases, College of Veterinary Medicine, Virginia Polytechnic 
      Institute and State University, Blacksburg, VA, USA.
FAU - Opriessnig, Tanja
AU  - Opriessnig T
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, Iowa State 
      University, Ames, IA, USA; The Roslin Institute and The Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Midlothian EH25 9RG, United Kingdom. 
      Electronic address: tanjaopr@iastate.edu.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131219
PL  - Netherlands
TA  - J Virol Methods
JT  - Journal of virological methods
JID - 8005839
RN  - 0 (Antibodies, Viral)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*analysis/*blood
MH  - Clinical Laboratory Techniques/*methods
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunoassay/methods
MH  - Porcine Reproductive and Respiratory Syndrome/*diagnosis
MH  - Porcine respiratory and reproductive syndrome virus/*immunology
MH  - Saliva/immunology
MH  - Sensitivity and Specificity
MH  - Serum/immunology
MH  - Sus scrofa
MH  - Swine
MH  - Veterinary Medicine/*methods
OTO - NOTNLM
OT  - Diagnosis
OT  - Oral fluid
OT  - PRRSV
OT  - Pigs
OT  - Serology
EDAT- 2013/12/24 06:00
MHDA- 2014/08/30 06:00
CRDT- 2013/12/24 06:00
PHST- 2013/07/21 00:00 [received]
PHST- 2013/10/30 00:00 [revised]
PHST- 2013/12/09 00:00 [accepted]
PHST- 2013/12/24 06:00 [entrez]
PHST- 2013/12/24 06:00 [pubmed]
PHST- 2014/08/30 06:00 [medline]
AID - S0166-0934(13)00490-4 [pii]
AID - 10.1016/j.jviromet.2013.12.001 [doi]
PST - ppublish
SO  - J Virol Methods. 2014 Mar;197:63-6. doi: 10.1016/j.jviromet.2013.12.001. Epub 
      2013 Dec 19.