PMID- 24361987
OWN - NLM
STAT- MEDLINE
DCOM- 20140902
LR  - 20140120
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1545
DP  - 2014 Jan 30
TI  - Supplement zinc as an effective treatment for spinal cord ischemia/reperfusion 
      injury in rats.
PG  - 45-53
LID - S0006-8993(13)01562-X [pii]
LID - 10.1016/j.brainres.2013.12.015 [doi]
AB  - OBJECTIVE: Brain-derived neurotrophic factor (BDNF) plays a key role in the 
      pathophysiology process and therapy of spinal cord injury (SCI). Accordingly, 
      zinc regulates the expression of BDNF and its receptor in the central nervous 
      system, the mechanism of which is still unknown. The present study investigates 
      whether supplement zinc could reduce neurological damage in a rat model, with 
      spinal cord ischemia-reperfusion (I/R) injury and how the effect of zinc 
      transporter 1(ZnT-1) was involved. METHODS: 100 Sprague-Dawley male rats were 
      randomly and evenly divided into four groups. They were subjected to spinal cord 
      ischemia by clamping the abdominal aorta for 45 min. Rats in the zinc-deficient 
      dietary model group (ZD), zinc-adequate dietary model group (ZA), and zinc-high 
      dietary model group (ZH) were given free access to purified diet, containing 5, 
      30, or 180 mg Zn/kg. Sham operation rats were subjected to laparotomy without 
      clamping of the aorta and were fed by ZA diet (30 mg Zn/kg). Neurological 
      function was scored by Tarlov's score. The spinal cord segments (L5) were 
      harvested for histological examination, auto-metallographic (AMG) analysis, 
      myeloperoxidase (MPO) activity analysis, expression of ZnT-1 and BDNF. RESULTS: 
      The rats in the ZH group have shown the higher neurological scores, slighter 
      histological changes and the attenuated MPO activity, compared with those in the 
      ZD and ZA groups at the four observation time points (p<0.05). The AMG staining 
      density in the ZH group was significantly higher than that of ZD group in 14 days 
      later after the operation. Compared with other groups, ZH group's expression of 
      Zn-T1 and BDNF were significantly increased, and was positively correlated with 
      the same time points after surgery (Spearman rho=0.403, p=0.0152.) CONCLUSION: 
      These findings suggest that zinc supplement can significantly reduce the spinal 
      cord I/R injury in rats. The mechanism may be related with restraining the MPO 
      activity and increasing of ZnT-1, which promoted the synthesis and release of 
      BDNF.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Wang, Yansong
AU  - Wang Y
AD  - Department of Orthopedics, First Affiliated Hospital of Liaoning Medical 
      University, Jinzhou City, PR China.
FAU - Su, Ribao
AU  - Su R
AD  - Department of Orthopedics, Zhoupu Hospital of Pudong New Area, Shanghai City, PR 
      China.
FAU - Lv, Gang
AU  - Lv G
AD  - Department of Orthopedics, First Affiliated Hospital of Liaoning Medical 
      University, Jinzhou City, PR China.
FAU - Cao, Yang
AU  - Cao Y
AD  - Department of Orthopedics, First Affiliated Hospital of Liaoning Medical 
      University, Jinzhou City, PR China.
FAU - Fan, Zhongkai
AU  - Fan Z
AD  - Department of Orthopedics, First Affiliated Hospital of Liaoning Medical 
      University, Jinzhou City, PR China.
FAU - Wang, Yanfeng
AU  - Wang Y
AD  - Department of Orthopedics, First Affiliated Hospital of China Medical University, 
      Shenyang City, PR China.
FAU - Zhang, Li
AU  - Zhang L
AD  - Department of Histology and Embryology, Liaoning Medical University, Jinzhou 
      City, PR China.
FAU - Yu, Deshui
AU  - Yu D
AD  - Department of Orthopedics, First Affiliated Hospital of Liaoning Medical 
      University, Jinzhou City, PR China.
FAU - Mei, Xifan
AU  - Mei X
AD  - Department of Orthopedics, First Affiliated Hospital of Liaoning Medical 
      University, Jinzhou City, PR China. Electronic address: wys990709@126.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131219
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cation Transport Proteins)
RN  - 162064-55-3 (Slc30a1 protein, rat)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Cation Transport Proteins/*metabolism
MH  - *Dietary Supplements
MH  - Male
MH  - Movement/drug effects
MH  - Peroxidase/metabolism
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reperfusion Injury/*drug therapy/pathology
MH  - Spinal Cord Ischemia/*drug therapy/pathology
MH  - Treatment Outcome
MH  - Zinc/*therapeutic use
OTO - NOTNLM
OT  - Brain-derived neurotrophic factor
OT  - Ischemia/reperfusion injury
OT  - Spinal cord
OT  - Zinc
OT  - Zinc transporter 1
EDAT- 2013/12/24 06:00
MHDA- 2014/09/03 06:00
CRDT- 2013/12/24 06:00
PHST- 2013/06/26 00:00 [received]
PHST- 2013/10/20 00:00 [revised]
PHST- 2013/12/13 00:00 [accepted]
PHST- 2013/12/24 06:00 [entrez]
PHST- 2013/12/24 06:00 [pubmed]
PHST- 2014/09/03 06:00 [medline]
AID - S0006-8993(13)01562-X [pii]
AID - 10.1016/j.brainres.2013.12.015 [doi]
PST - ppublish
SO  - Brain Res. 2014 Jan 30;1545:45-53. doi: 10.1016/j.brainres.2013.12.015. Epub 2013 
      Dec 19.