PMID- 24363345
OWN - NLM
STAT- MEDLINE
DCOM- 20140407
LR  - 20211021
IS  - 1098-5530 (Electronic)
IS  - 0021-9193 (Print)
IS  - 0021-9193 (Linking)
VI  - 196
IP  - 5
DP  - 2014 Mar
TI  - Intracellular locations of replication proteins and the origin of replication 
      during chromosome duplication in the slowly growing human pathogen Helicobacter 
      pylori.
PG  - 999-1011
LID - 10.1128/JB.01198-13 [doi]
AB  - We followed the position of the replication complex in the pathogenic bacterium 
      Helicobacter pylori using antibodies raised against the single-stranded DNA 
      binding protein (HpSSB) and the replicative helicase (HpDnaB). The position of 
      the replication origin, oriC, was also localized in growing cells by fluorescence 
      in situ hybridization (FISH) with fluorescence-labeled DNA sequences adjacent to 
      the origin. The replisome assembled at oriC near one of the cell poles, and the 
      two forks moved together toward the cell center as replication progressed in the 
      growing cell. Termination and resolution of the forks occurred near midcell, on 
      one side of the septal membrane. The duplicated copies of oriC did not separate 
      until late in elongation, when the daughter chromosomes segregated into bilobed 
      nucleoids, suggesting sister chromatid cohesion at or near the oriC region. 
      Components of the replication machinery, viz., HpDnaB and HpDnaG (DNA primase), 
      were found associated with the cell membrane. A model for the assembly and 
      location of the H. pylori replication machinery during chromosomal duplication is 
      presented.
FAU - Sharma, Atul
AU  - Sharma A
AD  - Special Centre for Molecular Medicine, Jawaharlal Nehru University, New Delhi, 
      India.
FAU - Kamran, Mohammad
AU  - Kamran M
FAU - Verma, Vijay
AU  - Verma V
FAU - Dasgupta, Santanu
AU  - Dasgupta S
FAU - Dhar, Suman Kumar
AU  - Dhar SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131220
PL  - United States
TA  - J Bacteriol
JT  - Journal of bacteriology
JID - 2985120R
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA-Binding Proteins)
SB  - IM
MH  - Bacterial Proteins/genetics/*metabolism
MH  - Cell Division/physiology
MH  - DNA Replication/*physiology
MH  - DNA, Bacterial/*physiology
MH  - DNA-Binding Proteins/genetics/*metabolism
MH  - Gene Expression Regulation, Bacterial/physiology
MH  - Helicobacter pylori/cytology/genetics/growth & development/*metabolism
MH  - Protein Transport/*physiology
PMC - PMC3957694
EDAT- 2013/12/24 06:00
MHDA- 2014/04/08 06:00
PMCR- 2014/09/01
CRDT- 2013/12/24 06:00
PHST- 2013/12/24 06:00 [entrez]
PHST- 2013/12/24 06:00 [pubmed]
PHST- 2014/04/08 06:00 [medline]
PHST- 2014/09/01 00:00 [pmc-release]
AID - JB.01198-13 [pii]
AID - 01198-13 [pii]
AID - 10.1128/JB.01198-13 [doi]
PST - ppublish
SO  - J Bacteriol. 2014 Mar;196(5):999-1011. doi: 10.1128/JB.01198-13. Epub 2013 Dec 
      20.