PMID- 24366217
OWN - NLM
STAT- MEDLINE
DCOM- 20140924
LR  - 20171116
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 9
IP  - 3
DP  - 2014 Mar
TI  - Increased expression of herpesvirus entry mediator in 1,25-dihydroxyvitamin 
      D3-treated mouse bone marrow-derived dendritic cells promotes the generation of 
      CD4(+)CD25(+)Foxp3(+) regulatory T cells.
PG  - 813-8
LID - 10.3892/mmr.2013.1874 [doi]
AB  - Dendritic cells (DCs) can initiate immune responses or induce immune tolerance. 
      1,25-dihydroxyvitamin D3 [1,25(OH)2D3] is a secosteroid hormone that can induce 
      tolerogenic dendritic cells favoring the induction of regulatory T cells (Treg). 
      The present study revealed a tolerogenic effect of 1,25(OH)2D3 on the phenotype 
      and function of ovalbumin (OVA)‑activated mouse bone marrow‑derived DCs. Three 
      inhibitory molecules associated with tolerogenic DCs, programmed death‑ligand-1 
      (PD‑L1), PD‑L2 and herpesvirus entry mediator (HVEM) and the expression of 
      co‑stimulatory molecules (CD80, CD86 and CD40) on 1,25(OH)2D3‑treated DCs were 
      examined. The levels of interleukin (IL)‑2, IL‑6 and IL‑10 secreted by 
      1,25(OH)2D3‑treated DCs were analyzed. The capability of 1,25(OH)2D3‑treated DCs 
      to induce CD4+CD25+Foxp3+ Treg and the stimulation of allogeneic CD4+ T‑cell 
      proliferation in mixed lymphocyte reaction (MLR) was studied. 1,25(OH)2D3‑treated 
      DCs induced up to 21.0‑fold upregulation of the HVEM expression and 4.1‑fold 
      enhancement of the HVEM expression upon activation with OVA [OVA‑D3/immature 
      (im)DCs]. PD‑L1 was not affected by 1,25(OH)2D3‑treated DCs and downregulated 
      PD‑L2 expression on 1,25(OH)2D3‑treated DCs and OVA‑D3/imDC. The expression of 
      co‑stimulatory molecules (CD80, CD86 and CD40) was downregulated in 
      1,25(OH)2D3‑treated DCs and OVA‑D3/imDC. Furthermore, 1,25(OH)2D3‑treated DCs 
      secreted much higher levels of IL‑10, however lower levels of IL‑2 and IL‑6 
      compared with the activated control DCs. Together with this pattern of cytokines, 
      1,25(OH)2D3‑treated DCs exhibited low allogeneic CD4+ T‑cell stimulatory activity 
      and a higher number of CD4+CD25+Foxp3+ cells in the MLR cultures but not in the 
      activated control DCs. These findings indicate that 1,25(OH)2D3 possesses the 
      immunosuppressive properties by upregulating the expression of the inhibitory 
      molecules, HVEM, which may be therapeutically useful in controlling chronic 
      immune and/or inflammatory diseases.
FAU - Huang, Yi
AU  - Huang Y
AD  - Institute of Respiratory Diseases, Xinqiao Hospital, The Third Military Medical 
      University, Chongqing 430037, P.R. China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - Department of Respiratory Medicine, Second Affiliated Hospital, Chongqing Medical 
      University, Chongqing 404000, P.R. China.
FAU - Ran, Xuemei
AU  - Ran X
AD  - Department of Respiratory Medicine, Second Affiliated Hospital, Chongqing Medical 
      University, Chongqing 404000, P.R. China.
FAU - Wang, Changzheng
AU  - Wang C
AD  - Institute of Respiratory Diseases, Xinqiao Hospital, The Third Military Medical 
      University, Chongqing 430037, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131218
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (CD4 Antigens)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - 0 (Receptors, Tumor Necrosis Factor, Member 14)
RN  - 0 (Vitamins)
RN  - 9006-59-1 (Ovalbumin)
RN  - FXC9231JVH (Calcitriol)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/*cytology
MH  - CD4 Antigens/metabolism
MH  - CD4-Positive T-Lymphocytes/immunology/metabolism
MH  - Calcitriol/*pharmacology
MH  - Dendritic Cells/cytology/*drug effects/metabolism
MH  - Down-Regulation/drug effects
MH  - Female
MH  - Forkhead Transcription Factors/metabolism
MH  - Herpesviridae/*metabolism
MH  - Interleukin-2 Receptor alpha Subunit/metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Ovalbumin/pharmacology
MH  - Receptors, Tumor Necrosis Factor, Member 14/*metabolism
MH  - T-Lymphocytes, Regulatory/*cytology/immunology/metabolism
MH  - Up-Regulation/drug effects
MH  - Vitamins/*pharmacology
EDAT- 2013/12/25 06:00
MHDA- 2014/09/25 06:00
CRDT- 2013/12/25 06:00
PHST- 2013/06/17 00:00 [received]
PHST- 2013/12/11 00:00 [accepted]
PHST- 2013/12/25 06:00 [entrez]
PHST- 2013/12/25 06:00 [pubmed]
PHST- 2014/09/25 06:00 [medline]
AID - 10.3892/mmr.2013.1874 [doi]
PST - ppublish
SO  - Mol Med Rep. 2014 Mar;9(3):813-8. doi: 10.3892/mmr.2013.1874. Epub 2013 Dec 18.