PMID- 24368227
OWN - NLM
STAT- MEDLINE
DCOM- 20140930
LR  - 20140210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 55
DP  - 2014 May 15
TI  - A droplet-based microfluidic electrochemical sensor using platinum-black 
      microelectrode and its application in high sensitive glucose sensing.
PG  - 106-12
LID - S0956-5663(13)00870-1 [pii]
LID - 10.1016/j.bios.2013.12.002 [doi]
AB  - We describe a droplet-based microfluidic electrochemical sensor using 
      platinum-black (Pt-black) microelectrode. Pt-black microelectrode was generated 
      by electrodeposition of Pt nanoparticles on bare Pt microelectrode. Scanning 
      electron microscope (SEM) image displays a flower-like microstructure of Pt 
      nanoparticels. Electrochemical impedance spectroscopy (EIS) and cyclic 
      voltammetry (CV) indicate that the Pt-black efficiently decreased the charge 
      transfer resistance and improved the electrocatalytic activity towards oxidation 
      of hydrogen peroxide (H2O2). Compared with bare Pt microelectrode, the current 
      response on Pt-black microelectrode increased 10.2 folds. The effect of applied 
      potential and electrodeposition time has been investigated in detail. The 
      proposed sensor was validated by performing enzyme activity assay in flowing 
      droplets. For demonstration, glucose oxidase (GOx) is chosen as the model enzyme, 
      which catalyzes the oxidation of beta-D-glucose to the product H2O2. The enzyme 
      activity of GOx was evaluated by measuring the electrochemical current responding 
      to various glucose concentrations. And the results indicate that this 
      microfluidic sensor holds great potential in fabricating novel glucose sensors 
      with linear response up to 43.5mM. The analytical applications of the 
      droplet-based microfluidic sensor were tested by using human blood serum samples. 
      Reproducibility, interferences, and long-term stability of the modi fi ed electrode 
      were also investigated. The present approach shows the feasibility and great 
      potentials in constructing highly sensitive and low-consumption sensors in the 
      field of droplet microfluidics.
CI  - (c) 2013 Published by Elsevier B.V.
FAU - Gu, Shuqing
AU  - Gu S
AD  - Department of chemistry, College of Sciences, Shanghai University, Shanghai 
      200444, China; Technical Center for Animal Plant and Food Inspection and 
      Quarantine, Shanghai Entry-Exit Inspection and Quarantine Bureau , Shanghai 
      200135, China; School of Materials Science and Engineering, Shanghai University, 
      Shanghai, China.
FAU - Lu, Youlan
AU  - Lu Y
AD  - Department of chemistry, College of Sciences, Shanghai University, Shanghai 
      200444, China.
FAU - Ding, Yaping
AU  - Ding Y
AD  - Department of chemistry, College of Sciences, Shanghai University, Shanghai 
      200444, China; School of Materials Science and Engineering, Shanghai University, 
      Shanghai, China. Electronic address: wdingyp@sina.com.
FAU - Li, Li
AU  - Li L
AD  - Department of chemistry, College of Sciences, Shanghai University, Shanghai 
      200444, China.
FAU - Song, Hongsheng
AU  - Song H
AD  - School of Life Sciences, Shanghai University, Shanghai, China.
FAU - Wang, Jinhua
AU  - Wang J
AD  - School of Life Sciences, Shanghai University, Shanghai, China.
FAU - Wu, Qingsheng
AU  - Wu Q
AD  - Department of Chemistry, Tongji University, Shanghai 200092, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131210
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Blood Glucose)
RN  - 0 (Enzymes, Immobilized)
RN  - 49DFR088MY (Platinum)
RN  - EC 1.1.3.4 (Glucose Oxidase)
SB  - IM
MH  - Biosensing Techniques/*instrumentation
MH  - Blood Glucose/*analysis/chemistry
MH  - Conductometry/*instrumentation
MH  - Enzymes, Immobilized/chemistry
MH  - Equipment Design
MH  - Equipment Failure Analysis
MH  - Glucose Oxidase/*chemistry
MH  - *Microelectrodes
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Platinum/*chemistry
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
OTO - NOTNLM
OT  - Droplet
OT  - Glucose oxidase
OT  - Microfluidic
OT  - Pt-black microelectrode
OT  - Sensor
EDAT- 2013/12/26 06:00
MHDA- 2014/10/01 06:00
CRDT- 2013/12/26 06:00
PHST- 2013/09/10 00:00 [received]
PHST- 2013/11/20 00:00 [revised]
PHST- 2013/12/01 00:00 [accepted]
PHST- 2013/12/26 06:00 [entrez]
PHST- 2013/12/26 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
AID - S0956-5663(13)00870-1 [pii]
AID - 10.1016/j.bios.2013.12.002 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2014 May 15;55:106-12. doi: 10.1016/j.bios.2013.12.002. Epub 
      2013 Dec 10.