PMID- 24370286
OWN - NLM
STAT- MEDLINE
DCOM- 20140414
LR  - 20131227
IS  - 1001-9391 (Print)
IS  - 1001-9391 (Linking)
VI  - 31
IP  - 11
DP  - 2013 Nov
TI  - [Changes of cell factor in bronchoalveolar lavage fluid in rats exposed to 
      silica].
PG  - 801-5
AB  - OBJECTIVE: To investigate the changes in the levels of inflammatory cytokines in 
      bronchoalveolar lavage fluid (BALF) in rats exposed to silica dust. METHODS: 
      Experimental rats were randomly divided into control group and three experimental 
      groups (doses of dust: 15, 30, and 60 mg/ml), with 42 rats in each group. Each 
      rat in the control group was treated with 1 ml of normal saline by intratracheal 
      instillation, while each rat in the experimental groups was exposed to 1 ml of 
      silica suspension by a single intratracheal instillation. Seven rats in each 
      group were killed at 1, 3, 7, 14, 21, and 28 days after exposure, and then BALF 
      was collected. Enzyme-linked immunosorbent assay was used to measure the levels 
      of interleukin (IL)-1, IL-6, IL-16, macrophage inflammatory protein-1 alpha 
      (MIP-1alpha), monocyte chemoattractant protein-1 (MCP-1), tumor necrosis factor-alpha 
      (TNF-alpha), and transforming growth factor-beta (TGF-beta). RESULTS: The levels of 
      cytokines in each experimental group were higher than those in the control group 
      at any time point. In the early stage of exposure (day 1-3), BALF IL-1 level 
      increased significantly with the increase in dust dose, and on day 14, BALF IL-6 
      and IL-16 levels increased significantly with the increase in dust dose; the 
      levels of IL-1, IL-6, and IL-16 in the experimental groups reached the peak on 
      day 14. There were significant differences in the levels of MIP-1alpha and MCP-1 
      between the experimental groups (FMIP-1alpha = 30.106, P<0.01; FMCP-1 = 17.193, 
      P<0.01). In each group, the level of MCP-1 varied significantly at different time 
      points (F = 0.618, P>0.05). On day 1-14, BALF TNF-alpha level increased with the 
      increase in dust dose, with a significant dose-response relationship (P < 0.05). 
      In each experimental group, TNF-alpha level reached the peak on day 14. On days 14, 
      21, and 28, the high-dose group had significantly higher BALF TGF-beta levels than 
      the low-dose group (P<0.05); on days 14 and 28, the high-dose group had 
      significantly higher BALF TGF-beta levels than the middle-dose group (P<0.05). 
      CONCLUSION: IL-1, IL-6, IL-16, MIP-1alpha, MCP-1, and TNF-alpha play a role in the 
      development and progression of silicosis inflammation. TGF-beta may be related to 
      (related to; associated with; correlated with) fibrosis.
FAU - Zhang, Wei
AU  - Zhang W
AD  - Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, 
      China.
FAU - Wang, Rui
AU  - Wang R
AD  - E-mail: ykywangrui@yahoo.com.cn.
FAU - Wang, Xin
AU  - Wang X
FAU - Zhou, Xiao-bao
AU  - Zhou XB
FAU - Wang, Hui
AU  - Wang H
FAU - Zhang, Hai-dong
AU  - Zhang HD
FAU - Liu, Zheng-liang
AU  - Liu ZL
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
JT  - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng 
      zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
JID - 8410840
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL3)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-16)
RN  - 0 (Interleukin-6)
RN  - 0 (Tgfb1 protein, rat)
RN  - 0 (Transforming Growth Factor beta1)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Bronchoalveolar Lavage Fluid/*chemistry
MH  - Chemokine CCL2/metabolism
MH  - Chemokine CCL3/metabolism
MH  - Cytokines/*metabolism
MH  - Interleukin-1/metabolism
MH  - Interleukin-16/metabolism
MH  - Interleukin-6/metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Silicon Dioxide/*toxicity
MH  - Silicosis/*metabolism
MH  - Transforming Growth Factor beta1/metabolism
EDAT- 2013/12/29 06:00
MHDA- 2014/04/15 06:00
CRDT- 2013/12/28 06:00
PHST- 2013/12/28 06:00 [entrez]
PHST- 2013/12/29 06:00 [pubmed]
PHST- 2014/04/15 06:00 [medline]
PST - ppublish
SO  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013 Nov;31(11):801-5.