PMID- 2437083
OWN - NLM
STAT- MEDLINE
DCOM- 19870610
LR  - 20190828
IS  - 0301-5564 (Print)
IS  - 0301-5564 (Linking)
VI  - 86
IP  - 4
DP  - 1987
TI  - Silver enhancement of protein A-gold probes on resin-embedded ultrathin sections. 
      An electron microscopic localization of mouse mammary tumor virus (MMTV) 
      antigens.
PG  - 371-3
AB  - A simple method is described allowing the enhancement of the visibility of small 
      gold probes for the electron microscopy. This method, which allows the silver 
      intensification of gold directly on epon-embedded ultrathin sections, was used 
      for the electron microscopic localization of Mouse Mammary Tumor Virus (MMTV) 
      antigens in cultured cells derived from GR and BALB/cfRIII mouse mammary tumors. 
      After the immunostaining with the preembedding protein A-gold technique, the 
      ultrathin sections, placed on 200 mesh copper grids, were rehydrated and exposed 
      to a photographic developer containing silver nitrate. During this physical 
      development gold particles are incapsulated in growing shells of metallic silver, 
      which gradually become more and more visible. We were able to obtain a heavy 
      labelling of the viral particles, well visible even at low magnification, with a 
      negligeable background staining. The present technique can be useful whenever it 
      is necessary to use the smallest gold probes today available.
FAU - Marchetti, A
AU  - Marchetti A
FAU - Bistocchi, M
AU  - Bistocchi M
FAU - Tognetti, A R
AU  - Tognetti AR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Histochemistry
JT  - Histochemistry
JID - 0411300
RN  - 0 (Antigens, Viral, Tumor)
RN  - 0 (Staphylococcal Protein A)
RN  - 3M4G523W1G (Silver)
RN  - 7440-57-5 (Gold)
SB  - IM
MH  - Animals
MH  - Antigens, Viral, Tumor/*analysis
MH  - Cells, Cultured
MH  - Gold
MH  - *Immunologic Techniques
MH  - Mammary Neoplasms, Experimental/*immunology
MH  - Mammary Tumor Virus, Mouse/*immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Microscopy, Electron/*methods
MH  - Silver
MH  - *Staining and Labeling
MH  - Staphylococcal Protein A
EDAT- 1987/01/01 00:00
MHDA- 1987/01/01 00:01
CRDT- 1987/01/01 00:00
PHST- 1987/01/01 00:00 [pubmed]
PHST- 1987/01/01 00:01 [medline]
PHST- 1987/01/01 00:00 [entrez]
AID - 10.1007/BF00494995 [doi]
PST - ppublish
SO  - Histochemistry. 1987;86(4):371-3. doi: 10.1007/BF00494995.