PMID- 24370951
OWN - NLM
STAT- MEDLINE
DCOM- 20150219
LR  - 20140317
IS  - 1095-9319 (Electronic)
IS  - 0026-2862 (Linking)
VI  - 92
DP  - 2014 Mar
TI  - Hepatocyte growth factor enhances the barrier function in primary cultures of rat 
      brain microvascular endothelial cells.
PG  - 41-9
LID - S0026-2862(13)00205-7 [pii]
LID - 10.1016/j.mvr.2013.12.004 [doi]
AB  - The effects of hepatocyte growth factor (HGF) on barrier functions were 
      investigated by a blood-brain barrier (BBB) in vitro model comprising a primary 
      culture of rat brain capillary endothelial cells (RBEC). In order to examine the 
      response of the peripheral endothelial cells to HGF, human umbilical vascular 
      endothelial cells (HUVEC) and human dermal microvascular endothelial cells 
      (HMVEC) were also treated with HGF. HGF decreased the permeability of RBEC to 
      sodium fluorescein and Evans blue albumin, and dose-dependently increased 
      transendothelial electrical resistance (TEER) in RBEC. HGF altered the 
      immunochemical staining pattern of F-actin bands and made ZO-1 staining more 
      distinct on the linear cell borders in RBEC. In contrast, HGF increased sodium 
      fluorescein and Evans blue albumin permeability in HMVEC and HUVEC, and decreased 
      TEER in HMVEC. In HMVEC, HGF reduced cortical actin bands and increased stress 
      fiber density, and increased the zipper-like appearance of ZO-1 staining. Western 
      blot analysis showed that HGF significantly increased the amount of ZO-1 and 
      VE-cadherin. HGF seems to act on the BBB to strengthen BBB integrity. These 
      findings indicated that cytoskeletal rearrangement and cell-cell adhesion, such 
      as through VE-cadherin and ZO-1, are candidate mechanisms for the influence of 
      HGF on the BBB. The possibility that HGF has therapeutic significance in 
      protecting the BBB from damage needs to be considered.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Yamada, Narumi
AU  - Yamada N
AD  - Clinical Research Center, National Hospital Organization Nagasaki Medical Center, 
      2-1001-1 Kubara, Omura 856-8562, Japan; Department of Hepatology, Nagasaki 
      University Graduate School of Biomedical Sciences, 2-1001-1 Kubara, Omura 
      856-8562, Japan.
FAU - Nakagawa, Shinsuke
AU  - Nakagawa S
AD  - Department of Pharmacology, Nagasaki University Graduate School of Biomedical 
      Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan; BBB Laboratory, 
      PharmaCo-Cell Company, Ltd., 1-43 Dejima, Nagasaki 850-0862, Japan. Electronic 
      address: shin3@nagasaki-u.ac.jp.
FAU - Horai, Shoji
AU  - Horai S
AD  - Department of Pharmacology, Nagasaki University Graduate School of Biomedical 
      Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
FAU - Tanaka, Kunihiko
AU  - Tanaka K
AD  - Department of Pharmacology, Nagasaki University Graduate School of Biomedical 
      Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan.
FAU - Deli, Maria A
AU  - Deli MA
AD  - Laboratory of Molecular Neurobiology, Institute of Biophysics, Biological 
      Research Center, Hungarian Academy of Sciences, Szeged, Hungary.
FAU - Yatsuhashi, Hiroshi
AU  - Yatsuhashi H
AD  - Clinical Research Center, National Hospital Organization Nagasaki Medical Center, 
      2-1001-1 Kubara, Omura 856-8562, Japan; Department of Hepatology, Nagasaki 
      University Graduate School of Biomedical Sciences, 2-1001-1 Kubara, Omura 
      856-8562, Japan.
FAU - Niwa, Masami
AU  - Niwa M
AD  - Department of Pharmacology, Nagasaki University Graduate School of Biomedical 
      Sciences, 1-12-4 Sakamoto, Nagasaki 852-8523, Japan; BBB Laboratory, 
      PharmaCo-Cell Company, Ltd., 1-43 Dejima, Nagasaki 850-0862, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131223
PL  - United States
TA  - Microvasc Res
JT  - Microvascular research
JID - 0165035
RN  - 0 (Actins)
RN  - 0 (Antigens, CD)
RN  - 0 (Cadherins)
RN  - 0 (Tight Junction Proteins)
RN  - 0 (Tjp1 protein, rat)
RN  - 0 (Zonula Occludens-1 Protein)
RN  - 0 (cadherin 5)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
SB  - IM
MH  - Actins/metabolism
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Blood-Brain Barrier/*cytology/drug effects/*physiology
MH  - Brain/*blood supply
MH  - Cadherins/metabolism
MH  - Capillary Permeability/drug effects/physiology
MH  - Cell Adhesion/physiology
MH  - Cells, Cultured
MH  - Endothelial Cells/drug effects/*physiology
MH  - Hepatocyte Growth Factor/pharmacology/*physiology
MH  - Human Umbilical Vein Endothelial Cells
MH  - Humans
MH  - Microvessels/cytology/drug effects/physiology
MH  - Primary Cell Culture
MH  - Proto-Oncogene Proteins c-met/metabolism
MH  - Rats
MH  - Tight Junction Proteins/metabolism
MH  - Zonula Occludens-1 Protein/metabolism
EDAT- 2013/12/29 06:00
MHDA- 2015/02/20 06:00
CRDT- 2013/12/28 06:00
PHST- 2013/09/25 00:00 [received]
PHST- 2013/12/04 00:00 [revised]
PHST- 2013/12/16 00:00 [accepted]
PHST- 2013/12/28 06:00 [entrez]
PHST- 2013/12/29 06:00 [pubmed]
PHST- 2015/02/20 06:00 [medline]
AID - S0026-2862(13)00205-7 [pii]
AID - 10.1016/j.mvr.2013.12.004 [doi]
PST - ppublish
SO  - Microvasc Res. 2014 Mar;92:41-9. doi: 10.1016/j.mvr.2013.12.004. Epub 2013 Dec 
      23.