PMID- 24372629
OWN - NLM
STAT- MEDLINE
DCOM- 20141121
LR  - 20140826
IS  - 1600-0463 (Electronic)
IS  - 0903-4641 (Linking)
VI  - 122
IP  - 9
DP  - 2014 Sep
TI  - Analysis of HER2 status in breast carcinoma by fully automated HER2 fluorescence 
      in situ hybridization (FISH): comparison of two immunohistochemical tests and 
      manual FISH.
PG  - 755-60
LID - 10.1111/apm.12215 [doi]
AB  - Easy and accurate HER2 testing is essential when considering the prognostic and 
      predictive significance of HER2 in breast cancer. The use of a fully automated, 
      quantitative FISH assay would be helpful to detect HER2 amplification in breast 
      cancer tissue specimens with reduced inter-laboratory variability. We compared 
      the concordance of HER2 status as assessed by an automated FISH staining system 
      to manual FISH testing. Using 60 formalin-fixed paraffin-embedded breast 
      carcinoma specimens, we assessed HER2 immunoexpression with two antibodies (DAKO 
      HercepTest and CB11). In addition, HER2 status was evaluated with automated FISH 
      using the Leica FISH System for BOND and a manual FISH using the Abbott 
      PathVysion DNA Probe Kit. All but one specimen were successfully stained using 
      both FISH methods. When the data were divided into two groups according to 
      HER2/CEP17 ratio, positive and negative, the results from both the automated and 
      manual FISH techniques were identical for all 59 evaluable specimens. The HER2 
      and CEP17 copy numbers and HER2/CEP17 ratio showed great agreement between both 
      FISH methods. The automated FISH technique was interpretable with signal 
      intensity similar to those of the manual FISH technique. In contrast with manual 
      FISH, the automated FISH technique showed well-preserved architecture due to low 
      membrane digestion. HER2 immunohistochemistry and FISH results showed substantial 
      significant agreement (kappa = 1.0, p < 0.001). HER2 status can be reliably 
      determined using a fully automated HER2 FISH system with high concordance to the 
      well-established manual FISH method. Because of stable signal intensity and high 
      staining quality, the automated FISH technique may be more appropriate than 
      manual FISH for routine applications.
CI  - (c) 2013 APMIS. Published by John Wiley & Sons Ltd.
FAU - Yoon, Nara
AU  - Yoon N
AD  - Department of Pathology, Samsung Medical Center, Sungkyunkwan University College 
      of Medicine, Seoul, Korea.
FAU - Do, In-Gu
AU  - Do IG
FAU - Cho, Eun Yoon
AU  - Cho EY
LA  - eng
PT  - Comparative Study
PT  - Journal Article
DEP - 20131220
PL  - Denmark
TA  - APMIS
JT  - APMIS : acta pathologica, microbiologica, et immunologica Scandinavica
JID - 8803400
RN  - 0 (Antibodies)
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Adult
MH  - Antibodies/immunology
MH  - *Automation, Laboratory
MH  - Breast Neoplasms/*diagnosis
MH  - Diagnostic Tests, Routine/methods
MH  - Female
MH  - Gene Amplification
MH  - Humans
MH  - Immunohistochemistry/methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Paraffin Embedding
MH  - Receptor, ErbB-2/*analysis/immunology/metabolism
OTO - NOTNLM
OT  - Genes
OT  - automation
OT  - breast
OT  - carcinoma
OT  - ductal
OT  - erbB-2
OT  - fluorescence
OT  - in situ hybridization
EDAT- 2014/01/01 06:00
MHDA- 2014/12/15 06:00
CRDT- 2013/12/31 06:00
PHST- 2013/03/13 00:00 [received]
PHST- 2013/10/21 00:00 [accepted]
PHST- 2013/12/31 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2014/12/15 06:00 [medline]
AID - 10.1111/apm.12215 [doi]
PST - ppublish
SO  - APMIS. 2014 Sep;122(9):755-60. doi: 10.1111/apm.12215. Epub 2013 Dec 20.