PMID- 24374218
OWN - NLM
STAT- MEDLINE
DCOM- 20140612
LR  - 20211021
IS  - 1932-7420 (Electronic)
IS  - 1550-4131 (Print)
IS  - 1550-4131 (Linking)
VI  - 19
IP  - 1
DP  - 2014 Jan 7
TI  - Local proliferation of macrophages contributes to obesity-associated adipose 
      tissue inflammation.
PG  - 162-171
LID - 10.1016/j.cmet.2013.11.017 [doi]
AB  - Adipose tissue (AT) of obese mice and humans accumulates immune cells, which 
      secrete cytokines that can promote insulin resistance. AT macrophages (ATMs) are 
      thought to originate from bone-marrow-derived monocytes, which infiltrate the 
      tissue from the circulation. Here, we show that a major fraction of macrophages 
      unexpectedly undergo cell division locally within AT, as detected by Ki67 
      expression and 5-ethynyl-2'-deoxyuridine incorporation. Macrophages within the 
      visceral AT (VAT), but not those in other tissues (including liver and spleen), 
      displayed increased proliferation in obesity. Importantly, depletion of blood 
      monocytes had no impact on ATM content, whereas their proliferation in situ 
      continued. Treatment with monocyte chemotactic protein 1 (MCP-1) induced 
      macrophage cell division in AT explants, whereas mcp-1 deficiency in vivo 
      decreased ATM proliferation. These results reveal that, in addition to blood 
      monocyte recruitment, in situ proliferation driven by MCP-1 is an important 
      process by which macrophages accumulate in the VAT in obesity.
CI  - Copyright (c) 2014 Elsevier Inc. All rights reserved.
FAU - Amano, Shinya U
AU  - Amano SU
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
FAU - Cohen, Jessica L
AU  - Cohen JL
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
FAU - Vangala, Pranitha
AU  - Vangala P
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
FAU - Tencerova, Michaela
AU  - Tencerova M
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
FAU - Nicoloro, Sarah M
AU  - Nicoloro SM
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
FAU - Yawe, Joseph C
AU  - Yawe JC
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
FAU - Shen, Yuefei
AU  - Shen Y
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
FAU - Czech, Michael P
AU  - Czech MP
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
FAU - Aouadi, Myriam
AU  - Aouadi M
AD  - Program in Molecular Medicine, University of Massachusetts Medical School, 
      Worcester, Massachusetts 01605, USA.
LA  - eng
GR  - DK085753/DK/NIDDK NIH HHS/United States
GR  - R01 DK085753/DK/NIDDK NIH HHS/United States
GR  - F32 DK098879/DK/NIDDK NIH HHS/United States
GR  - P01 AI046629/AI/NIAID NIH HHS/United States
GR  - F32DK098879/DK/NIDDK NIH HHS/United States
GR  - AI046629/AI/NIAID NIH HHS/United States
GR  - DK325220/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20131226
PL  - United States
TA  - Cell Metab
JT  - Cell metabolism
JID - 101233170
RN  - 0 (Biomarkers)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Ki-67 Antigen)
SB  - IM
MH  - Adipose Tissue/*pathology
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Cell Division
MH  - Cell Proliferation
MH  - Chemokine CCL2/metabolism
MH  - Humans
MH  - Inflammation/*pathology
MH  - Ki-67 Antigen/metabolism
MH  - Macrophages/metabolism/*pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Monocytes/metabolism
MH  - Obesity/metabolism/*pathology
PMC - PMC3931314
MID - NIHMS552740
EDAT- 2014/01/01 06:00
MHDA- 2014/06/13 06:00
PMCR- 2015/01/07
CRDT- 2013/12/31 06:00
PHST- 2012/08/22 00:00 [received]
PHST- 2013/06/04 00:00 [revised]
PHST- 2013/11/15 00:00 [accepted]
PHST- 2013/12/31 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2014/06/13 06:00 [medline]
PHST- 2015/01/07 00:00 [pmc-release]
AID - 10.1016/j.cmet.2013.11.017 [doi]
PST - ppublish
SO  - Cell Metab. 2014 Jan 7;19(1):162-171. doi: 10.1016/j.cmet.2013.11.017. Epub 2013 
      Dec 26.