PMID- 24374587
OWN - NLM
STAT- MEDLINE
DCOM- 20140910
LR  - 20171116
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 473-474
DP  - 2014 Mar 1
TI  - Impact of Ag and Al(2)O(3) nanoparticles on soil organisms: in vitro and soil 
      experiments.
PG  - 254-61
LID - S0048-9697(13)01497-6 [pii]
LID - 10.1016/j.scitotenv.2013.12.043 [doi]
AB  - In vitro analyses were conducted to assess the impact of Al2O3 and Ag 
      nanoparticles on two common soil bacteria, Bacillus cereus and Pseudomonas 
      stutzeri. Al2O3 nanoparticles did not show significant toxicity at any dose or 
      time assayed, whereas exposure to 5 mg L(-1) Ag nanoparticles for 48 h caused 
      bactericidal effects. Moreover, alterations at the morphological level were 
      observed by transmission electron microscopy (TEM); Ag but not Al2O3 
      nanoparticles evoked the entrance of B. cereus cells in an early sporulation 
      stage and both nanoparticles penetrated P. stutzeri cells. At the molecular 
      level, a dramatic increase (8.2-fold) in katB gene expression was found in P. 
      stutzeri following Al2O3 nanoparticles exposure, indicative of an oxidative 
      stress-defence system enhancement in this bacterium. In the microcosm experiment, 
      using two different natural soils, Al2O3 or Ag nanoparticles did not affect the 
      Caenorhabditis elegans toxicity endpoints growth, survival, or reproduction. 
      However, differences in microbial phylogenetic compositions were detected by 
      fluorescence in situ hybridization (FISH). The use of katB- and pykA-based 
      sequences showed that the microbial transcriptional response to nanoparticle 
      exposure decreased, suggesting a decrease in cellular activity. These changes 
      were attributable to both the nanoparticles treatment and soil characteristics, 
      highlighting the importance of considering the soil matrix on a case by case 
      basis.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Fajardo, C
AU  - Fajardo C
AD  - Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Electronic 
      address: carmen.fajardo@vet.ucm.es.
FAU - Sacca, M L
AU  - Sacca ML
AD  - Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain; Campus de 
      Excelencia Internacional de Moncloa, 28040 Madrid, Spain. Electronic address: 
      marialudovicasacca@pdi.ucm.es.
FAU - Costa, G
AU  - Costa G
AD  - Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Electronic 
      address: costag@vet.ucm.es.
FAU - Nande, M
AU  - Nande M
AD  - Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Electronic 
      address: mnande@vet.ucm.es.
FAU - Martin, M
AU  - Martin M
AD  - Facultad de Veterinaria, Universidad Complutense, 28040 Madrid, Spain. Electronic 
      address: margamar@vet.ucm.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131227
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
RN  - 0 (Soil)
RN  - 0 (Soil Pollutants)
RN  - 3M4G523W1G (Silver)
RN  - CPD4NFA903 (Aluminum)
SB  - IM
MH  - Aluminum/*toxicity
MH  - Animals
MH  - Caenorhabditis elegans/drug effects
MH  - Gene Expression/drug effects
MH  - Metal Nanoparticles/*toxicity
MH  - Oxidative Stress
MH  - Silver/*toxicity
MH  - Soil
MH  - Soil Pollutants/*toxicity
OTO - NOTNLM
OT  - Bacteria
OT  - C. elegans
OT  - Cytotoxicity
OT  - Nanoparticles
OT  - Soil
EDAT- 2014/01/01 06:00
MHDA- 2014/09/11 06:00
CRDT- 2013/12/31 06:00
PHST- 2013/10/16 00:00 [received]
PHST- 2013/12/09 00:00 [revised]
PHST- 2013/12/10 00:00 [accepted]
PHST- 2013/12/31 06:00 [entrez]
PHST- 2014/01/01 06:00 [pubmed]
PHST- 2014/09/11 06:00 [medline]
AID - S0048-9697(13)01497-6 [pii]
AID - 10.1016/j.scitotenv.2013.12.043 [doi]
PST - ppublish
SO  - Sci Total Environ. 2014 Mar 1;473-474:254-61. doi: 
      10.1016/j.scitotenv.2013.12.043. Epub 2013 Dec 27.